Data on preparation and characterization of an insect odorant receptor based biosensor
Insect Odorant receptors (OrXs) can be used as the recognition element in a biosensor as they demonstrate high levels of sensitivity and selectivity towards volatile organic compounds. Herein, we describe a method to express and purify insect odorant receptors and reconstitute them into artificial l...
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2018-12-01
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doaj-140c2ec1c9b1408fb210e4625f9a5a6b2020-11-25T01:49:40ZengElsevierData in Brief2352-34092018-12-012121422148Data on preparation and characterization of an insect odorant receptor based biosensorRoshan Khadka0Nihan Aydemir1Colm Carraher2Cyril Hamiaux3Damon Colbert4Jamal Cheema5Jenny Malmström6Andrew Kralicek7Jadranka Travas-Sejdic8Polymer Electronic Research Centre, School of Chemical Sciences, University of Auckland, Auckland 1023, New Zealand; MacDiarmid Institute for Advanced Materials and Nanotechnology, Wellington 6140, New ZealandPolymer Electronic Research Centre, School of Chemical Sciences, University of Auckland, Auckland 1023, New Zealand; MacDiarmid Institute for Advanced Materials and Nanotechnology, Wellington 6140, New ZealandThe New Zealand Institute for Plant and Food Research Limited, Private Bag 92169, Auckland 1142, New ZealandThe New Zealand Institute for Plant and Food Research Limited, Private Bag 92169, Auckland 1142, New ZealandThe New Zealand Institute for Plant and Food Research Limited, Private Bag 92169, Auckland 1142, New ZealandPolymer Electronic Research Centre, School of Chemical Sciences, University of Auckland, Auckland 1023, New Zealand; MacDiarmid Institute for Advanced Materials and Nanotechnology, Wellington 6140, New ZealandMacDiarmid Institute for Advanced Materials and Nanotechnology, Wellington 6140, New Zealand; Department of Chemical and Materials Engineering, University of Auckland, Auckland 1023, New ZealandThe New Zealand Institute for Plant and Food Research Limited, Private Bag 92169, Auckland 1142, New Zealand; Corresponding authors.Polymer Electronic Research Centre, School of Chemical Sciences, University of Auckland, Auckland 1023, New Zealand; MacDiarmid Institute for Advanced Materials and Nanotechnology, Wellington 6140, New Zealand; Corresponding authors.Insect Odorant receptors (OrXs) can be used as the recognition element in a biosensor as they demonstrate high levels of sensitivity and selectivity towards volatile organic compounds. Herein, we describe a method to express and purify insect odorant receptors and reconstitute them into artificial lipid bilayers (liposomes). These OrX/liposomes were covalently attached to a gold surface and characterized using quartz crystal microbalance with dissipation monitoring (QCM-D). The interaction of OrX/liposomes immobilized on a gold surface to positive and negative odorants were studied by means of electrochemical impedance spectroscopy (EIS) and QCM-D. The data presented in this article are related to the research article titled “An ultrasensitive electrochemical impedance-based biosensor using insect odorant receptors to detect odorants” [1].http://www.sciencedirect.com/science/article/pii/S2352340918314112 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Roshan Khadka Nihan Aydemir Colm Carraher Cyril Hamiaux Damon Colbert Jamal Cheema Jenny Malmström Andrew Kralicek Jadranka Travas-Sejdic |
spellingShingle |
Roshan Khadka Nihan Aydemir Colm Carraher Cyril Hamiaux Damon Colbert Jamal Cheema Jenny Malmström Andrew Kralicek Jadranka Travas-Sejdic Data on preparation and characterization of an insect odorant receptor based biosensor Data in Brief |
author_facet |
Roshan Khadka Nihan Aydemir Colm Carraher Cyril Hamiaux Damon Colbert Jamal Cheema Jenny Malmström Andrew Kralicek Jadranka Travas-Sejdic |
author_sort |
Roshan Khadka |
title |
Data on preparation and characterization of an insect odorant receptor based biosensor |
title_short |
Data on preparation and characterization of an insect odorant receptor based biosensor |
title_full |
Data on preparation and characterization of an insect odorant receptor based biosensor |
title_fullStr |
Data on preparation and characterization of an insect odorant receptor based biosensor |
title_full_unstemmed |
Data on preparation and characterization of an insect odorant receptor based biosensor |
title_sort |
data on preparation and characterization of an insect odorant receptor based biosensor |
publisher |
Elsevier |
series |
Data in Brief |
issn |
2352-3409 |
publishDate |
2018-12-01 |
description |
Insect Odorant receptors (OrXs) can be used as the recognition element in a biosensor as they demonstrate high levels of sensitivity and selectivity towards volatile organic compounds. Herein, we describe a method to express and purify insect odorant receptors and reconstitute them into artificial lipid bilayers (liposomes). These OrX/liposomes were covalently attached to a gold surface and characterized using quartz crystal microbalance with dissipation monitoring (QCM-D). The interaction of OrX/liposomes immobilized on a gold surface to positive and negative odorants were studied by means of electrochemical impedance spectroscopy (EIS) and QCM-D. The data presented in this article are related to the research article titled “An ultrasensitive electrochemical impedance-based biosensor using insect odorant receptors to detect odorants” [1]. |
url |
http://www.sciencedirect.com/science/article/pii/S2352340918314112 |
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