Silver Nanoparticles Functionalized by Fluorescein Isothiocyanate or Rhodamine B Isothiocyanate: Fluorescent and Plasmonic Materials
This paper presents the synthesis of silver nanoparticles (AgNPs) functionalized with fluorescent molecules, in particular with xanthene-based dyes, i.e., fluorescein isothiocyanate (FITC, λ<sub>max</sub> = 485 nm) and rhodamine B isothiocyanate (RITC, λ<sub>max</sub> = 555 n...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-03-01
|
| Series: | Applied Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-3417/11/6/2472 |
| id |
doaj-14d3f998fde647179f4cab89d692a5b6 |
|---|---|
| record_format |
Article |
| spelling |
doaj-14d3f998fde647179f4cab89d692a5b62021-03-11T00:03:41ZengMDPI AGApplied Sciences2076-34172021-03-01112472247210.3390/app11062472Silver Nanoparticles Functionalized by Fluorescein Isothiocyanate or Rhodamine B Isothiocyanate: Fluorescent and Plasmonic MaterialsIlaria Fratoddi0Chiara Battocchio1Giovanna Iucci2Daniele Catone3Antonella Cartoni4Alessandra Paladini5Patrick O’Keeffe6Silvia Nappini7Sara Cerra8Iole Venditti9Department of Chemistry, Sapienza University of Rome, P.le A. Moro, 5, 00185 Rome, ItalyDepartment of Sciences, Roma Tre University Via della Vasca Navale 79, 00146 Rome, ItalyDepartment of Sciences, Roma Tre University Via della Vasca Navale 79, 00146 Rome, ItalyIstituto di Struttura della Materia-CNR (ISM-CNR), Division of Ultrafast Processes in Materials (FLASHit), Via del Fosso del Cavaliere 100, 00133 Rome, ItalyDepartment of Chemistry, Sapienza University of Rome, P.le A. Moro, 5, 00185 Rome, ItalyIstituto di Struttura della Materia-CNR (ISM-CNR), Division of Ultrafast Processes in Materials (FLASHit), 00015 Monterotondo Scalo, ItalyIstituto di Struttura della Materia-CNR (ISM-CNR), Division of Ultrafast Processes in Materials (FLASHit), 00015 Monterotondo Scalo, ItalyIOM CNR, Laboratorio TASC, S.S. 14 Km 163.5 AREA Science Park Basovizza, 34149 Trieste, ItalyDepartment of Chemistry, Sapienza University of Rome, P.le A. Moro, 5, 00185 Rome, ItalyDepartment of Sciences, Roma Tre University Via della Vasca Navale 79, 00146 Rome, ItalyThis paper presents the synthesis of silver nanoparticles (AgNPs) functionalized with fluorescent molecules, in particular with xanthene-based dyes, i.e., fluorescein isothiocyanate (FITC, λ<sub>max</sub> = 485 nm) and rhodamine B isothiocyanate (RITC, λ<sub>max</sub> = 555 nm). An in-depth characterization of the particle–dye systems, i.e., AgNPs–RITC and AgNPs–FITC, is presented to evaluate their chemical structure and optical properties due to the interaction between their plasmonic and absorption properties. UV–Vis spectroscopy and the dynamic light scattering (DLS) measurements confirmed the nanosize of the AgNPs–RITC and AgNPs–FITC. Synchrotron radiation X-ray photoelectron spectroscopy (SR-XPS) was used to study the chemical surface functionalization by structural characterization, confirming/examining the isothiocyanate–metal interaction. For AgNPs–RITC, in which the plasmonic and fluorescence peak are not superimposed, the transient dynamics of the dye fluorescence were also studied. Transient absorption measurements showed that by exciting the AgNPs–RITC sample at a wavelength corresponding to the AgNP plasmon resonance, it was possible to preferentially excite the RITC dye molecules attached to the surface of the NPs with respect to the free dye molecules in the solution. These results demonstrate how, by combining plasmonics and fluorescence, these AgNPs can be used as promising systems in biosensing and imaging applications.https://www.mdpi.com/2076-3417/11/6/2472silver nanoparticlesrhodamine B isothiocyanatefluorescein isothiocyanatefluorescencesurface plasmon resonanceplasmonic materials |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Ilaria Fratoddi Chiara Battocchio Giovanna Iucci Daniele Catone Antonella Cartoni Alessandra Paladini Patrick O’Keeffe Silvia Nappini Sara Cerra Iole Venditti |
| spellingShingle |
Ilaria Fratoddi Chiara Battocchio Giovanna Iucci Daniele Catone Antonella Cartoni Alessandra Paladini Patrick O’Keeffe Silvia Nappini Sara Cerra Iole Venditti Silver Nanoparticles Functionalized by Fluorescein Isothiocyanate or Rhodamine B Isothiocyanate: Fluorescent and Plasmonic Materials Applied Sciences silver nanoparticles rhodamine B isothiocyanate fluorescein isothiocyanate fluorescence surface plasmon resonance plasmonic materials |
