Exogenous Serotonin Improves Salt Tolerance in Rapeseed (<i>Brassica napus</i> L.) Seedlings
Serotonin is a well-known agent that plays various roles in animals, and is little known in plants. In this study, the effect of exogenous serotonin was tested on <i>Brassica napus L</i>. (rapeseed) under salt stress. The results revealed that exogenous application of 200 µmol/L serotoni...
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doaj-144be497990b4f1d983c2ca26342bdae2021-04-02T20:15:46ZengMDPI AGAgronomy2073-43952021-02-011140040010.3390/agronomy11020400Exogenous Serotonin Improves Salt Tolerance in Rapeseed (<i>Brassica napus</i> L.) SeedlingsYue Liu0Xiaoyu Ding1Yan Lv2Yong Cheng3Chunsheng Li4Lei Yan5Shanshan Tian6Xiling Zou7Key Lab of Biology and Genetic Improvement of Oil Crops of Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, ChinaKey Lab of Biology and Genetic Improvement of Oil Crops of Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, ChinaKey Lab of Biology and Genetic Improvement of Oil Crops of Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, ChinaKey Lab of Biology and Genetic Improvement of Oil Crops of Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, ChinaHubei Institute of New Socialist Countryside Development, Hubei Engineering University, Xiaogan 432000, ChinaKey Lab of Biology and Genetic Improvement of Oil Crops of Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, ChinaKey Lab of Biology and Genetic Improvement of Oil Crops of Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, ChinaKey Lab of Biology and Genetic Improvement of Oil Crops of Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, ChinaSerotonin is a well-known agent that plays various roles in animals, and is little known in plants. In this study, the effect of exogenous serotonin was tested on <i>Brassica napus L</i>. (rapeseed) under salt stress. The results revealed that exogenous application of 200 µmol/L serotonin had the best protection under salinity. Exogenous serotonin effectively alleviated the growth inhibition of seedlings caused by salinity, and significantly promoted the accumulation of the fresh and dry weights of roots and shoots. Besides, although the H<sub>2</sub>O<sub>2</sub> and malondialdehyde (MDA) contents were raised under salinity, they were reduced by exogenous serotonin. The chlorophyll content was decreased under salinity, and was increased by exogenous serotonin. Under salinity, serotonin effectively activated antioxidant enzyme system through improving the catalase (CAT), superoxide dismutase (SOD), and peroxidase (POD) activities, and the expression of <i>POD7</i>, <i>CAT3 </i>and <i>Cu-SOD </i>genes was also up-regulated. The results also revealed exogenous serotonin increased the solute content by promoting the accumulation of soluble sugar and protein. In conclusion, salinity caused a toxicity to seedlings through oxidative damage to chlorophyll and cell membrane integrity, and serotonin possessed the ability of scavenging reactive oxygen species, osmotic pressure regulation and promoting growth, thus alleviating salinity of rape seedlings.https://www.mdpi.com/2073-4395/11/2/400serotonin<i>Brassica napus </i>L. (rapeseed)salinityseedling |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yue Liu Xiaoyu Ding Yan Lv Yong Cheng Chunsheng Li Lei Yan Shanshan Tian Xiling Zou |
spellingShingle |
Yue Liu Xiaoyu Ding Yan Lv Yong Cheng Chunsheng Li Lei Yan Shanshan Tian Xiling Zou Exogenous Serotonin Improves Salt Tolerance in Rapeseed (<i>Brassica napus</i> L.) Seedlings Agronomy serotonin <i>Brassica napus </i>L. (rapeseed) salinity seedling |
author_facet |
Yue Liu Xiaoyu Ding Yan Lv Yong Cheng Chunsheng Li Lei Yan Shanshan Tian Xiling Zou |
author_sort |
Yue Liu |
title |
Exogenous Serotonin Improves Salt Tolerance in Rapeseed (<i>Brassica napus</i> L.) Seedlings |
title_short |
Exogenous Serotonin Improves Salt Tolerance in Rapeseed (<i>Brassica napus</i> L.) Seedlings |
title_full |
Exogenous Serotonin Improves Salt Tolerance in Rapeseed (<i>Brassica napus</i> L.) Seedlings |
title_fullStr |
Exogenous Serotonin Improves Salt Tolerance in Rapeseed (<i>Brassica napus</i> L.) Seedlings |
title_full_unstemmed |
Exogenous Serotonin Improves Salt Tolerance in Rapeseed (<i>Brassica napus</i> L.) Seedlings |
title_sort |
exogenous serotonin improves salt tolerance in rapeseed (<i>brassica napus</i> l.) seedlings |
publisher |
MDPI AG |
series |
Agronomy |
issn |
2073-4395 |
publishDate |
2021-02-01 |
description |
Serotonin is a well-known agent that plays various roles in animals, and is little known in plants. In this study, the effect of exogenous serotonin was tested on <i>Brassica napus L</i>. (rapeseed) under salt stress. The results revealed that exogenous application of 200 µmol/L serotonin had the best protection under salinity. Exogenous serotonin effectively alleviated the growth inhibition of seedlings caused by salinity, and significantly promoted the accumulation of the fresh and dry weights of roots and shoots. Besides, although the H<sub>2</sub>O<sub>2</sub> and malondialdehyde (MDA) contents were raised under salinity, they were reduced by exogenous serotonin. The chlorophyll content was decreased under salinity, and was increased by exogenous serotonin. Under salinity, serotonin effectively activated antioxidant enzyme system through improving the catalase (CAT), superoxide dismutase (SOD), and peroxidase (POD) activities, and the expression of <i>POD7</i>, <i>CAT3 </i>and <i>Cu-SOD </i>genes was also up-regulated. The results also revealed exogenous serotonin increased the solute content by promoting the accumulation of soluble sugar and protein. In conclusion, salinity caused a toxicity to seedlings through oxidative damage to chlorophyll and cell membrane integrity, and serotonin possessed the ability of scavenging reactive oxygen species, osmotic pressure regulation and promoting growth, thus alleviating salinity of rape seedlings. |
topic |
serotonin <i>Brassica napus </i>L. (rapeseed) salinity seedling |
url |
https://www.mdpi.com/2073-4395/11/2/400 |
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