Mapping of the 18S and 5S ribosomal RNA genes in Astyanax altiparanae Garutti & Britski, 2000 (Teleostei, Characidae) from the upper Paraná river basin, Brazil
Fluorescence in situ hybridization (FISH) was undertaken in order to determinate the chromosomal distribution pattern of 18S and 5S ribosomal DNAs (rDNA) in four populations of the characid fish Astyanax altiparanae from the upper Paraná river basin, Brazil. The 18S rDNA probe FISH revealed numerica...
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Sociedade Brasileira de Genética
2006-01-01
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doaj-1393e4fd5aa0473bb79e9f3086cff6442020-11-25T02:51:18ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1415-47571678-46852006-01-0129346446810.1590/S1415-47572006000300011Mapping of the 18S and 5S ribosomal RNA genes in Astyanax altiparanae Garutti & Britski, 2000 (Teleostei, Characidae) from the upper Paraná river basin, BrazilCarlos Alexandre FernandesIsabel Cristina Martins-SantosFluorescence in situ hybridization (FISH) was undertaken in order to determinate the chromosomal distribution pattern of 18S and 5S ribosomal DNAs (rDNA) in four populations of the characid fish Astyanax altiparanae from the upper Paraná river basin, Brazil. The 18S rDNA probe FISH revealed numerical and positional variations among specimens from the Keçaba stream compared to specimens of the other populations studied. In contrast to the variable 18S rDNA distribution pattern, highly stable chromosomal positioning of the 5S rDNA sites was observed in the four A. altiparanae populations. Divergence in the distribution pattern of 18S and 5S rDNA sites is also discussed.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000300011Astyanax altiparanaefluorescence in situ hybridization (FISH)18S rDNA5S rDNAsequential Ag-NOR |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Carlos Alexandre Fernandes Isabel Cristina Martins-Santos |
spellingShingle |
Carlos Alexandre Fernandes Isabel Cristina Martins-Santos Mapping of the 18S and 5S ribosomal RNA genes in Astyanax altiparanae Garutti & Britski, 2000 (Teleostei, Characidae) from the upper Paraná river basin, Brazil Genetics and Molecular Biology Astyanax altiparanae fluorescence in situ hybridization (FISH) 18S rDNA 5S rDNA sequential Ag-NOR |
author_facet |
Carlos Alexandre Fernandes Isabel Cristina Martins-Santos |
author_sort |
Carlos Alexandre Fernandes |
title |
Mapping of the 18S and 5S ribosomal RNA genes in Astyanax altiparanae Garutti & Britski, 2000 (Teleostei, Characidae) from the upper Paraná river basin, Brazil |
title_short |
Mapping of the 18S and 5S ribosomal RNA genes in Astyanax altiparanae Garutti & Britski, 2000 (Teleostei, Characidae) from the upper Paraná river basin, Brazil |
title_full |
Mapping of the 18S and 5S ribosomal RNA genes in Astyanax altiparanae Garutti & Britski, 2000 (Teleostei, Characidae) from the upper Paraná river basin, Brazil |
title_fullStr |
Mapping of the 18S and 5S ribosomal RNA genes in Astyanax altiparanae Garutti & Britski, 2000 (Teleostei, Characidae) from the upper Paraná river basin, Brazil |
title_full_unstemmed |
Mapping of the 18S and 5S ribosomal RNA genes in Astyanax altiparanae Garutti & Britski, 2000 (Teleostei, Characidae) from the upper Paraná river basin, Brazil |
title_sort |
mapping of the 18s and 5s ribosomal rna genes in astyanax altiparanae garutti & britski, 2000 (teleostei, characidae) from the upper paraná river basin, brazil |
publisher |
Sociedade Brasileira de Genética |
series |
Genetics and Molecular Biology |
issn |
1415-4757 1678-4685 |
publishDate |
2006-01-01 |
description |
Fluorescence in situ hybridization (FISH) was undertaken in order to determinate the chromosomal distribution pattern of 18S and 5S ribosomal DNAs (rDNA) in four populations of the characid fish Astyanax altiparanae from the upper Paraná river basin, Brazil. The 18S rDNA probe FISH revealed numerical and positional variations among specimens from the Keçaba stream compared to specimens of the other populations studied. In contrast to the variable 18S rDNA distribution pattern, highly stable chromosomal positioning of the 5S rDNA sites was observed in the four A. altiparanae populations. Divergence in the distribution pattern of 18S and 5S rDNA sites is also discussed. |
topic |
Astyanax altiparanae fluorescence in situ hybridization (FISH) 18S rDNA 5S rDNA sequential Ag-NOR |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000300011 |
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AT carlosalexandrefernandes mappingofthe18sand5sribosomalrnagenesinastyanaxaltiparanaegaruttibritski2000teleosteicharacidaefromtheupperparanariverbasinbrazil AT isabelcristinamartinssantos mappingofthe18sand5sribosomalrnagenesinastyanaxaltiparanaegaruttibritski2000teleosteicharacidaefromtheupperparanariverbasinbrazil |
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