FRAP analysis: accounting for bleaching during image capture.

FRAP analysis: accounting for bleaching during image capture.

The analysis of Fluorescence Recovery After Photobleaching (FRAP) experiments involves mathematical modeling of the fluorescence recovery process. An important feature of FRAP experiments that tends to be ignored in the modeling is that there can be a significant loss of fluorescence due to bleachin...

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Main Authors: Jun Wu, Nandini Shekhar, Pushkar P Lele, Tanmay P Lele
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2012-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3415426?pdf=render
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spelling doaj-14e373fc5c824400a27cc9639aed08262020-11-24T22:02:02ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0178e4285410.1371/journal.pone.0042854FRAP analysis: accounting for bleaching during image capture.Jun WuNandini ShekharPushkar P LeleTanmay P LeleThe analysis of Fluorescence Recovery After Photobleaching (FRAP) experiments involves mathematical modeling of the fluorescence recovery process. An important feature of FRAP experiments that tends to be ignored in the modeling is that there can be a significant loss of fluorescence due to bleaching during image capture. In this paper, we explicitly include the effects of bleaching during image capture in the model for the recovery process, instead of correcting for the effects of bleaching using reference measurements. Using experimental examples, we demonstrate the usefulness of such an approach in FRAP analysis.http://europepmc.org/articles/PMC3415426?pdf=render
collection DOAJ
language English
format Article
sources DOAJ
author Jun Wu
Nandini Shekhar
Pushkar P Lele
Tanmay P Lele
spellingShingle Jun Wu
Nandini Shekhar
Pushkar P Lele
Tanmay P Lele
FRAP analysis: accounting for bleaching during image capture.
PLoS ONE
author_facet Jun Wu
Nandini Shekhar
Pushkar P Lele
Tanmay P Lele
author_sort Jun Wu
title FRAP analysis: accounting for bleaching during image capture.
title_short FRAP analysis: accounting for bleaching during image capture.
title_full FRAP analysis: accounting for bleaching during image capture.
title_fullStr FRAP analysis: accounting for bleaching during image capture.
title_full_unstemmed FRAP analysis: accounting for bleaching during image capture.
title_sort frap analysis: accounting for bleaching during image capture.
publisher Public Library of Science (PLoS)
series PLoS ONE
issn 1932-6203
publishDate 2012-01-01
description The analysis of Fluorescence Recovery After Photobleaching (FRAP) experiments involves mathematical modeling of the fluorescence recovery process. An important feature of FRAP experiments that tends to be ignored in the modeling is that there can be a significant loss of fluorescence due to bleaching during image capture. In this paper, we explicitly include the effects of bleaching during image capture in the model for the recovery process, instead of correcting for the effects of bleaching using reference measurements. Using experimental examples, we demonstrate the usefulness of such an approach in FRAP analysis.
url http://europepmc.org/articles/PMC3415426?pdf=render
work_keys_str_mv AT junwu frapanalysisaccountingforbleachingduringimagecapture
AT nandinishekhar frapanalysisaccountingforbleachingduringimagecapture
AT pushkarplele frapanalysisaccountingforbleachingduringimagecapture
AT tanmayplele frapanalysisaccountingforbleachingduringimagecapture
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