Single-Cell RNA Sequencing Efficiently Predicts Transcription Factor Targets in Plants
Discovering transcription factor (TF) targets is necessary for the study of regulatory pathways, but it is hampered in plants by the lack of highly efficient predictive technology. This study is the first to establish a simple system for predicting TF targets in rice (Oryza sativa) leaf cells based...
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Frontiers Media S.A.
2020-12-01
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Series: | Frontiers in Plant Science |
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Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2020.603302/full |
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author |
Yunjie Xie Yunjie Xie Yunjie Xie Yunjie Xie Yunjie Xie Yunjie Xie Yunjie Xie Yunjie Xie Shenfei Jiang Shenfei Jiang Shenfei Jiang Shenfei Jiang Shenfei Jiang Shenfei Jiang Shenfei Jiang Shenfei Jiang Lele Li Lele Li Lele Li Lele Li Lele Li Lele Li Lele Li Lele Li Xiangzhen Yu Xiangzhen Yu Xiangzhen Yu Xiangzhen Yu Xiangzhen Yu Xiangzhen Yu Xiangzhen Yu Xiangzhen Yu Yupeng Wang Yupeng Wang Yupeng Wang Yupeng Wang Yupeng Wang Yupeng Wang Yupeng Wang Yupeng Wang Cuiqin Luo Cuiqin Luo Cuiqin Luo Cuiqin Luo Cuiqin Luo Cuiqin Luo Cuiqin Luo Cuiqin Luo Qiuhua Cai Qiuhua Cai Qiuhua Cai Qiuhua Cai Qiuhua Cai Qiuhua Cai Qiuhua Cai Wei He Wei He Wei He Wei He Wei He Wei He Wei He Hongguang Xie Hongguang Xie Hongguang Xie Hongguang Xie Hongguang Xie Hongguang Xie Hongguang Xie Yanmei Zheng Yanmei Zheng Yanmei Zheng Yanmei Zheng Yanmei Zheng Yanmei Zheng Yanmei Zheng Yanmei Zheng Huaan Xie Huaan Xie Huaan Xie Huaan Xie Huaan Xie Huaan Xie Huaan Xie Huaan Xie Huaan Xie Jianfu Zhang Jianfu Zhang Jianfu Zhang Jianfu Zhang Jianfu Zhang Jianfu Zhang Jianfu Zhang Jianfu Zhang Jianfu Zhang |
spellingShingle |
Yunjie Xie Yunjie Xie Yunjie Xie Yunjie Xie Yunjie Xie Yunjie Xie Yunjie Xie Yunjie Xie Shenfei Jiang Shenfei Jiang Shenfei Jiang Shenfei Jiang Shenfei Jiang Shenfei Jiang Shenfei Jiang Shenfei Jiang Lele Li Lele Li Lele Li Lele Li Lele Li Lele Li Lele Li Lele Li Xiangzhen Yu Xiangzhen Yu Xiangzhen Yu Xiangzhen Yu Xiangzhen Yu Xiangzhen Yu Xiangzhen Yu Xiangzhen Yu Yupeng Wang Yupeng Wang Yupeng Wang Yupeng Wang Yupeng Wang Yupeng Wang Yupeng Wang Yupeng Wang Cuiqin Luo Cuiqin Luo Cuiqin Luo Cuiqin Luo Cuiqin Luo Cuiqin Luo Cuiqin Luo Cuiqin Luo Qiuhua Cai Qiuhua Cai Qiuhua Cai Qiuhua Cai Qiuhua Cai Qiuhua Cai Qiuhua Cai Wei He Wei He Wei He Wei He Wei He Wei He Wei He Hongguang Xie Hongguang Xie Hongguang Xie Hongguang Xie Hongguang Xie Hongguang Xie Hongguang Xie Yanmei Zheng Yanmei Zheng Yanmei Zheng Yanmei Zheng Yanmei Zheng Yanmei Zheng Yanmei Zheng Yanmei Zheng Huaan Xie Huaan Xie Huaan Xie Huaan Xie Huaan Xie Huaan Xie Huaan Xie Huaan Xie Huaan Xie Jianfu Zhang Jianfu Zhang Jianfu Zhang Jianfu Zhang Jianfu Zhang Jianfu Zhang Jianfu Zhang Jianfu Zhang Jianfu Zhang Single-Cell RNA Sequencing Efficiently Predicts Transcription Factor Targets in Plants Frontiers in Plant Science transcription factor TF targets OsNAC78 expression trajectory scRNA-seq |
author_facet |
