In Vitro Antioxidant and Cytotoxic Activities of Arnebia benthamii (Wall ex. G. Don): A Critically Endangered Medicinal Plant of Kashmir Valley
Arnebia benthamii is a major ingredient of the commercial drug available under the name Gaozaban, which has antibacterial, antifungal, anti-inflammatory, and wound-healing properties. In the present study, in vitro antioxidant and anticancer activity of different extracts of Arnebia benthamii were i...
Main Authors: | , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Hindawi Limited
2014-01-01
|
Series: | Oxidative Medicine and Cellular Longevity |
Online Access: | http://dx.doi.org/10.1155/2014/792574 |
id |
doaj-144a268fde9d4ffba27193985f8bd4c8 |
---|---|
record_format |
Article |
spelling |
doaj-144a268fde9d4ffba27193985f8bd4c82020-11-25T02:21:33ZengHindawi LimitedOxidative Medicine and Cellular Longevity1942-09001942-09942014-01-01201410.1155/2014/792574792574In Vitro Antioxidant and Cytotoxic Activities of Arnebia benthamii (Wall ex. G. Don): A Critically Endangered Medicinal Plant of Kashmir ValleyShowkat Ahmad Ganie0Tanveer Ali Dar1Rabia Hamid2Ovais Zargar3Shayaq Ul Abeer4Akbar Masood5Shajrul Amin6Mohammad Afzal Zargar7Clinical Biochemistry, University of Kashmir, Hazratbal, Srinagar 190006, IndiaClinical Biochemistry, University of Kashmir, Hazratbal, Srinagar 190006, IndiaDepartment of Biochemistry, University of Kashmir, Hazratbal, Srinagar 190006, IndiaClinical Biochemistry, University of Kashmir, Hazratbal, Srinagar 190006, IndiaDepartment of Biotechnology, University of Kashmir, Srinagar, Hazratbal, Srinagar 190006, IndiaDepartment of Biochemistry, University of Kashmir, Hazratbal, Srinagar 190006, IndiaDepartment of Biochemistry, University of Kashmir, Hazratbal, Srinagar 190006, IndiaClinical Biochemistry, University of Kashmir, Hazratbal, Srinagar 190006, IndiaArnebia benthamii is a major ingredient of the commercial drug available under the name Gaozaban, which has antibacterial, antifungal, anti-inflammatory, and wound-healing properties. In the present study, in vitro antioxidant and anticancer activity of different extracts of Arnebia benthamii were investigated. Antioxidant potential of plant extracts was evaluated by means of total phenolics, DPPH, reducing power, microsomal lipid peroxidation, and hydroxyl radical scavenging activity. The highest phenolic content (TPC) of 780 mg GAE/g was observed in ethyl acetate, while the lowest TPC of 462 mg GAE/g was achieved in aqueous extract. At concentration of 700 µg/mL, DPPH radical scavenging activity was found to be highest in ethyl acetate extract (87.99%) and lowest in aqueous extract (73%). The reducing power of extracts increased in a concentration dependent manner. We also observed its inhibition on Fe2+/ascorbic acid-induced lipid peroxidation (LPO) on rat liver microsomes in vitro. In addition, Arnebia benthamii extracts exhibited antioxidant effects on Calf thymus DNA damage induced by Fenton reaction. Cytotoxicity of the extracts (10–100 µg/mL) was tested on five human cancer cell lines (lung, prostate, leukemia, colon, and pancreatic cell lines) using the Sulphorhodamine B assay.http://dx.doi.org/10.1155/2014/792574 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Showkat Ahmad Ganie Tanveer Ali Dar Rabia Hamid Ovais Zargar Shayaq Ul Abeer Akbar Masood Shajrul Amin Mohammad Afzal Zargar |
spellingShingle |
Showkat Ahmad Ganie Tanveer Ali Dar Rabia Hamid Ovais Zargar Shayaq Ul Abeer Akbar Masood Shajrul Amin Mohammad Afzal Zargar In Vitro Antioxidant and Cytotoxic Activities of Arnebia benthamii (Wall ex. G. Don): A Critically Endangered Medicinal Plant of Kashmir Valley Oxidative Medicine and Cellular Longevity |
author_facet |
