Use of Busulfan to Condition Mice for Bone Marrow Transplantation

Use of Busulfan to Condition Mice for Bone Marrow Transplantation

Summary: Myeloablative gamma irradiation has traditionally been used to condition mice prior to bone marrow transplantation. However, irradiation induces high levels of inflammation that may alter patterns of reconstitution. In addition, gamma irradiators are being removed from many facilities for s...

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Main Authors: Encarnacion Montecino-Rodriguez, Kenneth Dorshkind
Format: Article
Language:English
Published: Elsevier 2020-12-01
Series:STAR Protocols
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166720301465
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spelling doaj-14afbef90a42436dba5909c68dac91062020-12-21T04:48:56ZengElsevierSTAR Protocols2666-16672020-12-0113100159Use of Busulfan to Condition Mice for Bone Marrow TransplantationEncarnacion Montecino-Rodriguez0Kenneth Dorshkind1Department of Pathology and Laboratory Medicine, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USADepartment of Pathology and Laboratory Medicine, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USA; Corresponding authorSummary: Myeloablative gamma irradiation has traditionally been used to condition mice prior to bone marrow transplantation. However, irradiation induces high levels of inflammation that may alter patterns of reconstitution. In addition, gamma irradiators are being removed from many facilities for security reasons. Alternative conditioning regimens are thus needed. Here, we describe a protocol for the use of busulfan to condition mice for bone marrow transplantation and several of the variables to consider for effective implementation.For complete details on the use and execution of this protocol, please refer to Montecino-Rodriguez et al. (2019).http://www.sciencedirect.com/science/article/pii/S2666166720301465
collection DOAJ
language English
format Article
sources DOAJ
author Encarnacion Montecino-Rodriguez
Kenneth Dorshkind
spellingShingle Encarnacion Montecino-Rodriguez
Kenneth Dorshkind
Use of Busulfan to Condition Mice for Bone Marrow Transplantation
STAR Protocols
author_facet Encarnacion Montecino-Rodriguez
Kenneth Dorshkind
author_sort Encarnacion Montecino-Rodriguez
title Use of Busulfan to Condition Mice for Bone Marrow Transplantation
title_short Use of Busulfan to Condition Mice for Bone Marrow Transplantation
title_full Use of Busulfan to Condition Mice for Bone Marrow Transplantation
title_fullStr Use of Busulfan to Condition Mice for Bone Marrow Transplantation
title_full_unstemmed Use of Busulfan to Condition Mice for Bone Marrow Transplantation
title_sort use of busulfan to condition mice for bone marrow transplantation
publisher Elsevier
series STAR Protocols
issn 2666-1667
publishDate 2020-12-01
description Summary: Myeloablative gamma irradiation has traditionally been used to condition mice prior to bone marrow transplantation. However, irradiation induces high levels of inflammation that may alter patterns of reconstitution. In addition, gamma irradiators are being removed from many facilities for security reasons. Alternative conditioning regimens are thus needed. Here, we describe a protocol for the use of busulfan to condition mice for bone marrow transplantation and several of the variables to consider for effective implementation.For complete details on the use and execution of this protocol, please refer to Montecino-Rodriguez et al. (2019).
url http://www.sciencedirect.com/science/article/pii/S2666166720301465
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AT kennethdorshkind useofbusulfantoconditionmiceforbonemarrowtransplantation
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