The role of NF-κB signaling in the maintenance of pluripotency of human induced pluripotent stem cells.
NF-κB signaling plays an essential role in maintaining the undifferentiated state of embryonic stem (ES) cells. However, opposing roles of NF-κB have been reported in mouse and human ES cells, and the role of NF-κB in human induced pluripotent stem (iPS) cells has not yet been clarified. Here, we re...
Main Authors: | , , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2013-01-01
|
Series: | PLoS ONE |
Online Access: | http://europepmc.org/articles/PMC3577895?pdf=render |
id |
doaj-14c64e79d436496591bf9bd6022fbc1e |
---|---|
record_format |
Article |
spelling |
doaj-14c64e79d436496591bf9bd6022fbc1e2020-11-25T01:42:24ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0182e5639910.1371/journal.pone.0056399The role of NF-κB signaling in the maintenance of pluripotency of human induced pluripotent stem cells.Osamu TakaseMasahiro YoshikawaMana IdeiJunichi HirahashiToshiro FujitaTsuyoshi TakatoTakayuki IsagawaGenta NagaeHirofumi SuemoriHiroyuki AburataniKeiichi HishikawaNF-κB signaling plays an essential role in maintaining the undifferentiated state of embryonic stem (ES) cells. However, opposing roles of NF-κB have been reported in mouse and human ES cells, and the role of NF-κB in human induced pluripotent stem (iPS) cells has not yet been clarified. Here, we report the role of NF-κB signaling in maintaining the undifferentiated state of human iPS cells. Compared with differentiated cells, undifferentiated human iPS cells showed an augmentation of NF-κB activity. During differentiation induced by the removal of feeder cells and FGF2, we observed a reduction in NF-κB activity, the expression of the undifferentiation markers Oct3/4 and Nanog, and the up-regulation of the differentiated markers WT-1 and Pax-2. The specific knockdown of NF-κB signaling using p65 siRNA also reduced the expression of Oct3/4 and Nanog and up-regulated WT-1 and Pax-2 but did not change the ES-like colony formation. Our results show that the augmentation of NF-κB signaling maintains the undifferentiated state of human iPS and suggest the importance of this signaling pathway in maintenance of human iPS cells.http://europepmc.org/articles/PMC3577895?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Osamu Takase Masahiro Yoshikawa Mana Idei Junichi Hirahashi Toshiro Fujita Tsuyoshi Takato Takayuki Isagawa Genta Nagae Hirofumi Suemori Hiroyuki Aburatani Keiichi Hishikawa |
spellingShingle |
Osamu Takase Masahiro Yoshikawa Mana Idei Junichi Hirahashi Toshiro Fujita Tsuyoshi Takato Takayuki Isagawa Genta Nagae Hirofumi Suemori Hiroyuki Aburatani Keiichi Hishikawa The role of NF-κB signaling in the maintenance of pluripotency of human induced pluripotent stem cells. PLoS ONE |
author_facet |
Osamu Takase Masahiro Yoshikawa Mana Idei Junichi Hirahashi Toshiro Fujita Tsuyoshi Takato Takayuki Isagawa Genta Nagae Hirofumi Suemori Hiroyuki Aburatani Keiichi Hishikawa |
author_sort |
Osamu Takase |
title |
The role of NF-κB signaling in the maintenance of pluripotency of human induced pluripotent stem cells. |
title_short |
The role of NF-κB signaling in the maintenance of pluripotency of human induced pluripotent stem cells. |
title_full |
The role of NF-κB signaling in the maintenance of pluripotency of human induced pluripotent stem cells. |
title_fullStr |
The role of NF-κB signaling in the maintenance of pluripotency of human induced pluripotent stem cells. |
title_full_unstemmed |
The role of NF-κB signaling in the maintenance of pluripotency of human induced pluripotent stem cells. |
title_sort |
