LncRNA CDKN2B-AS1 Promotes Cell Viability, Migration, and Invasion of Hepatocellular Carcinoma via Sponging miR-424-5p
Xinying Shen, Yong Li, Fan He, Jian Kong Department of Interventional Radiology, Shenzhen People’s Hospital, Shenzhen, Guangdong, People’s Republic of ChinaCorrespondence: Xinying ShenDepartment of Interventional Radiology, Shenzhen People’s Hospital, Luohu District, Sh...
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doaj-151a56439b5141f7b1df53deef876ca32020-11-25T01:58:28ZengDove Medical PressCancer Management and Research1179-13222020-08-01Volume 126807681955924LncRNA CDKN2B-AS1 Promotes Cell Viability, Migration, and Invasion of Hepatocellular Carcinoma via Sponging miR-424-5pShen XLi YHe FKong JXinying Shen, Yong Li, Fan He, Jian Kong Department of Interventional Radiology, Shenzhen People’s Hospital, Shenzhen, Guangdong, People’s Republic of ChinaCorrespondence: Xinying ShenDepartment of Interventional Radiology, Shenzhen People’s Hospital, Luohu District, Shenzhen, Guangdong 518020, People’s Republic of ChinaTel +86 755-22948106Email xiny_shenxying@163.comObjective: Hepatocellular carcinoma (HCC) results in high mortality and metastasis. In this study, the effects of long non-coding RNA (lncRNA) CDKN2B-AS1 on the progression of HCC were investigated.Materials and Methods: LncRNA CDKN2B-AS1 expression of HCC cancer and adjacent tissues, and HCC cells were detected. Subsequently, CDKN2B-AS1 was overexpressed and silenced in HCC cells to observe the effects of CDKN2B-AS1 on the cell viability, migration, invasion, and epithelial–mesenchymal transition (EMT) of HCC cells by performing cell counting kit-8 (CCK-8), wound-healing, Transwell, and Western blot. The target gene of CDKN2B-AS1 was predicted and verified to be miR-424-5p, whose expression in HCC cells with up- or down-regulation of CDKN2B-AS1 expression was determined. Moreover, the effects of miR-424-5p on cell viability, migration, and invasion and EMT of HCC cells were investigated with miR-424-5p up-regulation or down-regulation, together with overexpression or silencing of CDKN2B-AS1.Results: CDKN2B-AS1 expression was increased in HCC tissues and cells. Silencing of CDKN2B-AS1 suppressed cell viability, migration, invasion, and EMT, while overexpression of CDKN2B-AS1 produced the opposite results. Furthermore, CDKN2B-AS1 was predicted and verified to target miR-424-5p and was confirmed to negatively modulate miR-424-5p expression. Moreover, overexpression of miR-424-5p partially suppressed the previously high cell viability, migration, and invasion, and activated EMT resulted from up-regulation of CDKN2B-AS1, while silencing of miR-424-5p elevated the cellular processes inhibited by silencing the expression of CDKN2B-AS1.Conclusion: The present study revealed that high-expressed CDKN2B-AS1 may associate with the progression of HCC by affecting the cell viability, migration, invasion, and EMT of HCC cells by negatively regulating miR-424-5p.Keywords: lncRNA CDKN2B-AS1, miR-424-5p, migration, invasion, hepatocellular carcinomahttps://www.dovepress.com/lncrna-cdkn2b-as1-promotes-cell-viability-migration-and-invasion-of-he-peer-reviewed-article-CMARlncrna cdkn2b-as1mir-424-5pmigrationinvasionhepatocellular carcinoma |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Shen X Li Y He F Kong J |
spellingShingle |
Shen X Li Y He F Kong J LncRNA CDKN2B-AS1 Promotes Cell Viability, Migration, and Invasion of Hepatocellular Carcinoma via Sponging miR-424-5p Cancer Management and Research lncrna cdkn2b-as1 mir-424-5p migration invasion hepatocellular carcinoma |