Showkat Ahmad Ganie Tanveer Ali Dar Rabia Hamid Ovais Zargar Shayaq Ul Abeer Akbar Masood Shajrul Amin Mohammad Afzal Zargar |
author_sort |
Showkat Ahmad Ganie |
title |
In Vitro Antioxidant and Cytotoxic Activities of Arnebia benthamii (Wall ex. G. Don): A Critically Endangered Medicinal Plant of Kashmir Valley |
title_short |
In Vitro Antioxidant and Cytotoxic Activities of Arnebia benthamii (Wall ex. G. Don): A Critically Endangered Medicinal Plant of Kashmir Valley |
title_full |
In Vitro Antioxidant and Cytotoxic Activities of Arnebia benthamii (Wall ex. G. Don): A Critically Endangered Medicinal Plant of Kashmir Valley |
title_fullStr |
In Vitro Antioxidant and Cytotoxic Activities of Arnebia benthamii (Wall ex. G. Don): A Critically Endangered Medicinal Plant of Kashmir Valley |
title_full_unstemmed |
In Vitro Antioxidant and Cytotoxic Activities of Arnebia benthamii (Wall ex. G. Don): A Critically Endangered Medicinal Plant of Kashmir Valley |
title_sort |
in vitro antioxidant and cytotoxic activities of arnebia benthamii (wall ex. g. don): a critically endangered medicinal plant of kashmir valley |
publisher |
Hindawi Limited |
series |
Oxidative Medicine and Cellular Longevity |
issn |
1942-0900 1942-0994 |
publishDate |
2014-01-01 |
description |
Arnebia benthamii is a major ingredient of the commercial drug available under the name Gaozaban, which has antibacterial, antifungal, anti-inflammatory, and wound-healing properties. In the present study, in vitro antioxidant and anticancer activity of different extracts of Arnebia benthamii were investigated. Antioxidant potential of plant extracts was evaluated by means of total phenolics, DPPH, reducing power, microsomal lipid peroxidation, and hydroxyl radical scavenging activity. The highest phenolic content (TPC) of 780 mg GAE/g was observed in ethyl acetate, while the lowest TPC of 462 mg GAE/g was achieved in aqueous extract. At concentration of 700 µg/mL, DPPH radical scavenging activity was found to be highest in ethyl acetate extract (87.99%) and lowest in aqueous extract (73%). The reducing power of extracts increased in a concentration dependent manner. We also observed its inhibition on Fe2+/ascorbic acid-induced lipid peroxidation (LPO) on rat liver microsomes in vitro. In addition, Arnebia benthamii extracts exhibited antioxidant effects on Calf thymus DNA damage induced by Fenton reaction. Cytotoxicity of the extracts (10–100 µg/mL) was tested on five human cancer cell lines (lung, prostate, leukemia, colon, and pancreatic cell lines) using the Sulphorhodamine B assay. |
url |
http://dx.doi.org/10.1155/2014/792574 |
work_keys_str_mv |
AT showkatahmadganie invitroantioxidantandcytotoxicactivitiesofarnebiabenthamiiwallexgdonacriticallyendangeredmedicinalplantofkashmirvalley AT tanveeralidar invitroantioxidantandcytotoxicactivitiesofarnebiabenthamiiwallexgdonacriticallyendangeredmedicinalplantofkashmirvalley AT rabiahamid invitroantioxidantandcytotoxicactivitiesofarnebiabenthamiiwallexgdonacriticallyendangeredmedicinalplantofkashmirvalley AT ovaiszargar invitroantioxidantandcytotoxicactivitiesofarnebiabenthamiiwallexgdonacriticallyendangeredmedicinalplantofkashmirvalley AT shayaqulabeer invitroantioxidantandcytotoxicactivitiesofarnebiabenthamiiwallexgdonacriticallyendangeredmedicinalplantofkashmirvalley AT akbarmasood invitroantioxidantandcytotoxicactivitiesofarnebiabenthamiiwallexgdonacriticallyendangeredmedicinalplantofkashmirvalley AT shajrulamin invitroantioxidantandcytotoxicactivitiesofarnebiabenthamiiwallexgdonacriticallyendangeredmedicinalplantofkashmirvalley AT mohammadafzalzargar invitroantioxidantandcytotoxicactivitiesofarnebiabenthamiiwallexgdonacriticallyendangeredmedicinalplantofkashmirvalley |
_version_ |
1724865365304934400 |