Liver-specific γ-glutamyl carboxylase-deficient mice display bleeding diathesis and short life span.
Vitamin K is a fat-soluble vitamin that plays important roles in blood coagulation and bone metabolism. One of its functions is as a co-factor for γ-glutamyl carboxylase (Ggcx). Conventional knockout of Ggcx causes death shortly after birth in homozygous mice. We created Ggcx-floxed mice by insertin...
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2014-01-01
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doaj-152e93498b224cff9a184058adbf5bdb2020-11-24T21:54:19ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0192e8864310.1371/journal.pone.0088643Liver-specific γ-glutamyl carboxylase-deficient mice display bleeding diathesis and short life span.Kotaro AzumaTohru TsukuiKazuhiro IkedaSachiko ShibaKimie NakagawaToshio OkanoTomohiko UranoKuniko Horie-InoueYasuyoshi OuchiMasahito IkawaSatoshi InoueVitamin K is a fat-soluble vitamin that plays important roles in blood coagulation and bone metabolism. One of its functions is as a co-factor for γ-glutamyl carboxylase (Ggcx). Conventional knockout of Ggcx causes death shortly after birth in homozygous mice. We created Ggcx-floxed mice by inserting loxP sequences at the sites flanking exon 6 of Ggcx. By mating these mice with albumin-Cre mice, we generated Ggcx-deficient mice specifically in hepatocytes (Ggcx(Δliver/Δliver) mice). In contrast to conventional Ggcx knockout mice, Ggcx(Δliver/Δliver) mice had very low activity of Ggcx in the liver and survived several weeks after birth. Furthermore, compared with heterozygous mice (Ggcx(+/Δliver) ), Ggcx(Δliver/Δliver) mice had shorter life spans. Ggcx(Δliver/Δliver) mice displayed bleeding diathesis, which was accompanied by decreased activity of coagulation factors II and IX. Ggcx-floxed mice can prove useful in examining Ggcx functions in vivo.http://europepmc.org/articles/PMC3919827?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Kotaro Azuma Tohru Tsukui Kazuhiro Ikeda Sachiko Shiba Kimie Nakagawa Toshio Okano Tomohiko Urano Kuniko Horie-Inoue Yasuyoshi Ouchi Masahito Ikawa Satoshi Inoue |
spellingShingle |
Kotaro Azuma Tohru Tsukui Kazuhiro Ikeda Sachiko Shiba Kimie Nakagawa Toshio Okano Tomohiko Urano Kuniko Horie-Inoue Yasuyoshi Ouchi Masahito Ikawa Satoshi Inoue Liver-specific γ-glutamyl carboxylase-deficient mice display bleeding diathesis and short life span. PLoS ONE |
author_facet |
Kotaro Azuma Tohru Tsukui Kazuhiro Ikeda Sachiko Shiba Kimie Nakagawa Toshio Okano Tomohiko Urano Kuniko Horie-Inoue Yasuyoshi Ouchi Masahito Ikawa Satoshi Inoue |
author_sort |
Kotaro Azuma |
title |
Liver-specific γ-glutamyl carboxylase-deficient mice display bleeding diathesis and short life span. |
title_short |
Liver-specific γ-glutamyl carboxylase-deficient mice display bleeding diathesis and short life span. |
title_full |
Liver-specific γ-glutamyl carboxylase-deficient mice display bleeding diathesis and short life span. |
title_fullStr |
Liver-specific γ-glutamyl carboxylase-deficient mice display bleeding diathesis and short life span. |
title_full_unstemmed |
Liver-specific γ-glutamyl carboxylase-deficient mice display bleeding diathesis and short life span. |
title_sort |
liver-specific γ-glutamyl carboxylase-deficient mice display bleeding diathesis and short life span. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2014-01-01 |
description |
Vitamin K is a fat-soluble vitamin that plays important roles in blood coagulation and bone metabolism. One of its functions is as a co-factor for γ-glutamyl carboxylase (Ggcx). Conventional knockout of Ggcx causes death shortly after birth in homozygous mice. We created Ggcx-floxed mice by inserting loxP sequences at the sites flanking exon 6 of Ggcx. By mating these mice with albumin-Cre mice, we generated Ggcx-deficient mice specifically in hepatocytes (Ggcx(Δliver/Δliver) mice). In contrast to conventional Ggcx knockout mice, Ggcx(Δliver/Δliver) mice had very low activity of Ggcx in the liver and survived several weeks after birth. Furthermore, compared with heterozygous mice (Ggcx(+/Δliver) ), Ggcx(Δliver/Δliver) mice had shorter life spans. Ggcx(Δliver/Δliver) mice displayed bleeding diathesis, which was accompanied by decreased activity of coagulation factors II and IX. Ggcx-floxed mice can prove useful in examining Ggcx functions in vivo. |
url |
http://europepmc.org/articles/PMC3919827?pdf=render |
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