author_facet |
Shen X Li Y He F Kong J |
author_sort |
Shen X |
title |
LncRNA CDKN2B-AS1 Promotes Cell Viability, Migration, and Invasion of Hepatocellular Carcinoma via Sponging miR-424-5p |
title_short |
LncRNA CDKN2B-AS1 Promotes Cell Viability, Migration, and Invasion of Hepatocellular Carcinoma via Sponging miR-424-5p |
title_full |
LncRNA CDKN2B-AS1 Promotes Cell Viability, Migration, and Invasion of Hepatocellular Carcinoma via Sponging miR-424-5p |
title_fullStr |
LncRNA CDKN2B-AS1 Promotes Cell Viability, Migration, and Invasion of Hepatocellular Carcinoma via Sponging miR-424-5p |
title_full_unstemmed |
LncRNA CDKN2B-AS1 Promotes Cell Viability, Migration, and Invasion of Hepatocellular Carcinoma via Sponging miR-424-5p |
title_sort |
lncrna cdkn2b-as1 promotes cell viability, migration, and invasion of hepatocellular carcinoma via sponging mir-424-5p |
publisher |
Dove Medical Press |
series |
Cancer Management and Research |
issn |
1179-1322 |
publishDate |
2020-08-01 |
description |
Xinying Shen, Yong Li, Fan He, Jian Kong Department of Interventional Radiology, Shenzhen People’s Hospital, Shenzhen, Guangdong, People’s Republic of ChinaCorrespondence: Xinying ShenDepartment of Interventional Radiology, Shenzhen People’s Hospital, Luohu District, Shenzhen, Guangdong 518020, People’s Republic of ChinaTel +86 755-22948106Email xiny_shenxying@163.comObjective: Hepatocellular carcinoma (HCC) results in high mortality and metastasis. In this study, the effects of long non-coding RNA (lncRNA) CDKN2B-AS1 on the progression of HCC were investigated.Materials and Methods: LncRNA CDKN2B-AS1 expression of HCC cancer and adjacent tissues, and HCC cells were detected. Subsequently, CDKN2B-AS1 was overexpressed and silenced in HCC cells to observe the effects of CDKN2B-AS1 on the cell viability, migration, invasion, and epithelial–mesenchymal transition (EMT) of HCC cells by performing cell counting kit-8 (CCK-8), wound-healing, Transwell, and Western blot. The target gene of CDKN2B-AS1 was predicted and verified to be miR-424-5p, whose expression in HCC cells with up- or down-regulation of CDKN2B-AS1 expression was determined. Moreover, the effects of miR-424-5p on cell viability, migration, and invasion and EMT of HCC cells were investigated with miR-424-5p up-regulation or down-regulation, together with overexpression or silencing of CDKN2B-AS1.Results: CDKN2B-AS1 expression was increased in HCC tissues and cells. Silencing of CDKN2B-AS1 suppressed cell viability, migration, invasion, and EMT, while overexpression of CDKN2B-AS1 produced the opposite results. Furthermore, CDKN2B-AS1 was predicted and verified to target miR-424-5p and was confirmed to negatively modulate miR-424-5p expression. Moreover, overexpression of miR-424-5p partially suppressed the previously high cell viability, migration, and invasion, and activated EMT resulted from up-regulation of CDKN2B-AS1, while silencing of miR-424-5p elevated the cellular processes inhibited by silencing the expression of CDKN2B-AS1.Conclusion: The present study revealed that high-expressed CDKN2B-AS1 may associate with the progression of HCC by affecting the cell viability, migration, invasion, and EMT of HCC cells by negatively regulating miR-424-5p.Keywords: lncRNA CDKN2B-AS1, miR-424-5p, migration, invasion, hepatocellular carcinoma |
topic |
lncrna cdkn2b-as1 mir-424-5p migration invasion hepatocellular carcinoma |
url |
https://www.dovepress.com/lncrna-cdkn2b-as1-promotes-cell-viability-migration-and-invasion-of-he-peer-reviewed-article-CMAR |
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