Isotope-Labeled RNA Building Blocks for NMR Structure and Dynamics Studies

RNA structural research lags behind that of proteins, preventing a robust understanding of RNA functions. NMR spectroscopy is an apt technique for probing the structures and dynamics of RNA molecules in solution at atomic resolution. Still, RNA analysis by NMR suffers from spectral overlap and line...

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Main Authors:	Lukasz T. Olenginski, Kehinde M. Taiwo, Regan M. LeBlanc, Theodore K. Dayie
Format:	Article
Language:	English
Published:	MDPI AG 2021-09-01
Series:	Molecules
Subjects:	stable isotope enzymes nucleotides NMR spectroscopy RNA structure
Online Access:	https://www.mdpi.com/1420-3049/26/18/5581

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record_format	Article
spelling	doaj-14e4d91960de4b8eb84cace903777a302021-09-26T00:46:29ZengMDPI AGMolecules1420-30492021-09-01265581558110.3390/molecules26185581Isotope-Labeled RNA Building Blocks for NMR Structure and Dynamics StudiesLukasz T. Olenginski0Kehinde M. Taiwo1Regan M. LeBlanc2Theodore K. Dayie3Center for Biomolecular Structure and Organization, Department of Chemistry and Biochemistry, University of Maryland, College Park, MD 20742, USACenter for Biomolecular Structure and Organization, Department of Chemistry and Biochemistry, University of Maryland, College Park, MD 20742, USACenter for Biomolecular Structure and Organization, Department of Chemistry and Biochemistry, University of Maryland, College Park, MD 20742, USACenter for Biomolecular Structure and Organization, Department of Chemistry and Biochemistry, University of Maryland, College Park, MD 20742, USARNA structural research lags behind that of proteins, preventing a robust understanding of RNA functions. NMR spectroscopy is an apt technique for probing the structures and dynamics of RNA molecules in solution at atomic resolution. Still, RNA analysis by NMR suffers from spectral overlap and line broadening, both of which worsen for larger RNAs. Incorporation of stable isotope labels into RNA has provided several solutions to these challenges. In this review, we summarize the benefits and limitations of various methods used to obtain isotope-labeled RNA building blocks and how they are used to prepare isotope-labeled RNA for NMR structure and dynamics studies.https://www.mdpi.com/1420-3049/26/18/5581stable isotopeenzymesnucleotidesNMR spectroscopyRNAstructure
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Lukasz T. Olenginski Kehinde M. Taiwo Regan M. LeBlanc Theodore K. Dayie
spellingShingle	Lukasz T. Olenginski Kehinde M. Taiwo Regan M. LeBlanc Theodore K. Dayie Isotope-Labeled RNA Building Blocks for NMR Structure and Dynamics Studies Molecules stable isotope enzymes nucleotides NMR spectroscopy RNA structure
author_facet	Lukasz T. Olenginski Kehinde M. Taiwo Regan M. LeBlanc Theodore K. Dayie
author_sort	Lukasz T. Olenginski
title	Isotope-Labeled RNA Building Blocks for NMR Structure and Dynamics Studies
title_short	Isotope-Labeled RNA Building Blocks for NMR Structure and Dynamics Studies
title_full	Isotope-Labeled RNA Building Blocks for NMR Structure and Dynamics Studies
title_fullStr	Isotope-Labeled RNA Building Blocks for NMR Structure and Dynamics Studies
title_full_unstemmed	Isotope-Labeled RNA Building Blocks for NMR Structure and Dynamics Studies
title_sort	isotope-labeled rna building blocks for nmr structure and dynamics studies
publisher	MDPI AG
series	Molecules
issn	1420-3049
publishDate	2021-09-01
description	RNA structural research lags behind that of proteins, preventing a robust understanding of RNA functions. NMR spectroscopy is an apt technique for probing the structures and dynamics of RNA molecules in solution at atomic resolution. Still, RNA analysis by NMR suffers from spectral overlap and line broadening, both of which worsen for larger RNAs. Incorporation of stable isotope labels into RNA has provided several solutions to these challenges. In this review, we summarize the benefits and limitations of various methods used to obtain isotope-labeled RNA building blocks and how they are used to prepare isotope-labeled RNA for NMR structure and dynamics studies.
topic	stable isotope enzymes nucleotides NMR spectroscopy RNA structure
url	https://www.mdpi.com/1420-3049/26/18/5581
work_keys_str_mv	AT lukasztolenginski isotopelabeledrnabuildingblocksfornmrstructureanddynamicsstudies AT kehindemtaiwo isotopelabeledrnabuildingblocksfornmrstructureanddynamicsstudies AT reganmleblanc isotopelabeledrnabuildingblocksfornmrstructureanddynamicsstudies AT theodorekdayie isotopelabeledrnabuildingblocksfornmrstructureanddynamicsstudies
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Isotope-Labeled RNA Building Blocks for NMR Structure and Dynamics Studies

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