The potential value of microRNA-4463 in the prognosis evaluation in hepatocellular carcinoma

• Main Authors: Tian Hu, Jincheng Li, Chuhong Zhang, Xiu lv, Sai Li, Sha He, Hanxing Yan, Yixi Tan, Mingsheng Lei, Meiling Wen, Jianhong Zuo
• Format: Article
• Language: English
• Published: Elsevier 2017-06-01
• Series: Genes and Diseases
• Subjects: Hepatocellular carcinoma; malignant tumors; microRNA-4463; microRNA-6087; miR-4463; Prognostic
• Online Access: http://www.sciencedirect.com/science/article/pii/S2352304217300260

The purpose of this study is to measure the expression of microRNA-4463 and microRNA-6087 between normal persons and patients with hepatocellular carcinoma (HCC), and to clarify the meaning of them in the prognosis evaluation in HCC. Forty-five samples from healthy people and patients, who had been diagnosed with hepatocellular carcinoma before any treatment, were collected to study respectively. Real-time PCR was used to detect the expression of miRNA-4463 and miRNA-6087 in the serum of control group and hepatocellular carcinoma patients. The expression of miR-4463 in the serum of HCC patients was significantly higher than that in control group (P < 0.05), and the expression level was independent of gender, tumor size, cell types, stages, alanine aminotransferase (ALT), aspartate aminotransferase (AST), total bilirubin (TBIL) and HBsAg status (P > 0.05). But there was a significant difference of different level of AFP in HCC (P < 0.05), and the difference between the group of AFP lower than 400 ug/l and the control group is statistically significant (P < 0.05). Besides, the survival time had showed a significant difference at the high and low expression levels (P < 0.05). But the expression level of miRNA-6087 was no difference in HCC and control group. The disorder of miRNA-4463 occurred in HCC, even the AFP level doesn't rises. What's more, patients who get the high level of miRNA-4463 seem to have a shorter survival time. And it contributes great to the prognostic evaluation. This is the first study to illustrate the potential significance of miRNA-4463 in the prognosis in HCC.