Elevated Levels of SOX10 in Serum from Vitiligo and Melanoma Patients, Analyzed by Proximity Ligation Assay.

The diagnosis of malignant melanoma currently relies on clinical inspection of the skin surface and on the histopathological status of the excised tumor. The serum marker S100B is used for prognostic estimates at later stages of the disease, but analyses are marred by false positives and inadequate...

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Main Authors: Andries Blokzijl, Lei E Chen, Sigrun M Gustafsdottir, Jimmy Vuu, Gustav Ullenhag, Olle Kämpe, Ulf Landegren, Masood Kamali-Moghaddam, Håkan Hedstrand
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2016-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC4844164?pdf=render
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spelling doaj-13f7560764054bacb3ac9597061989c92020-11-25T01:05:49ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-01114e015421410.1371/journal.pone.0154214Elevated Levels of SOX10 in Serum from Vitiligo and Melanoma Patients, Analyzed by Proximity Ligation Assay.Andries BlokzijlLei E ChenSigrun M GustafsdottirJimmy VuuGustav UllenhagOlle KämpeUlf LandegrenMasood Kamali-MoghaddamHåkan HedstrandThe diagnosis of malignant melanoma currently relies on clinical inspection of the skin surface and on the histopathological status of the excised tumor. The serum marker S100B is used for prognostic estimates at later stages of the disease, but analyses are marred by false positives and inadequate sensitivity in predicting relapsing disorder.To investigate SOX10 as a potential biomarker for melanoma and vitiligo.In this study we have applied proximity ligation assay (PLA) to detect the transcription factor SOX10 as a possible serum marker for melanoma. We studied a cohort of 110 melanoma patients. We further investigated a second cohort of 85 patients with vitiligo, which is a disease that also affects melanocytes.The specificity of the SOX10 assay in serum was high, with only 1% of healthy blood donors being positive. In contrast, elevated serum SOX10 was found with high frequency among vitiligo and melanoma patients. In patients with metastases, lack of SOX10 detection was associated with treatment benefit. In two responding patients, a change from SOX10 positivity to undetectable levels was seen before the response was evident clinically.We show for the first time that SOX10 represents a promising new serum melanoma marker for detection of early stage disease, complementing the established S100B marker. Our findings imply that SOX10 can be used to monitor responses to treatment and to assess if the treatment is of benefit at stages earlier than what is possible radiologically.http://europepmc.org/articles/PMC4844164?pdf=render
collection DOAJ
language English
format Article
sources DOAJ
author Andries Blokzijl
Lei E Chen
Sigrun M Gustafsdottir
Jimmy Vuu
Gustav Ullenhag
Olle Kämpe
Ulf Landegren
Masood Kamali-Moghaddam
Håkan Hedstrand
spellingShingle Andries Blokzijl
Lei E Chen
Sigrun M Gustafsdottir
Jimmy Vuu
Gustav Ullenhag
Olle Kämpe
Ulf Landegren
Masood Kamali-Moghaddam
Håkan Hedstrand
Elevated Levels of SOX10 in Serum from Vitiligo and Melanoma Patients, Analyzed by Proximity Ligation Assay.
PLoS ONE
author_facet Andries Blokzijl
Lei E Chen
Sigrun M Gustafsdottir
Jimmy Vuu
Gustav Ullenhag
Olle Kämpe
Ulf Landegren
Masood Kamali-Moghaddam
Håkan Hedstrand
author_sort Andries Blokzijl
title Elevated Levels of SOX10 in Serum from Vitiligo and Melanoma Patients, Analyzed by Proximity Ligation Assay.
title_short Elevated Levels of SOX10 in Serum from Vitiligo and Melanoma Patients, Analyzed by Proximity Ligation Assay.
title_full Elevated Levels of SOX10 in Serum from Vitiligo and Melanoma Patients, Analyzed by Proximity Ligation Assay.
title_fullStr Elevated Levels of SOX10 in Serum from Vitiligo and Melanoma Patients, Analyzed by Proximity Ligation Assay.
title_full_unstemmed Elevated Levels of SOX10 in Serum from Vitiligo and Melanoma Patients, Analyzed by Proximity Ligation Assay.
title_sort elevated levels of sox10 in serum from vitiligo and melanoma patients, analyzed by proximity ligation assay.
publisher Public Library of Science (PLoS)
series PLoS ONE
issn 1932-6203
publishDate 2016-01-01
description The diagnosis of malignant melanoma currently relies on clinical inspection of the skin surface and on the histopathological status of the excised tumor. The serum marker S100B is used for prognostic estimates at later stages of the disease, but analyses are marred by false positives and inadequate sensitivity in predicting relapsing disorder.To investigate SOX10 as a potential biomarker for melanoma and vitiligo.In this study we have applied proximity ligation assay (PLA) to detect the transcription factor SOX10 as a possible serum marker for melanoma. We studied a cohort of 110 melanoma patients. We further investigated a second cohort of 85 patients with vitiligo, which is a disease that also affects melanocytes.The specificity of the SOX10 assay in serum was high, with only 1% of healthy blood donors being positive. In contrast, elevated serum SOX10 was found with high frequency among vitiligo and melanoma patients. In patients with metastases, lack of SOX10 detection was associated with treatment benefit. In two responding patients, a change from SOX10 positivity to undetectable levels was seen before the response was evident clinically.We show for the first time that SOX10 represents a promising new serum melanoma marker for detection of early stage disease, complementing the established S100B marker. Our findings imply that SOX10 can be used to monitor responses to treatment and to assess if the treatment is of benefit at stages earlier than what is possible radiologically.
url http://europepmc.org/articles/PMC4844164?pdf=render
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