High Dynamic Range Structured Illumination Microscope Based on Multiple Exposures

High Dynamic Range Structured Illumination Microscope Based on Multiple Exposures

Structured illumination microscope (SIM) can double the spatial resolution by using fringed pattern illumination. However, for samples with large intra-scene dynamic ranges, such as clustered objects, SIM fails to reconstruct high-quality images and often exhibits strong artifacts. Herein, we presen...

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Bibliographic Details
Main Authors: Yong Liang, Xiaohu Chen, Zhenglong Sun, Gang Wen, Chong Chen, Libo Wang, Xin Jin, Jie Zhang, Guang Yang, Jing Gao, Hui Li, Simin Li
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-03-01
Series:Frontiers in Physics
Subjects:
super-resolution
structured illumination microscopy
high dynamic range imaging
fusion algorithm
multiple exposures
Online Access:https://www.frontiersin.org/articles/10.3389/fphy.2021.648174/full
Description
Summary:Structured illumination microscope (SIM) can double the spatial resolution by using fringed pattern illumination. However, for samples with large intra-scene dynamic ranges, such as clustered objects, SIM fails to reconstruct high-quality images and often exhibits strong artifacts. Herein, we present a high dynamic range SIM (HDR-SIM) method using a multi-exposure acquisition strategy. With HDR-SIM, individual and clustered microspheres and vesicles with 420 times intensity differences can be visualized in the same scene while the delicate structures of the sample were preserved effectively.
ISSN:2296-424X

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