Yunjie Xie Yunjie Xie Yunjie Xie Yunjie Xie Yunjie Xie Yunjie Xie Yunjie Xie Yunjie Xie Shenfei Jiang Shenfei Jiang Shenfei Jiang Shenfei Jiang Shenfei Jiang Shenfei Jiang Shenfei Jiang Shenfei Jiang Lele Li Lele Li Lele Li Lele Li Lele Li Lele Li Lele Li Lele Li Xiangzhen Yu Xiangzhen Yu Xiangzhen Yu Xiangzhen Yu Xiangzhen Yu Xiangzhen Yu Xiangzhen Yu Xiangzhen Yu Yupeng Wang Yupeng Wang Yupeng Wang Yupeng Wang Yupeng Wang Yupeng Wang Yupeng Wang Yupeng Wang Cuiqin Luo Cuiqin Luo Cuiqin Luo Cuiqin Luo Cuiqin Luo Cuiqin Luo Cuiqin Luo Cuiqin Luo Qiuhua Cai Qiuhua Cai Qiuhua Cai Qiuhua Cai Qiuhua Cai Qiuhua Cai Qiuhua Cai Wei He Wei He Wei He Wei He Wei He Wei He Wei He Hongguang Xie Hongguang Xie Hongguang Xie Hongguang Xie Hongguang Xie Hongguang Xie Hongguang Xie Yanmei Zheng Yanmei Zheng Yanmei Zheng Yanmei Zheng Yanmei Zheng Yanmei Zheng Yanmei Zheng Yanmei Zheng Huaan Xie Huaan Xie Huaan Xie Huaan Xie Huaan Xie Huaan Xie Huaan Xie Huaan Xie Huaan Xie Jianfu Zhang Jianfu Zhang Jianfu Zhang Jianfu Zhang Jianfu Zhang Jianfu Zhang Jianfu Zhang Jianfu Zhang Jianfu Zhang |
author_sort |
Yunjie Xie |
title |
Single-Cell RNA Sequencing Efficiently Predicts Transcription Factor Targets in Plants |
title_short |
Single-Cell RNA Sequencing Efficiently Predicts Transcription Factor Targets in Plants |
title_full |
Single-Cell RNA Sequencing Efficiently Predicts Transcription Factor Targets in Plants |
title_fullStr |
Single-Cell RNA Sequencing Efficiently Predicts Transcription Factor Targets in Plants |
title_full_unstemmed |
Single-Cell RNA Sequencing Efficiently Predicts Transcription Factor Targets in Plants |
title_sort |
single-cell rna sequencing efficiently predicts transcription factor targets in plants |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Plant Science |
issn |
1664-462X |
publishDate |
2020-12-01 |
description |
Discovering transcription factor (TF) targets is necessary for the study of regulatory pathways, but it is hampered in plants by the lack of highly efficient predictive technology. This study is the first to establish a simple system for predicting TF targets in rice (Oryza sativa) leaf cells based on 10 × Genomics’ single-cell RNA sequencing method. We effectively utilized the transient expression system to create the differential expression of a TF (OsNAC78) in each cell and sequenced all single cell transcriptomes. In total, 35 candidate targets having strong correlations with OsNAC78 expression were captured using expression profiles. Likewise, 78 potential differentially expressed genes were identified between clusters having the lowest and highest expression levels of OsNAC78. A gene overlapping analysis identified 19 genes as final candidate targets, and various assays indicated that Os01g0934800 and Os01g0949900 were OsNAC78 targets. Additionally, the cell profiles showed extremely similar expression trajectories between OsNAC78 and the two targets. The data presented here provide a high-resolution insight into predicting TF targets and offer a new application for single-cell RNA sequencing in plants. |
topic |
transcription factor TF targets OsNAC78 expression trajectory scRNA-seq |
url |
https://www.frontiersin.org/articles/10.3389/fpls.2020.603302/full |
work_keys_str_mv |