role of nf-κb signaling in the maintenance of pluripotency of human induced pluripotent stem cells. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2013-01-01 |
description |
NF-κB signaling plays an essential role in maintaining the undifferentiated state of embryonic stem (ES) cells. However, opposing roles of NF-κB have been reported in mouse and human ES cells, and the role of NF-κB in human induced pluripotent stem (iPS) cells has not yet been clarified. Here, we report the role of NF-κB signaling in maintaining the undifferentiated state of human iPS cells. Compared with differentiated cells, undifferentiated human iPS cells showed an augmentation of NF-κB activity. During differentiation induced by the removal of feeder cells and FGF2, we observed a reduction in NF-κB activity, the expression of the undifferentiation markers Oct3/4 and Nanog, and the up-regulation of the differentiated markers WT-1 and Pax-2. The specific knockdown of NF-κB signaling using p65 siRNA also reduced the expression of Oct3/4 and Nanog and up-regulated WT-1 and Pax-2 but did not change the ES-like colony formation. Our results show that the augmentation of NF-κB signaling maintains the undifferentiated state of human iPS and suggest the importance of this signaling pathway in maintenance of human iPS cells. |
url |
http://europepmc.org/articles/PMC3577895?pdf=render |
work_keys_str_mv |
AT osamutakase theroleofnfkbsignalinginthemaintenanceofpluripotencyofhumaninducedpluripotentstemcells AT masahiroyoshikawa theroleofnfkbsignalinginthemaintenanceofpluripotencyofhumaninducedpluripotentstemcells AT manaidei theroleofnfkbsignalinginthemaintenanceofpluripotencyofhumaninducedpluripotentstemcells AT junichihirahashi theroleofnfkbsignalinginthemaintenanceofpluripotencyofhumaninducedpluripotentstemcells AT toshirofujita theroleofnfkbsignalinginthemaintenanceofpluripotencyofhumaninducedpluripotentstemcells AT tsuyoshitakato theroleofnfkbsignalinginthemaintenanceofpluripotencyofhumaninducedpluripotentstemcells AT takayukiisagawa theroleofnfkbsignalinginthemaintenanceofpluripotencyofhumaninducedpluripotentstemcells AT gentanagae theroleofnfkbsignalinginthemaintenanceofpluripotencyofhumaninducedpluripotentstemcells AT hirofumisuemori theroleofnfkbsignalinginthemaintenanceofpluripotencyofhumaninducedpluripotentstemcells AT hiroyukiaburatani theroleofnfkbsignalinginthemaintenanceofpluripotencyofhumaninducedpluripotentstemcells AT keiichihishikawa theroleofnfkbsignalinginthemaintenanceofpluripotencyofhumaninducedpluripotentstemcells AT osamutakase roleofnfkbsignalinginthemaintenanceofpluripotencyofhumaninducedpluripotentstemcells AT masahiroyoshikawa roleofnfkbsignalinginthemaintenanceofpluripotencyofhumaninducedpluripotentstemcells AT manaidei roleofnfkbsignalinginthemaintenanceofpluripotencyofhumaninducedpluripotentstemcells AT junichihirahashi roleofnfkbsignalinginthemaintenanceofpluripotencyofhumaninducedpluripotentstemcells AT toshirofujita roleofnfkbsignalinginthemaintenanceofpluripotencyofhumaninducedpluripotentstemcells AT tsuyoshitakato roleofnfkbsignalinginthemaintenanceofpluripotencyofhumaninducedpluripotentstemcells AT takayukiisagawa roleofnfkbsignalinginthemaintenanceofpluripotencyofhumaninducedpluripotentstemcells AT gentanagae roleofnfkbsignalinginthemaintenanceofpluripotencyofhumaninducedpluripotentstemcells AT hirofumisuemori roleofnfkbsignalinginthemaintenanceofpluripotencyofhumaninducedpluripotentstemcells AT hiroyukiaburatani roleofnfkbsignalinginthemaintenanceofpluripotencyofhumaninducedpluripotentstemcells AT keiichihishikawa roleofnfkbsignalinginthemaintenanceofpluripotencyofhumaninducedpluripotentstemcells |
_version_ |
1725036730973683712 |