| author_facet |
Ilaria Fratoddi Chiara Battocchio Giovanna Iucci Daniele Catone Antonella Cartoni Alessandra Paladini Patrick O’Keeffe Silvia Nappini Sara Cerra Iole Venditti |
| author_sort |
Ilaria Fratoddi |
| title |
Silver Nanoparticles Functionalized by Fluorescein Isothiocyanate or Rhodamine B Isothiocyanate: Fluorescent and Plasmonic Materials |
| title_short |
Silver Nanoparticles Functionalized by Fluorescein Isothiocyanate or Rhodamine B Isothiocyanate: Fluorescent and Plasmonic Materials |
| title_full |
Silver Nanoparticles Functionalized by Fluorescein Isothiocyanate or Rhodamine B Isothiocyanate: Fluorescent and Plasmonic Materials |
| title_fullStr |
Silver Nanoparticles Functionalized by Fluorescein Isothiocyanate or Rhodamine B Isothiocyanate: Fluorescent and Plasmonic Materials |
| title_full_unstemmed |
Silver Nanoparticles Functionalized by Fluorescein Isothiocyanate or Rhodamine B Isothiocyanate: Fluorescent and Plasmonic Materials |
| title_sort |
silver nanoparticles functionalized by fluorescein isothiocyanate or rhodamine b isothiocyanate: fluorescent and plasmonic materials |
| publisher |
MDPI AG |
| series |
Applied Sciences |
| issn |
2076-3417 |
| publishDate |
2021-03-01 |
| description |
This paper presents the synthesis of silver nanoparticles (AgNPs) functionalized with fluorescent molecules, in particular with xanthene-based dyes, i.e., fluorescein isothiocyanate (FITC, λ<sub>max</sub> = 485 nm) and rhodamine B isothiocyanate (RITC, λ<sub>max</sub> = 555 nm). An in-depth characterization of the particle–dye systems, i.e., AgNPs–RITC and AgNPs–FITC, is presented to evaluate their chemical structure and optical properties due to the interaction between their plasmonic and absorption properties. UV–Vis spectroscopy and the dynamic light scattering (DLS) measurements confirmed the nanosize of the AgNPs–RITC and AgNPs–FITC. Synchrotron radiation X-ray photoelectron spectroscopy (SR-XPS) was used to study the chemical surface functionalization by structural characterization, confirming/examining the isothiocyanate–metal interaction. For AgNPs–RITC, in which the plasmonic and fluorescence peak are not superimposed, the transient dynamics of the dye fluorescence were also studied. Transient absorption measurements showed that by exciting the AgNPs–RITC sample at a wavelength corresponding to the AgNP plasmon resonance, it was possible to preferentially excite the RITC dye molecules attached to the surface of the NPs with respect to the free dye molecules in the solution. These results demonstrate how, by combining plasmonics and fluorescence, these AgNPs can be used as promising systems in biosensing and imaging applications. |
| topic |
silver nanoparticles rhodamine B isothiocyanate fluorescein isothiocyanate fluorescence surface plasmon resonance plasmonic materials |
| url |
https://www.mdpi.com/2076-3417/11/6/2472 |
| work_keys_str_mv |
AT ilariafratoddi silvernanoparticlesfunctionalizedbyfluoresceinisothiocyanateorrhodaminebisothiocyanatefluorescentandplasmonicmaterials AT chiarabattocchio silvernanoparticlesfunctionalizedbyfluoresceinisothiocyanateorrhodaminebisothiocyanatefluorescentandplasmonicmaterials AT giovannaiucci silvernanoparticlesfunctionalizedbyfluoresceinisothiocyanateorrhodaminebisothiocyanatefluorescentandplasmonicmaterials AT danielecatone silvernanoparticlesfunctionalizedbyfluoresceinisothiocyanateorrhodaminebisothiocyanatefluorescentandplasmonicmaterials AT antonellacartoni silvernanoparticlesfunctionalizedbyfluoresceinisothiocyanateorrhodaminebisothiocyanatefluorescentandplasmonicmaterials AT alessandrapaladini silvernanoparticlesfunctionalizedbyfluoresceinisothiocyanateorrhodaminebisothiocyanatefluorescentandplasmonicmaterials AT patrickokeeffe silvernanoparticlesfunctionalizedbyfluoresceinisothiocyanateorrhodaminebisothiocyanatefluorescentandplasmonicmaterials AT silvianappini silvernanoparticlesfunctionalizedbyfluoresceinisothiocyanateorrhodaminebisothiocyanatefluorescentandplasmonicmaterials AT saracerra silvernanoparticlesfunctionalizedbyfluoresceinisothiocyanateorrhodaminebisothiocyanatefluorescentandplasmonicmaterials AT iolevenditti silvernanoparticlesfunctionalizedbyfluoresceinisothiocyanateorrhodaminebisothiocyanatefluorescentandplasmonicmaterials |
| _version_ |
1724226201876168704 |