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doaj-1479e6c86d3549f8ab391961a56052152020-12-08T05:30:52ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2020-12-011110.3389/fpls.2020.603302603302Single-Cell RNA Sequencing Efficiently Predicts Transcription Factor Targets in PlantsYunjie Xie0Yunjie Xie1Yunjie Xie2Yunjie Xie3Yunjie Xie4Yunjie Xie5Yunjie Xie6Yunjie Xie7Shenfei Jiang8Shenfei Jiang9Shenfei Jiang10Shenfei Jiang11Shenfei Jiang12Shenfei Jiang13Shenfei Jiang14Shenfei Jiang15Lele Li16Lele Li17Lele Li18Lele Li19Lele Li20Lele Li21Lele Li22Lele Li23Xiangzhen Yu24Xiangzhen Yu25Xiangzhen Yu26Xiangzhen Yu27Xiangzhen Yu28Xiangzhen Yu29Xiangzhen Yu30Xiangzhen Yu31Yupeng Wang32Yupeng Wang33Yupeng Wang34Yupeng Wang35Yupeng Wang36Yupeng Wang37Yupeng Wang38Yupeng Wang39Cuiqin Luo40Cuiqin Luo41Cuiqin Luo42Cuiqin Luo43Cuiqin Luo44Cuiqin Luo45Cuiqin Luo46Cuiqin Luo47Qiuhua Cai48Qiuhua Cai49Qiuhua Cai50Qiuhua Cai51Qiuhua Cai52Qiuhua Cai53Qiuhua Cai54Wei He55Wei He56Wei He57Wei He58Wei He59Wei He60Wei He61Hongguang Xie62Hongguang Xie63Hongguang Xie64Hongguang Xie65Hongguang Xie66Hongguang Xie67Hongguang Xie68Yanmei Zheng69Yanmei Zheng70Yanmei Zheng71Yanmei Zheng72Yanmei Zheng73Yanmei Zheng74Yanmei Zheng75Yanmei Zheng76Huaan Xie77Huaan Xie78Huaan Xie79Huaan Xie80Huaan Xie81Huaan Xie82Huaan Xie83Huaan Xie84Huaan Xie85Jianfu Zhang86Jianfu Zhang87Jianfu Zhang88Jianfu Zhang89Jianfu Zhang90Jianfu Zhang91Jianfu Zhang92Jianfu Zhang93Jianfu Zhang94College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, ChinaRice Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fuzhou, ChinaKey Laboratory of Germplasm Innovation and Molecular Breeding of Hybrid Rice for South China, Ministry of Agriculture and Rural Affairs, Fuzhou, ChinaIncubator of National Key Laboratory of Germplasm Innovation and Molecular Breeding Between Fujian and Ministry of Sciences and Technology, Fuzhou, ChinaFuzhou Branch, National Rice Improvement Center of China, Fuzhou, ChinaFujian Engineering Laboratory of Crop Molecular Breeding, Fuzhou, ChinaBase of South China, State Key Laboratory of Hybrid Rice, Fuzhou, ChinaCollege of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, ChinaRice Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fuzhou, ChinaKey Laboratory of Germplasm Innovation and Molecular Breeding of Hybrid Rice for South China, Ministry of Agriculture and Rural Affairs, Fuzhou, ChinaIncubator of National Key Laboratory of Germplasm Innovation and Molecular Breeding Between Fujian and Ministry of Sciences and Technology, Fuzhou, ChinaFuzhou Branch, National Rice Improvement Center of China, Fuzhou, ChinaFujian Engineering Laboratory of Crop Molecular Breeding, Fuzhou, ChinaBase of South China, State Key Laboratory of Hybrid Rice, Fuzhou, ChinaRice Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fuzhou, ChinaKey Laboratory of Germplasm Innovation and Molecular Breeding of Hybrid Rice for South China, Ministry of Agriculture and Rural Affairs, Fuzhou, ChinaIncubator of National Key Laboratory of Germplasm Innovation and Molecular Breeding Between Fujian and Ministry of Sciences and Technology, Fuzhou, ChinaFuzhou Branch, National Rice Improvement Center of China, Fuzhou, ChinaFujian Engineering Laboratory of Crop Molecular Breeding, Fuzhou, ChinaBase of South China, State Key Laboratory of Hybrid Rice, Fuzhou, ChinaCollege of Agronomy, Fujian Agriculture and Forestry University, Fuzhou, ChinaCollege of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, ChinaRice Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fuzhou, ChinaKey Laboratory of Germplasm Innovation and Molecular Breeding of Hybrid Rice for South China, Ministry of Agriculture and Rural Affairs, Fuzhou, ChinaIncubator of National Key Laboratory of Germplasm Innovation and Molecular Breeding Between