Data on preparation and characterization of an insect odorant receptor based biosensor

• Main Authors: Roshan Khadka, Nihan Aydemir, Colm Carraher, Cyril Hamiaux, Damon Colbert, Jamal Cheema, Jenny Malmström, Andrew Kralicek, Jadranka Travas-Sejdic
• Format: Article
• Language: English
• Published: Elsevier 2018-12-01
• Series: Data in Brief
• Online Access: http://www.sciencedirect.com/science/article/pii/S2352340918314112

Insect Odorant receptors (OrXs) can be used as the recognition element in a biosensor as they demonstrate high levels of sensitivity and selectivity towards volatile organic compounds. Herein, we describe a method to express and purify insect odorant receptors and reconstitute them into artificial lipid bilayers (liposomes). These OrX/liposomes were covalently attached to a gold surface and characterized using quartz crystal microbalance with dissipation monitoring (QCM-D). The interaction of OrX/liposomes immobilized on a gold surface to positive and negative odorants were studied by means of electrochemical impedance spectroscopy (EIS) and QCM-D. The data presented in this article are related to the research article titled “An ultrasensitive electrochemical impedance-based biosensor using insect odorant receptors to detect odorants” [1].