Ultrasonic Analysis of Peptide- and Antibody-Targeted Microbubble Contrast Agents for Molecular Imaging of αβ-Expressing Cells
The goal of targeted ultrasound contrast agents is to significantly and selectively enhance the detection of a targeted vascular site. In this manuscript, three distinct contrast agents targeted to the α v β 3 integrin are examined. The α v β 3 integrin has been shown to be highly expressed on metas...
Main Authors: | , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Hindawi - SAGE Publishing
2004-04-01
|
Series: | Molecular Imaging |
Online Access: | https://doi.org/10.1162/15353500200403187 |
id |
doaj-14489abcc6bb4f468203d5e22c5f537a |
---|---|
record_format |
Article |
spelling |
doaj-14489abcc6bb4f468203d5e22c5f537a2021-04-02T15:56:31ZengHindawi - SAGE PublishingMolecular Imaging1536-01212004-04-01310.1162/1535350020040318710.1162_15353500200403187Ultrasonic Analysis of Peptide- and Antibody-Targeted Microbubble Contrast Agents for Molecular Imaging of αβ-Expressing CellsPaul A. Dayton0David Pearson1Jarrod Clark2Scott Simon3Patricia A. Schumann4Reena Zutshi5Terry O. Matsunaga6Katherine W. Ferrara7Department of Biomedical Engineering, UC, DavisDepartment of Biomedical Engineering, UC, DavisDepartment of Biomedical Engineering, UC, DavisDepartment of Biomedical Engineering, UC, DavisImaRx Therapeutics, Inc.ImaRx Therapeutics, Inc.ImaRx Therapeutics, Inc.Department of Biomedical Engineering, UC, DavisThe goal of targeted ultrasound contrast agents is to significantly and selectively enhance the detection of a targeted vascular site. In this manuscript, three distinct contrast agents targeted to the α v β 3 integrin are examined. The α v β 3 integrin has been shown to be highly expressed on metastatic tumors and endothelial cells during neovascularization, and its expression has been shown to correlate with tumor grade. Specific adhesion of these contrast agents to α v β 3 -expressing cell monolayers is demonstrated in vitro, and compared with that of nontargeted agents. Acoustic studies illustrate a backscatter amplitude increase from monolayers exposed to the targeted contrast agents of up to 13-fold (22 dB) relative to enhancement due to control bubbles. A linear dependence between the echo amplitude and bubble concentration was observed for bound agents. The decorrelation of the echo from adherent targeted agents is observed over successive pulses as a function of acoustic pressure and bubble density. Frequency–domain analysis demonstrates that adherent targeted bubbles exhibit high-amplitude narrowband echo components, in contrast to the primarily wideband response from free microbubbles. Results suggest that adherent targeted contrast agents are differentiable from free-floating microbubbles, that targeted contrast agents provide higher sensitivity in the detection of angiogenesis, and that conventional ultrasound imaging techniques such as signal subtraction or decorrelation detection can be used to detect integrin-expressing vasculature with sufficient signal-to-noise.https://doi.org/10.1162/15353500200403187 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Paul A. Dayton David Pearson Jarrod Clark Scott Simon Patricia A. Schumann Reena Zutshi Terry O. Matsunaga Katherine W. Ferrara |
spellingShingle |
Paul A. Dayton David Pearson Jarrod Clark Scott Simon Patricia A. Schumann Reena Zutshi Terry O. Matsunaga Katherine W. Ferrara Ultrasonic Analysis of Peptide- and Antibody-Targeted Microbubble Contrast Agents for Molecular Imaging of αβ-Expressing Cells Molecular Imaging |
author_facet |
Paul A. Dayton David Pearson Jarrod Clark Scott Simon Patricia A. Schumann Reena Zutshi Terry O. Matsunaga Katherine W. Ferrara |
author_sort |
Paul A. Dayton |
title |
Ultrasonic Analysis of Peptide- and Antibody-Targeted Microbubble Contrast Agents for Molecular Imaging of αβ-Expressing Cells |
title_short |
Ultrasonic Analysis of Peptide- and Antibody-Targeted Microbubble Contrast Agents for Molecular Imaging of αβ-Expressing Cells |
title_full |
Ultrasonic Analysis of Peptide- and Antibody-Targeted Microbubble Contrast Agents for Molecular Imaging of αβ-Expressing Cells |
title_fullStr |
Ultrasonic Analysis of Peptide- and Antibody-Targeted Microbubble Contrast Agents for Molecular Imaging of αβ-Expressing Cells |
title_full_unstemmed |
Ultrasonic Analysis of Peptide- and Antibody-Targeted Microbubble Contrast Agents for Molecular Imaging of αβ-Expressing Cells |
title_sort |
ultrasonic analysis of peptide- and antibody-targeted microbubble contrast agents for molecular imaging of αβ-expressing cells |
publisher |
Hindawi - SAGE Publishing |
series |
Molecular Imaging |
issn |
1536-0121 |
publishDate |
2004-04-01 |
description |
The goal of targeted ultrasound contrast agents is to significantly and selectively enhance the detection of a targeted vascular site. In this manuscript, three distinct contrast agents targeted to the α v β 3 integrin are examined. The α v β 3 integrin has been shown to be highly expressed on metastatic tumors and endothelial cells during neovascularization, and its expression has been shown to correlate with tumor grade. Specific adhesion of these contrast agents to α v β 3 -expressing cell monolayers is demonstrated in vitro, and compared with that of nontargeted agents. Acoustic studies illustrate a backscatter amplitude increase from monolayers exposed to the targeted contrast agents of up to 13-fold (22 dB) relative to enhancement due to control bubbles. A linear dependence between the echo amplitude and bubble concentration was observed for bound agents. The decorrelation of the echo from adherent targeted agents is observed over successive pulses as a function of acoustic pressure and bubble density. Frequency–domain analysis demonstrates that adherent targeted bubbles exhibit high-amplitude narrowband echo components, in contrast to the primarily wideband response from free microbubbles. Results suggest that adherent targeted contrast agents are differentiable from free-floating microbubbles, that targeted contrast agents provide higher sensitivity in the detection of angiogenesis, and that conventional ultrasound imaging techniques such as signal subtraction or decorrelation detection can be used to detect integrin-expressing vasculature with sufficient signal-to-noise. |
url |
https://doi.org/10.1162/15353500200403187 |
work_keys_str_mv |
AT pauladayton ultrasonicanalysisofpeptideandantibodytargetedmicrobubblecontrastagentsformolecularimagingofabexpressingcells AT davidpearson ultrasonicanalysisofpeptideandantibodytargetedmicrobubblecontrastagentsformolecularimagingofabexpressingcells AT jarrodclark ultrasonicanalysisofpeptideandantibodytargetedmicrobubblecontrastagentsformolecularimagingofabexpressingcells AT scottsimon ultrasonicanalysisofpeptideandantibodytargetedmicrobubblecontrastagentsformolecularimagingofabexpressingcells AT patriciaaschumann ultrasonicanalysisofpeptideandantibodytargetedmicrobubblecontrastagentsformolecularimagingofabexpressingcells AT reenazutshi ultrasonicanalysisofpeptideandantibodytargetedmicrobubblecontrastagentsformolecularimagingofabexpressingcells AT terryomatsunaga ultrasonicanalysisofpeptideandantibodytargetedmicrobubblecontrastagentsformolecularimagingofabexpressingcells AT katherinewferrara ultrasonicanalysisofpeptideandantibodytargetedmicrobubblecontrastagentsformolecularimagingofabexpressingcells |
_version_ |
1721558583502438400 |