Fujian and Ministry of Sciences and Technology, Fuzhou, ChinaFuzhou Branch, National Rice Improvement Center of China, Fuzhou, ChinaFujian Engineering Laboratory of Crop Molecular Breeding, Fuzhou, ChinaBase of South China, State Key Laboratory of Hybrid Rice, Fuzhou, ChinaCollege of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, ChinaRice Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fuzhou, ChinaKey Laboratory of Germplasm Innovation and Molecular Breeding of Hybrid Rice for South China, Ministry of Agriculture and Rural Affairs, Fuzhou, ChinaIncubator of National Key Laboratory of Germplasm Innovation and Molecular Breeding Between Fujian and Ministry of Sciences and Technology, Fuzhou, ChinaFuzhou Branch, National Rice Improvement Center of China, Fuzhou, ChinaFujian Engineering Laboratory of Crop Molecular Breeding, Fuzhou, ChinaBase of South China, State Key Laboratory of Hybrid Rice, Fuzhou, ChinaCollege of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, ChinaRice Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fuzhou, ChinaKey Laboratory of Germplasm Innovation and Molecular Breeding of Hybrid Rice for South China, Ministry of Agriculture and Rural Affairs, Fuzhou, ChinaIncubator of National Key Laboratory of Germplasm Innovation and Molecular Breeding Between Fujian and Ministry of Sciences and Technology, Fuzhou, ChinaFuzhou Branch, National Rice Improvement Center of China, Fuzhou, ChinaFujian Engineering Laboratory of Crop Molecular Breeding, Fuzhou, ChinaBase of South China, State Key Laboratory of Hybrid Rice, Fuzhou, ChinaRice Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fuzhou, ChinaKey Laboratory of Germplasm Innovation and Molecular Breeding of Hybrid Rice for South China, Ministry of Agriculture and Rural Affairs, Fuzhou, ChinaIncubator of National Key Laboratory of Germplasm Innovation and Molecular Breeding Between Fujian and Ministry of Sciences and Technology, Fuzhou, ChinaFuzhou Branch, National Rice Improvement Center of China, Fuzhou, ChinaFujian Engineering Laboratory of Crop Molecular Breeding, Fuzhou, ChinaBase of South China, State Key Laboratory of Hybrid Rice, Fuzhou, ChinaRice Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fuzhou, ChinaKey Laboratory of Germplasm Innovation and Molecular Breeding of Hybrid Rice for South China, Ministry of Agriculture and Rural Affairs, Fuzhou, ChinaIncubator of National Key Laboratory of Germplasm Innovation and Molecular Breeding Between Fujian and Ministry of Sciences and Technology, Fuzhou, ChinaFuzhou Branch, National Rice Improvement Center of China, Fuzhou, ChinaFujian Engineering Laboratory of Crop Molecular Breeding, Fuzhou, ChinaBase of South China, State Key Laboratory of Hybrid Rice, Fuzhou, ChinaRice Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fuzhou, ChinaKey Laboratory of Germplasm Innovation and Molecular Breeding of Hybrid Rice for South China, Ministry of Agriculture and Rural Affairs, Fuzhou, ChinaIncubator of National Key Laboratory of Germplasm Innovation and Molecular Breeding Between Fujian and Ministry of Sciences and Technology, Fuzhou, ChinaFuzhou Branch, National Rice Improvement Center of China, Fuzhou, ChinaFujian Engineering Laboratory of Crop Molecular Breeding, Fuzhou, ChinaBase of South China, State Key Laboratory of Hybrid Rice, Fuzhou, ChinaCollege