Cultivation of Pichia pastoris carrying the scFv anti LDL (-) antibody fragment. Effect of preculture carbon source

Cultivation of Pichia pastoris carrying the scFv anti LDL (-) antibody fragment. Effect of preculture carbon source

Abstract Antibodies and antibody fragments are nowadays among the most important biotechnological products, and Pichia pastoris is one of the most important vectors to produce them as well as other recombinant proteins. The conditions to effectively cultivate a P. pastoris strain previously genetica...

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Bibliographic Details
Main Authors: Cesar Andres Diaz Arias, Daniela de Araujo Viana Marques, Luciana Pellegrini Malpiedi, Andrea Queiroz Maranhão, Dulcineia Abdalla Saes Parra, Attilio Converti, Adalberto Pessoa Junior
Format: Article
Language:English
Published: Sociedade Brasileira de Microbiologia
Series:Brazilian Journal of Microbiology
Subjects:
Pichia pastoris
scFv antibody fragment
Cryopreservation
Expression
Carbon source
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000300419&lng=en&tlng=en

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