Characterization of the cognitive and motor changes revealed by the elevated plus maze in an experimental rat model of radiation-induced brain injury
Background: Experimental models are needed to better understand the pathophysiology of neurodegenerative diseases to develop novel therapeutics. The neuropathology and clinical signs of acute radiation syndrome resemble those of neurodegenerative conditions. We characterized elevated plus maze (EPM)...
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doaj-13820efc8462424086ac6e0394eccea22021-01-08T02:50:29ZengWolters Kluwer Medknow PublicationsAdvanced Biomedical Research2277-91752020-01-0191727210.4103/abr.abr_62_20Characterization of the cognitive and motor changes revealed by the elevated plus maze in an experimental rat model of radiation-induced brain injuryAlfred K NjamnshiNene AhidjoLeonard NgarkaLeonard N NforMichel K MengnjoWepnyu Y NjamnshiJonas Guy Basseguin AtchouGodwin Y TatahLouis M MbakuFaustin Dong À ZokPaul F Seke EtetBackground: Experimental models are needed to better understand the pathophysiology of neurodegenerative diseases to develop novel therapeutics. The neuropathology and clinical signs of acute radiation syndrome resemble those of neurodegenerative conditions. We characterized elevated plus maze (EPM) indicators of cognitive and motor impairment in rats exposed to brain-damaging doses of gamma radiation to develop a model for neurological component of the acute radiation syndrome. Materials and Methods: Technetium 99 m was administered once through tail vein to male Wistar rats to reach an absorbed dose of Gamma radiation of 667 mGy (66.7Rad). Animal performance in the EPM was assessed every 14 days. Rats were observed for 9 weeks for the occurrence of systemic and neurological signs. Comparisons were done between irradiated and nonirradiated rats, and in each group with baseline performance. Results: EPM indicators of cognitive and motor impairment, anxiety, and depression were observed concomitantly and increased with the severity of acute radiation syndrome-like systemic and neurological signs. Alterations in EPM indicators appeared 3 weeks postirradiation and their severity increased with time. Notably, arm transitions and the distance covered in the maze were decreased (−56.71% and −73.62%, P < 0.001), as well as open arm entries and time spent in open arms (−77.78% and −76.19%, P < 0.05) and the indicator of thigmotaxis rearing (−92.45, P < 0.001). Conclusions: Our results suggest that irradiated rat performance in the EPM paradigm reflects disease severity and could be used to perform both acute and subchronic pharmacological studies in acute radiation syndrome-like diseases in rats.http://www.advbiores.net/article.asp?issn=2277-9175;year=2020;volume=9;issue=1;spage=72;epage=72;aulast=Njamnshianxietyacute radiation syndromecognitive dysfunctiondepressionelevated plus mazegamma raysrats |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Alfred K Njamnshi Nene Ahidjo Leonard Ngarka Leonard N Nfor Michel K Mengnjo Wepnyu Y Njamnshi Jonas Guy Basseguin Atchou Godwin Y Tatah Louis M Mbaku Faustin Dong À Zok Paul F Seke Etet |
spellingShingle |
Alfred K Njamnshi Nene Ahidjo Leonard Ngarka Leonard N Nfor Michel K Mengnjo Wepnyu Y Njamnshi Jonas Guy Basseguin Atchou Godwin Y Tatah Louis M Mbaku Faustin Dong À Zok Paul F Seke Etet Characterization of the cognitive and motor changes revealed by the elevated plus maze in an experimental rat model of radiation-induced brain injury Advanced Biomedical Research anxiety acute radiation syndrome cognitive dysfunction depression elevated plus maze gamma rays rats |
author_facet |
Alfred K Njamnshi Nene Ahidjo Leonard Ngarka Leonard N Nfor Michel K Mengnjo Wepnyu Y Njamnshi Jonas Guy Basseguin Atchou Godwin Y Tatah Louis M Mbaku Faustin Dong À Zok Paul F Seke Etet |
author_sort |
Alfred K Njamnshi |
title |
Characterization of the cognitive and motor changes revealed by the elevated plus maze in an experimental rat model of radiation-induced brain injury |
title_short |
Characterization of the cognitive and motor changes revealed by the elevated plus maze in an experimental rat model of radiation-induced brain injury |
title_full |
Characterization of the cognitive and motor changes revealed by the elevated plus maze in an experimental rat model of radiation-induced brain injury |
title_fullStr |
Characterization of the cognitive and motor changes revealed by the elevated plus maze in an experimental rat model of radiation-induced brain injury |
title_full_unstemmed |
Characterization of the cognitive and motor changes revealed by the elevated plus maze in an experimental rat model of radiation-induced brain injury |
title_sort |
characterization of the cognitive and motor changes revealed by the elevated plus maze in an experimental rat model of radiation-induced brain injury |
publisher |
Wolters Kluwer Medknow Publications |
series |
Advanced Biomedical Research |
issn |
2277-9175 |
publishDate |
2020-01-01 |
description |
Background: Experimental models are needed to better understand the pathophysiology of neurodegenerative diseases to develop novel therapeutics. The neuropathology and clinical signs of acute radiation syndrome resemble those of neurodegenerative conditions. We characterized elevated plus maze (EPM) indicators of cognitive and motor impairment in rats exposed to brain-damaging doses of gamma radiation to develop a model for neurological component of the acute radiation syndrome. Materials and Methods: Technetium 99 m was administered once through tail vein to male Wistar rats to reach an absorbed dose of Gamma radiation of 667 mGy (66.7Rad). Animal performance in the EPM was assessed every 14 days. Rats were observed for 9 weeks for the occurrence of systemic and neurological signs. Comparisons were done between irradiated and nonirradiated rats, and in each group with baseline performance. Results: EPM indicators of cognitive and motor impairment, anxiety, and depression were observed concomitantly and increased with the severity of acute radiation syndrome-like systemic and neurological signs. Alterations in EPM indicators appeared 3 weeks postirradiation and their severity increased with time. Notably, arm transitions and the distance covered in the maze were decreased (−56.71% and −73.62%, P < 0.001), as well as open arm entries and time spent in open arms (−77.78% and −76.19%, P < 0.05) and the indicator of thigmotaxis rearing (−92.45, P < 0.001). Conclusions: Our results suggest that irradiated rat performance in the EPM paradigm reflects disease severity and could be used to perform both acute and subchronic pharmacological studies in acute radiation syndrome-like diseases in rats. |
topic |
anxiety acute radiation syndrome cognitive dysfunction depression elevated plus maze gamma rays rats |
url |
http://www.advbiores.net/article.asp?issn=2277-9175;year=2020;volume=9;issue=1;spage=72;epage=72;aulast=Njamnshi |
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