Feasibility study and direct extraction of endogenous free metallic cations combining hemodialysis and chelating polymer

• Main Authors: Marco Natuzzi, Coralie Grange, Thomas Gréa, Thomas Brichart, Axel Aigle, Denise Bechet, Benoit Hautefeuille, Eloise Thomas, Jean-Yves Ayoub, Jeanne-Marie Bonnet, Vanessa Louzier, Bernard Allaouchiche, Aymeric Couturier, Alexandra Montembault, Paula Nunes de Oliveira, Laurent David, François Lux, Olivier Tillement
• Format: Article
• Language: English
• Published: Nature Publishing Group 2021-10-01
• Series: Scientific Reports
• Online Access: https://doi.org/10.1038/s41598-021-99462-y

Abstract In this article, we report the conception and the use of dialysis-based medical device for the extraction of metals. The medical device is obtained by addition in the dialysate of a functionalized chitosan that can chelate endogenous metals like iron or copper. This water-soluble functionalized chitosan is obtained after controlled reacetylation and grafting of DOTAGA. Due to the high mass of chitosan, the polymer cannot cross through the membrane and the metals are trapped in the dialysate during hemodialysis. Copper extraction has been evaluated in vitro using an hemodialysis protocol. Feasibility study has been performed on healthy sheep showing no acute toxicity througout the entire dialysis procedure and first insights of metallic extraction even on healthy animals.