Protective role of dexmedetomidine in unmethylated CpG-induced inflammation responses in BV2 microglia cells
Unmethylated CpG DNA, as a stimulatory molecule, has potent pro-inflammatory effects in the central nervous system (CNS). Dexmedetomidine (DEX) has been confirmed to exert anti-inflammatory effects in CNS. Our study was aimed to explore the effects of DEX on tumor necrosis factor-α (TNF-α) expressio...
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doaj-13983f4a3c474fb5bfe8674fdacf0c042020-11-25T00:19:11ZengTermedia Publishing HouseFolia Neuropathologica1641-46401509-572X2016-12-0154438239110.5114/fn.2016.6481728979Protective role of dexmedetomidine in unmethylated CpG-induced inflammation responses in BV2 microglia cellsChen ChenYanning QianUnmethylated CpG DNA, as a stimulatory molecule, has potent pro-inflammatory effects in the central nervous system (CNS). Dexmedetomidine (DEX) has been confirmed to exert anti-inflammatory effects in CNS. Our study was aimed to explore the effects of DEX on tumor necrosis factor-α (TNF-α) expression in unmethylated CpG DNA-challenged microglia. In vivo, after 3 d intracisternal injection of ODN1668, we evaluated the severity of meningitis with or without DEX via pathobiology method and detected the expression of TNF-α from molecular and protein levels. In vitro, we explored whether the ODN1668 could activate microglia to express TNF-α and the inhibition mechanism of DEX. Our results demonstrated that DEX could alleviate the severity of ODN1668-induced meningitis. And while BV2 microglia was stimulated by ODN1668 for different time, TNF-α was increased in mRNA and protein levels but the effect was attenuated by DEX via decreasing phosphorylated AKT and ERKhttps://www.termedia.pl/Protective-role-of-dexmedetomidine-in-unmethylated-CpG-induced-inflammation-responses-in-BV2-microglia-cells,20,28979,1,1.htmlDEX unmethylated CpG DNA microglia |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Chen Chen Yanning Qian |
spellingShingle |
Chen Chen Yanning Qian Protective role of dexmedetomidine in unmethylated CpG-induced inflammation responses in BV2 microglia cells Folia Neuropathologica DEX unmethylated CpG DNA microglia |
author_facet |
Chen Chen Yanning Qian |
author_sort |
Chen Chen |
title |
Protective role of dexmedetomidine in unmethylated CpG-induced inflammation responses in BV2 microglia cells |
title_short |
Protective role of dexmedetomidine in unmethylated CpG-induced inflammation responses in BV2 microglia cells |
title_full |
Protective role of dexmedetomidine in unmethylated CpG-induced inflammation responses in BV2 microglia cells |
title_fullStr |
Protective role of dexmedetomidine in unmethylated CpG-induced inflammation responses in BV2 microglia cells |
title_full_unstemmed |
Protective role of dexmedetomidine in unmethylated CpG-induced inflammation responses in BV2 microglia cells |
title_sort |
protective role of dexmedetomidine in unmethylated cpg-induced inflammation responses in bv2 microglia cells |
publisher |
Termedia Publishing House |
series |
Folia Neuropathologica |
issn |
1641-4640 1509-572X |
publishDate |
2016-12-01 |
description |
Unmethylated CpG DNA, as a stimulatory molecule, has potent pro-inflammatory effects in the central nervous system (CNS). Dexmedetomidine (DEX) has been confirmed to exert anti-inflammatory effects in CNS. Our study was aimed to explore the effects of DEX on tumor necrosis factor-α (TNF-α) expression in unmethylated CpG DNA-challenged microglia. In vivo, after 3 d intracisternal injection of ODN1668, we evaluated the severity of meningitis with or without DEX via pathobiology method and detected the expression of TNF-α from molecular and protein levels. In vitro, we explored whether the ODN1668 could activate microglia to express TNF-α and the inhibition mechanism of DEX. Our results demonstrated that DEX could alleviate the severity of ODN1668-induced meningitis. And while BV2 microglia was stimulated by ODN1668 for different time, TNF-α was increased in mRNA and protein levels but the effect was attenuated by DEX via decreasing phosphorylated AKT and ERK |
topic |
DEX unmethylated CpG DNA microglia |
url |
https://www.termedia.pl/Protective-role-of-dexmedetomidine-in-unmethylated-CpG-induced-inflammation-responses-in-BV2-microglia-cells,20,28979,1,1.html |
work_keys_str_mv |
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