The dynamic transmission of positional information in stau- mutants during Drosophila embryogenesis
It has been suggested that Staufen (Stau) is key in controlling the variability of the posterior boundary of the Hb anterior domain (xHb). However, the mechanism that underlies this control is elusive. Here, we quantified the dynamic 3D expression of segmentation genes in Drosophila embryos. With im...
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doaj-14fbd9cb952649119fc12fbb018c86632021-05-05T21:11:13ZengeLife Sciences Publications LtdeLife2050-084X2020-06-01910.7554/eLife.54276The dynamic transmission of positional information in stau- mutants during Drosophila embryogenesisZhe Yang0Hongcun Zhu1Kakit Kong2Xiaoxuan Wu3Jiayi Chen4Peiyao Li5Jialong Jiang6Jinchao Zhao7Bofei Cui8Feng Liu9https://orcid.org/0000-0001-9724-6127State Key Laboratory of Nuclear Physics and Technology & Center for Quantitative Biology, Peking University, Beijing, China; China National Center for Biotechnology Development, Beijing, ChinaState Key Laboratory of Nuclear Physics and Technology & Center for Quantitative Biology, Peking University, Beijing, ChinaState Key Laboratory of Nuclear Physics and Technology & Center for Quantitative Biology, Peking University, Beijing, ChinaState Key Laboratory of Nuclear Physics and Technology & Center for Quantitative Biology, Peking University, Beijing, ChinaState Key Laboratory of Nuclear Physics and Technology & Center for Quantitative Biology, Peking University, Beijing, ChinaState Key Laboratory of Nuclear Physics and Technology & Center for Quantitative Biology, Peking University, Beijing, ChinaState Key Laboratory of Nuclear Physics and Technology & Center for Quantitative Biology, Peking University, Beijing, ChinaState Key Laboratory of Nuclear Physics and Technology & Center for Quantitative Biology, Peking University, Beijing, ChinaState Key Laboratory of Nuclear Physics and Technology & Center for Quantitative Biology, Peking University, Beijing, ChinaState Key Laboratory of Nuclear Physics and Technology & Center for Quantitative Biology, Peking University, Beijing, ChinaIt has been suggested that Staufen (Stau) is key in controlling the variability of the posterior boundary of the Hb anterior domain (xHb). However, the mechanism that underlies this control is elusive. Here, we quantified the dynamic 3D expression of segmentation genes in Drosophila embryos. With improved control of measurement errors, we show that the xHb of stau– mutants reproducibly moves posteriorly by 10% of the embryo length (EL) to the wild type (WT) position in the nuclear cycle (nc) 14, and that its variability over short time windows is comparable to that of the WT. Moreover, for stau– mutants, the upstream Bicoid (Bcd) gradients show equivalent relative intensity noise to that of the WT in nc12–nc14, and the downstream Even-skipped (Eve) and cephalic furrow (CF) show the same positional errors as these factors in WT. Our results indicate that threshold-dependent activation and self-organized filtering are not mutually exclusive and could both be implemented in early Drosophila embryogenesis.https://elifesciences.org/articles/54276threshold dependent modelnoise filteringdevelopmental patterning |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Zhe Yang Hongcun Zhu Kakit Kong Xiaoxuan Wu Jiayi Chen Peiyao Li Jialong Jiang Jinchao Zhao Bofei Cui Feng Liu |
spellingShingle |
Zhe Yang Hongcun Zhu Kakit Kong Xiaoxuan Wu Jiayi Chen Peiyao Li Jialong Jiang Jinchao Zhao Bofei Cui Feng Liu The dynamic transmission of positional information in stau- mutants during Drosophila embryogenesis eLife threshold dependent model noise filtering developmental patterning |
author_facet |
Zhe Yang Hongcun Zhu Kakit Kong Xiaoxuan Wu Jiayi Chen Peiyao Li Jialong Jiang Jinchao Zhao Bofei Cui Feng Liu |
author_sort |
Zhe Yang |
title |
The dynamic transmission of positional information in stau- mutants during Drosophila embryogenesis |
title_short |
The dynamic transmission of positional information in stau- mutants during Drosophila embryogenesis |
title_full |
The dynamic transmission of positional information in stau- mutants during Drosophila embryogenesis |
title_fullStr |
The dynamic transmission of positional information in stau- mutants during Drosophila embryogenesis |
title_full_unstemmed |
The dynamic transmission of positional information in stau- mutants during Drosophila embryogenesis |
title_sort |
dynamic transmission of positional information in stau- mutants during drosophila embryogenesis |
publisher |
eLife Sciences Publications Ltd |
series |
eLife |
issn |
2050-084X |
publishDate |
2020-06-01 |
description |
It has been suggested that Staufen (Stau) is key in controlling the variability of the posterior boundary of the Hb anterior domain (xHb). However, the mechanism that underlies this control is elusive. Here, we quantified the dynamic 3D expression of segmentation genes in Drosophila embryos. With improved control of measurement errors, we show that the xHb of stau– mutants reproducibly moves posteriorly by 10% of the embryo length (EL) to the wild type (WT) position in the nuclear cycle (nc) 14, and that its variability over short time windows is comparable to that of the WT. Moreover, for stau– mutants, the upstream Bicoid (Bcd) gradients show equivalent relative intensity noise to that of the WT in nc12–nc14, and the downstream Even-skipped (Eve) and cephalic furrow (CF) show the same positional errors as these factors in WT. Our results indicate that threshold-dependent activation and self-organized filtering are not mutually exclusive and could both be implemented in early Drosophila embryogenesis. |
topic |
threshold dependent model noise filtering developmental patterning |
url |
https://elifesciences.org/articles/54276 |
work_keys_str_mv |
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