Detection of antibacterial activities of Miswak, Kalonji and Aloe vera against oral pathogens & anti-proliferative activity against cancer cell line
Abstract Background Emerging drug resistance and hindrance of treatment is provoking scientists to search new, less expensive medicinally active compounds. Dental diseases caused by oral pathogens are very frequent chronic infections around the world. The medical potentials of a lot of Pakistani loc...
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doaj-1526bbdfd099464e89352085d3c2f5bd2020-11-25T03:46:33ZengBMCBMC Complementary and Alternative Medicine1472-68822017-05-0117111010.1186/s12906-017-1778-0Detection of antibacterial activities of Miswak, Kalonji and Aloe vera against oral pathogens & anti-proliferative activity against cancer cell lineSameen Amjed0Kashaf Junaid1Junaid Jafar2Tuaha Amjad3Waqas Maqsood4Nadia Mukhtar5Kanza Tariq6Musarrat Sharif7Sana Javaid Awan8Farheen Ansari9Institute of Molecular Biology and Biotechnology (IMBB), The University of LahoreDepartment of University Institute of Medical Laboratory Technology, The University of LahoreDepartment of University Institute of Medical Laboratory Technology, The University of LahoreFatima Memorial College of Medicine and Dentistry LahoreDepartment of University Institute of Medical Laboratory Technology, The University of LahoreDepartment of University Institute of Medical Laboratory Technology, The University of LahoreInstitute of Molecular Biology and Biotechnology (IMBB), The University of LahoreInstitute of Molecular Biology and Biotechnology (IMBB), The University of LahoreInstitute of Molecular Biology and Biotechnology (IMBB), The University of LahoreInstitute of Molecular Biology and Biotechnology (IMBB), The University of LahoreAbstract Background Emerging drug resistance and hindrance of treatment is provoking scientists to search new, less expensive medicinally active compounds. Dental diseases caused by oral pathogens are very frequent chronic infections around the world. The medical potentials of a lot of Pakistani local herbs and herbal combinations is relatively unknown, hence attempted to explore. A study was designed to investigate potential role of local medicinal herbs for example Miswak, Kalonji & Aloe vera as antimicrobial, antioxidant and anti-proliferative agents against oral pathogens and cancer cell line. Methods Medicinal extracts were prepared in solvents of different polarities. Their antimicrobial activity was determined alone and in combination against oral pathogens. Antioxidant activity was evaluated through Catalase and Superoxide dismutase assay and anti-proliferative activity was evaluated through 3-(4, 5-Dimethylthiazol-2-Yl)-2,5-Diphenyltetrazolium Bromide) assay. Results Plant extracts alone and in combinations were found significantly effective as antimicrobial agent against standard ATCC strains of C. albicans and S. aureus (P ˂0.001). Especially Miwak extract was found highly significant against fungus. Extracts of Kalonji were found significant in inhibiting growth of HeLa cell lines. Miswak and Kalonji showed significant levels of antioxidant activity. Conclusion Medicinal herbs Miswak and Kalonji have potential to be used for therapeutic purposes. Results suggested that herbal medicinal composition can be prepared using these extracts after applying scientific standardization methods.http://link.springer.com/article/10.1186/s12906-017-1778-0Herbal medicineAntimicrobialAnti cancerAntioxidant |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sameen Amjed Kashaf Junaid Junaid Jafar Tuaha Amjad Waqas Maqsood Nadia Mukhtar Kanza Tariq Musarrat Sharif Sana Javaid Awan Farheen Ansari |
spellingShingle |
Sameen Amjed Kashaf Junaid Junaid Jafar Tuaha Amjad Waqas Maqsood Nadia Mukhtar Kanza Tariq Musarrat Sharif Sana Javaid Awan Farheen Ansari Detection of antibacterial activities of Miswak, Kalonji and Aloe vera against oral pathogens & anti-proliferative activity against cancer cell line BMC Complementary and Alternative Medicine Herbal medicine Antimicrobial Anti cancer Antioxidant |
author_facet |
Sameen Amjed Kashaf Junaid Junaid Jafar Tuaha Amjad Waqas Maqsood Nadia Mukhtar Kanza Tariq Musarrat Sharif Sana Javaid Awan Farheen Ansari |
author_sort |
Sameen Amjed |
title |
Detection of antibacterial activities of Miswak, Kalonji and Aloe vera against oral pathogens & anti-proliferative activity against cancer cell line |
title_short |
Detection of antibacterial activities of Miswak, Kalonji and Aloe vera against oral pathogens & anti-proliferative activity against cancer cell line |
title_full |
Detection of antibacterial activities of Miswak, Kalonji and Aloe vera against oral pathogens & anti-proliferative activity against cancer cell line |
title_fullStr |
Detection of antibacterial activities of Miswak, Kalonji and Aloe vera against oral pathogens & anti-proliferative activity against cancer cell line |
title_full_unstemmed |
Detection of antibacterial activities of Miswak, Kalonji and Aloe vera against oral pathogens & anti-proliferative activity against cancer cell line |
title_sort |
detection of antibacterial activities of miswak, kalonji and aloe vera against oral pathogens & anti-proliferative activity against cancer cell line |
publisher |
BMC |
series |
BMC Complementary and Alternative Medicine |
issn |
1472-6882 |
publishDate |
2017-05-01 |
description |
Abstract Background Emerging drug resistance and hindrance of treatment is provoking scientists to search new, less expensive medicinally active compounds. Dental diseases caused by oral pathogens are very frequent chronic infections around the world. The medical potentials of a lot of Pakistani local herbs and herbal combinations is relatively unknown, hence attempted to explore. A study was designed to investigate potential role of local medicinal herbs for example Miswak, Kalonji & Aloe vera as antimicrobial, antioxidant and anti-proliferative agents against oral pathogens and cancer cell line. Methods Medicinal extracts were prepared in solvents of different polarities. Their antimicrobial activity was determined alone and in combination against oral pathogens. Antioxidant activity was evaluated through Catalase and Superoxide dismutase assay and anti-proliferative activity was evaluated through 3-(4, 5-Dimethylthiazol-2-Yl)-2,5-Diphenyltetrazolium Bromide) assay. Results Plant extracts alone and in combinations were found significantly effective as antimicrobial agent against standard ATCC strains of C. albicans and S. aureus (P ˂0.001). Especially Miwak extract was found highly significant against fungus. Extracts of Kalonji were found significant in inhibiting growth of HeLa cell lines. Miswak and Kalonji showed significant levels of antioxidant activity. Conclusion Medicinal herbs Miswak and Kalonji have potential to be used for therapeutic purposes. Results suggested that herbal medicinal composition can be prepared using these extracts after applying scientific standardization methods. |
topic |
Herbal medicine Antimicrobial Anti cancer Antioxidant |
url |
http://link.springer.com/article/10.1186/s12906-017-1778-0 |
work_keys_str_mv |
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