Intradermal Alcian-Blue Injection Method to Trace Acupuncture Meridians
Objective : In this article, we report on the intradermal Alcian blue staining method for tracing the meridians of acupuncture. Methods : 1% Alcian blue solution was injected into acupoints by using a 0.5mL insulin syringe with a 31-gauge needle, then the skin was incised and was observed under a s...
Main Authors: | , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Korean Pharmacopuncture Institute
2008-06-01
|
Series: | Journal of Pharmacopuncture |
Subjects: | |
Online Access: | http://dx.doi.org/10.3831/KPI.2008.11.2.005 |
id |
doaj-14e9e1eee7bb4def96d98f51b535588f |
---|---|
record_format |
Article |
spelling |
doaj-14e9e1eee7bb4def96d98f51b535588f2020-11-25T02:32:11ZengKorean Pharmacopuncture InstituteJournal of Pharmacopuncture2093-69662234-68562008-06-0111251210.3831/KPI.2008.11.2.005Intradermal Alcian-Blue Injection Method to Trace Acupuncture MeridiansBaeckkyoung Sung0Min-Su Kim21Vyacheslav Ogay2Dae-In Kang3Kwang-Sup Soh4Biomedical Physics Laboratory, Department of Physics and Astronomy, Seoul National University, Seoul 151-747, KoreaDepartment of Veterinary Surgery, College of Veterinary Medicine, Chonbuk National University, Jeonju 664-14, KoreaBiomedical Physics Laboratory, Department of Physics and Astronomy, Seoul National University, Seoul 151-747, KoreaKorean Pharmacopuncture InstituteBiomedical Physics Laboratory, Department of Physics and Astronomy, Seoul National University, Seoul 151-747, KoreaObjective : In this article, we report on the intradermal Alcian blue staining method for tracing the meridians of acupuncture. Methods : 1% Alcian blue solution was injected into acupoints by using a 0.5mL insulin syringe with a 31-gauge needle, then the skin was incised and was observed under a stereoscopic microscope. The specimens were examined by using immunohistochemical methods and were observed under a confocal laser scanning microscope. Results : A threadlike structure, which was visualized with Alcian blue, existed in dermis layer and proceeded to hypodermis. In this structure, characteristic alignments of rod- shaped nuclei and 1-2μm sized DNA granules were observed. Furthermore, abundant blood capillary plexuses, peripheral nerve endings, and a corpusclelike structure(about 300μm in diameter) were visualized in the skin tissues of acupoints. Conclusion : It was concluded that the specific threadlike and corpuscle-like structures corresponded to superficial Bonghan duct and corpuscle, respectively.http://dx.doi.org/10.3831/KPI.2008.11.2.005AcupointIntradermal injectionAlcian blueTracingSuperficial Bonghan duct and corpuscleImmunohistochemistryDrug delivery |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Baeckkyoung Sung Min-Su Kim2 Vyacheslav Ogay Dae-In Kang Kwang-Sup Soh |
spellingShingle |
Baeckkyoung Sung Min-Su Kim2 Vyacheslav Ogay Dae-In Kang Kwang-Sup Soh Intradermal Alcian-Blue Injection Method to Trace Acupuncture Meridians Journal of Pharmacopuncture Acupoint Intradermal injection Alcian blue Tracing Superficial Bonghan duct and corpuscle Immunohistochemistry Drug delivery |
author_facet |
Baeckkyoung Sung Min-Su Kim2 Vyacheslav Ogay Dae-In Kang Kwang-Sup Soh |
author_sort |
Baeckkyoung Sung |
title |
Intradermal Alcian-Blue Injection Method to Trace Acupuncture Meridians |
title_short |
Intradermal Alcian-Blue Injection Method to Trace Acupuncture Meridians |
title_full |
Intradermal Alcian-Blue Injection Method to Trace Acupuncture Meridians |
title_fullStr |
Intradermal Alcian-Blue Injection Method to Trace Acupuncture Meridians |
title_full_unstemmed |
Intradermal Alcian-Blue Injection Method to Trace Acupuncture Meridians |
title_sort |
intradermal alcian-blue injection method to trace acupuncture meridians |
publisher |
Korean Pharmacopuncture Institute |
series |
Journal of Pharmacopuncture |
issn |
2093-6966 2234-6856 |
publishDate |
2008-06-01 |
description |
Objective : In this article, we report on the intradermal Alcian blue staining method for tracing the meridians of acupuncture.
Methods : 1% Alcian blue solution was injected into acupoints by using a 0.5mL insulin syringe with a 31-gauge needle, then the skin was incised and was observed under a stereoscopic microscope. The specimens were examined by using immunohistochemical methods and were observed under a confocal laser scanning microscope.
Results : A threadlike structure, which was visualized with Alcian blue, existed in dermis layer and proceeded to hypodermis. In this structure, characteristic alignments of rod- shaped nuclei and 1-2μm sized DNA granules were observed. Furthermore, abundant blood capillary plexuses, peripheral nerve endings, and a corpusclelike structure(about 300μm in diameter) were visualized in the skin tissues of acupoints.
Conclusion : It was concluded that the specific threadlike and corpuscle-like structures corresponded to superficial Bonghan
duct and corpuscle, respectively. |
topic |
Acupoint Intradermal injection Alcian blue Tracing Superficial Bonghan duct and corpuscle Immunohistochemistry Drug delivery |
url |
http://dx.doi.org/10.3831/KPI.2008.11.2.005 |
work_keys_str_mv |
AT baeckkyoungsung intradermalalcianblueinjectionmethodtotraceacupuncturemeridians AT minsukim2 intradermalalcianblueinjectionmethodtotraceacupuncturemeridians AT vyacheslavogay intradermalalcianblueinjectionmethodtotraceacupuncturemeridians AT daeinkang intradermalalcianblueinjectionmethodtotraceacupuncturemeridians AT kwangsupsoh intradermalalcianblueinjectionmethodtotraceacupuncturemeridians |
_version_ |
1724820925502717952 |