of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, ChinaRice Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fuzhou, ChinaKey Laboratory of Germplasm Innovation and Molecular Breeding of Hybrid Rice for South China, Ministry of Agriculture and Rural Affairs, Fuzhou, ChinaIncubator of National Key Laboratory of Germplasm Innovation and Molecular Breeding Between Fujian and Ministry of Sciences and Technology, Fuzhou, ChinaFuzhou Branch, National Rice Improvement Center of China, Fuzhou, ChinaFujian Engineering Laboratory of Crop Molecular Breeding, Fuzhou, ChinaBase of South China, State Key Laboratory of Hybrid Rice, Fuzhou, ChinaCollege of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, ChinaRice Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fuzhou, ChinaKey Laboratory of Germplasm Innovation and Molecular Breeding of Hybrid Rice for South China, Ministry of Agriculture and Rural Affairs, Fuzhou, ChinaIncubator of National Key Laboratory of Germplasm Innovation and Molecular Breeding Between Fujian and Ministry of Sciences and Technology, Fuzhou, ChinaFuzhou Branch, National Rice Improvement Center of China, Fuzhou, ChinaFujian Engineering Laboratory of Crop Molecular Breeding, Fuzhou, ChinaBase of South China, State Key Laboratory of Hybrid Rice, Fuzhou, ChinaCollege of Agronomy, Fujian Agriculture and Forestry University, Fuzhou, ChinaCollege of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, ChinaRice Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fuzhou, ChinaKey Laboratory of Germplasm Innovation and Molecular Breeding of Hybrid Rice for South China, Ministry of Agriculture and Rural Affairs, Fuzhou, ChinaIncubator of National Key Laboratory of Germplasm Innovation and Molecular Breeding Between Fujian and Ministry of Sciences and Technology, Fuzhou, ChinaFuzhou Branch, National Rice Improvement Center of China, Fuzhou, ChinaFujian Engineering Laboratory of Crop Molecular Breeding, Fuzhou, ChinaBase of South China, State Key Laboratory of Hybrid Rice, Fuzhou, ChinaCollege of Agronomy, Fujian Agriculture and Forestry University, Fuzhou, ChinaDiscovering transcription factor (TF) targets is necessary for the study of regulatory pathways, but it is hampered in plants by the lack of highly efficient predictive technology. This study is the first to establish a simple system for predicting TF targets in rice (Oryza sativa) leaf cells based on 10 × Genomics’ single-cell RNA sequencing method. We effectively utilized the transient expression system to create the differential expression of a TF (OsNAC78) in each cell and sequenced all single cell transcriptomes. In total, 35 candidate targets having strong correlations with OsNAC78 expression were captured using expression profiles. Likewise, 78 potential differentially expressed genes were identified between clusters having the lowest and highest expression levels of OsNAC78. A gene overlapping analysis identified 19 genes as final candidate targets, and various assays indicated that Os01g0934800 and Os01g0949900 were OsNAC78 targets. Additionally, the cell profiles showed extremely similar expression trajectories between OsNAC78 and the two targets. The data presented here provide a high-resolution insight into predicting TF targets and offer a new application for single-cell RNA sequencing in plants.https://www.frontiersin.org/articles/10.3389/fpls.2020.603302/fulltranscription factorTF targetsOsNAC78expression trajectoryscRNA-seq |