Lanosterol Synthase Pathway Alleviates Lens Opacity in Age-Related Cortical Cataract
Purpose. Lanosterol synthase (LSS) abnormity contributes to lens opacity in rats, mice, dogs, and human congenital cataract development. This study examined whether LSS pathway has a role in different subtypes of age-related cataract (ARC). Methods. A total of 390 patients with ARC and 88 age-matche...
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doaj-13f22f1a83764501a3291c61e6cf61702020-11-24T21:59:08ZengHindawi LimitedJournal of Ophthalmology2090-004X2090-00582018-01-01201810.1155/2018/41258934125893Lanosterol Synthase Pathway Alleviates Lens Opacity in Age-Related Cortical CataractXinyue Shen0Manhui Zhu1Lihua Kang2Yuanyuan Tu3Lele Li4Rutan Zhang5Bai Qin6Mei Yang7Huaijin Guan8Department of Ophthalmology, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, ChinaDepartment of Ophthalmology, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, ChinaDepartment of Ophthalmology, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, ChinaDepartment of Ophthalmology, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, ChinaDepartment of Ophthalmology, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, ChinaDepartment of Chemistry, Fudan University, Shanghai 200433, ChinaDepartment of Ophthalmology, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, ChinaDepartment of Ophthalmology, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, ChinaDepartment of Ophthalmology, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, ChinaPurpose. Lanosterol synthase (LSS) abnormity contributes to lens opacity in rats, mice, dogs, and human congenital cataract development. This study examined whether LSS pathway has a role in different subtypes of age-related cataract (ARC). Methods. A total of 390 patients with ARC and 88 age-matched non-ARC patients were enrolled in this study. LSS expression was analyzed by western blot and enzyme-linked immunosorbent assay (ELISA). To further examine the function of LSS, we used U18666A, an LSS inhibitor in rat lens culture system. Results. In lens epithelial cells (LECs), LSS expression in LECs increased with opaque degree C II, while it decreased with opaque degree C IV and C V. While in the cortex of age-related cortical cataract (ARCC), LSS expression was negatively related to opaque degree, while lanosterol level was positively correlated to opaque degree. No obvious change in both LSS and lanosterol level was found in either LECs or the cortex of age-related nuclear cataract (ARNC) and age-related posterior subcapsular cataract (ARPSC). In vitro, inhibiting LSS activity induced rat lens opacity and lanosterol effectively delayed the occurrence of lens opacity. Conclusions. This study indicated that LSS and lanosterol were localized in the lens of human ARC, including ARCC, ARNC, and ARPSC. LSS and lanosterol level are only correlated with opaque degree of ARCC. Furthermore, activated LSS pathway in lens is protective for lens transparency in cortical cataract.http://dx.doi.org/10.1155/2018/4125893 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Xinyue Shen Manhui Zhu Lihua Kang Yuanyuan Tu Lele Li Rutan Zhang Bai Qin Mei Yang Huaijin Guan |
spellingShingle |
Xinyue Shen Manhui Zhu Lihua Kang Yuanyuan Tu Lele Li Rutan Zhang Bai Qin Mei Yang Huaijin Guan Lanosterol Synthase Pathway Alleviates Lens Opacity in Age-Related Cortical Cataract Journal of Ophthalmology |
author_facet |
Xinyue Shen Manhui Zhu Lihua Kang Yuanyuan Tu Lele Li Rutan Zhang Bai Qin Mei Yang Huaijin Guan |
author_sort |
Xinyue Shen |
title |
Lanosterol Synthase Pathway Alleviates Lens Opacity in Age-Related Cortical Cataract |
title_short |
Lanosterol Synthase Pathway Alleviates Lens Opacity in Age-Related Cortical Cataract |
title_full |
Lanosterol Synthase Pathway Alleviates Lens Opacity in Age-Related Cortical Cataract |
title_fullStr |
Lanosterol Synthase Pathway Alleviates Lens Opacity in Age-Related Cortical Cataract |
title_full_unstemmed |
Lanosterol Synthase Pathway Alleviates Lens Opacity in Age-Related Cortical Cataract |
title_sort |
lanosterol synthase pathway alleviates lens opacity in age-related cortical cataract |
publisher |
Hindawi Limited |
series |
Journal of Ophthalmology |
issn |
2090-004X 2090-0058 |
publishDate |
2018-01-01 |
description |
Purpose. Lanosterol synthase (LSS) abnormity contributes to lens opacity in rats, mice, dogs, and human congenital cataract development. This study examined whether LSS pathway has a role in different subtypes of age-related cataract (ARC). Methods. A total of 390 patients with ARC and 88 age-matched non-ARC patients were enrolled in this study. LSS expression was analyzed by western blot and enzyme-linked immunosorbent assay (ELISA). To further examine the function of LSS, we used U18666A, an LSS inhibitor in rat lens culture system. Results. In lens epithelial cells (LECs), LSS expression in LECs increased with opaque degree C II, while it decreased with opaque degree C IV and C V. While in the cortex of age-related cortical cataract (ARCC), LSS expression was negatively related to opaque degree, while lanosterol level was positively correlated to opaque degree. No obvious change in both LSS and lanosterol level was found in either LECs or the cortex of age-related nuclear cataract (ARNC) and age-related posterior subcapsular cataract (ARPSC). In vitro, inhibiting LSS activity induced rat lens opacity and lanosterol effectively delayed the occurrence of lens opacity. Conclusions. This study indicated that LSS and lanosterol were localized in the lens of human ARC, including ARCC, ARNC, and ARPSC. LSS and lanosterol level are only correlated with opaque degree of ARCC. Furthermore, activated LSS pathway in lens is protective for lens transparency in cortical cataract. |
url |
http://dx.doi.org/10.1155/2018/4125893 |
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