Evaluation of the Chinese Medicinal Herb, Graptopetalum paraguayense, as a Therapeutic Treatment for Liver Damage in Rat Models
The incidence of cirrhosis is rising due to the widespread occurrence of chronic hepatitis, as well as the evident lack of an established therapy for hepatic fibrosis. In the search for hepatoprotective therapeutic agents, Graptopetalum paraguayense (GP) showed greater cytotoxicity toward hepatic st...
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doaj-14deb5a4e0534219bad8ae607bc5fcca2020-11-24T23:44:08ZengHindawi LimitedEvidence-Based Complementary and Alternative Medicine1741-427X1741-42882012-01-01201210.1155/2012/256561256561Evaluation of the Chinese Medicinal Herb, Graptopetalum paraguayense, as a Therapeutic Treatment for Liver Damage in Rat ModelsLi-Jen Su0Chih-Hsueh Yang1Shiu-Feng Huang2Ya-Ling Yuo3Hui-Chu Hsieh4Tzu-Ling Tseng5Chang-Han Chen6Shih-Lan Hsu7Chi-Ying F. Huang8Institute of Systems Biology and Bioinformatics, National Central University, Jhongli City 32001, TaiwanGraduate Institute of Microbiology and Public Health, National Chung Hsing University, Taichung 40227, TaiwanDivision of Molecular and Genomic Medicine, National Health Research Institutes, Miaoli County, Zhunan 35053, TaiwanDivision of Molecular and Genomic Medicine, National Health Research Institutes, Miaoli County, Zhunan 35053, TaiwanBiomarker Technology Development Division, Biomedical Technology and Device Research Labs, Industrial Technology Research Institute, Hsinchu 31040, TaiwanBiomarker Technology Development Division, Biomedical Technology and Device Research Labs, Industrial Technology Research Institute, Hsinchu 31040, TaiwanCenter for Translational Research in Biomedical Sciences, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung 83301, TaiwanDepartment of Education and Research, Taichung Veterans General Hospital, Taichung 40705, TaiwanInstitute of Biopharmaceutical Sciences, National Yang-Ming University, Taipei 11221, TaiwanThe incidence of cirrhosis is rising due to the widespread occurrence of chronic hepatitis, as well as the evident lack of an established therapy for hepatic fibrosis. In the search for hepatoprotective therapeutic agents, Graptopetalum paraguayense (GP) showed greater cytotoxicity toward hepatic stellate cells than other tested herbal medicines. Histopathological and biochemical analyses suggest that GP treatment significantly prevented DMN-induced hepatic inflammation and fibrosis in rats. Microarray profiling indicated that expression of most of metabolism- and cell growth and/or maintenance-related genes recovered to near normal levels following GP treatment as classified by gene ontology and LSM analysis, was observed. ANOVA showed that expression of 64% of 256 liver damage-related genes recovered significantly after GP treatment. By examining rat liver samples with Q-RT-PCR, five liver damage-related genes were identified. Among them, Egr1 and Nrg1 may serve as necroinflammatory markers, and Btg2 may serve as a fibrosis marker. Oldr1 and Hmgcs1 were up- and down-regulated markers, respectively. A publicly accessible website has been established to provide access to these data Identification of 44 necroinflammation-related and 62 fibrosis-related genes provides useful insight into the molecular mechanisms underlying liver damage and provides potential targets for the rational development of therapeutic drugs such as GP.http://dx.doi.org/10.1155/2012/256561 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Li-Jen Su Chih-Hsueh Yang Shiu-Feng Huang Ya-Ling Yuo Hui-Chu Hsieh Tzu-Ling Tseng Chang-Han Chen Shih-Lan Hsu Chi-Ying F. Huang |
spellingShingle |
Li-Jen Su Chih-Hsueh Yang Shiu-Feng Huang Ya-Ling Yuo Hui-Chu Hsieh Tzu-Ling Tseng Chang-Han Chen Shih-Lan Hsu Chi-Ying F. Huang Evaluation of the Chinese Medicinal Herb, Graptopetalum paraguayense, as a Therapeutic Treatment for Liver Damage in Rat Models Evidence-Based Complementary and Alternative Medicine |
author_facet |
Li-Jen Su Chih-Hsueh Yang Shiu-Feng Huang Ya-Ling Yuo Hui-Chu Hsieh Tzu-Ling Tseng Chang-Han Chen Shih-Lan Hsu Chi-Ying F. Huang |
author_sort |
Li-Jen Su |
title |
Evaluation of the Chinese Medicinal Herb, Graptopetalum paraguayense, as a Therapeutic Treatment for Liver Damage in Rat Models |
title_short |
Evaluation of the Chinese Medicinal Herb, Graptopetalum paraguayense, as a Therapeutic Treatment for Liver Damage in Rat Models |
title_full |
Evaluation of the Chinese Medicinal Herb, Graptopetalum paraguayense, as a Therapeutic Treatment for Liver Damage in Rat Models |
title_fullStr |
Evaluation of the Chinese Medicinal Herb, Graptopetalum paraguayense, as a Therapeutic Treatment for Liver Damage in Rat Models |
title_full_unstemmed |
Evaluation of the Chinese Medicinal Herb, Graptopetalum paraguayense, as a Therapeutic Treatment for Liver Damage in Rat Models |
title_sort |
evaluation of the chinese medicinal herb, graptopetalum paraguayense, as a therapeutic treatment for liver damage in rat models |
publisher |
Hindawi Limited |
series |
Evidence-Based Complementary and Alternative Medicine |
issn |
1741-427X 1741-4288 |
publishDate |
2012-01-01 |
description |
The incidence of cirrhosis is rising due to the widespread occurrence of chronic hepatitis, as well as the evident lack of an established therapy for hepatic fibrosis. In the search for hepatoprotective therapeutic agents, Graptopetalum paraguayense (GP) showed greater cytotoxicity toward hepatic stellate cells than other tested herbal medicines. Histopathological and biochemical analyses suggest that GP treatment significantly prevented DMN-induced hepatic inflammation and fibrosis in rats. Microarray profiling indicated that expression of most of metabolism- and cell growth and/or maintenance-related genes recovered to near normal levels following GP treatment as classified by gene ontology and LSM analysis, was observed. ANOVA showed that expression of 64% of 256 liver damage-related genes recovered significantly after GP treatment. By examining rat liver samples with Q-RT-PCR, five liver damage-related genes were identified. Among them, Egr1 and Nrg1 may serve as necroinflammatory markers, and Btg2 may serve as a fibrosis marker. Oldr1 and Hmgcs1 were up- and down-regulated markers, respectively. A publicly accessible website has been established to provide access to these data Identification of 44 necroinflammation-related and 62 fibrosis-related genes provides useful insight into the molecular mechanisms underlying liver damage and provides potential targets for the rational development of therapeutic drugs such as GP. |
url |
http://dx.doi.org/10.1155/2012/256561 |
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