Multiplex polymerase chain reaction for pathogen detection in donor/recipient corneal transplant tissue and donor storage solution
Abstract Corneal transplantation is a safe, reliable method of restoring visual acuity in patients with corneal disorders. Although it has a very high success rate, rejection can still occur, especially if the site is infected. Therefore, seeking to find better ways to manage infection risk, this st...
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doaj-153040f092254916a6d0902b901bd8212020-12-08T00:06:53ZengNature Publishing GroupScientific Reports2045-23222017-07-01711710.1038/s41598-017-06344-3Multiplex polymerase chain reaction for pathogen detection in donor/recipient corneal transplant tissue and donor storage solutionTakehiro Hariya0Kazuichi Maruyama1Sunao Sugita2Masayo Takahashi3Shunji Yokokura4Kota Sato5Yasuhiro Tomaru6Norio Shimizu7Toru Nakazawa8Department of Ophthalmology and Visual Science, Tohoku University Graduate School of MedicineDepartment of Ophthalmology and Visual Science, Tohoku University Graduate School of MedicineLaboratory for Retinal Regeneration, RIKEN Center for Developmental BiologyLaboratory for Retinal Regeneration, RIKEN Center for Developmental BiologyDepartment of Ophthalmology and Visual Science, Tohoku University Graduate School of MedicineDepartment of Ophthalmology and Visual Science, Tohoku University Graduate School of MedicineDepartment of Virology, Medical Research Institute, Tokyo Medical and Dental UniversityDepartment of Virology, Medical Research Institute, Tokyo Medical and Dental UniversityDepartment of Ophthalmology and Visual Science, Tohoku University Graduate School of MedicineAbstract Corneal transplantation is a safe, reliable method of restoring visual acuity in patients with corneal disorders. Although it has a very high success rate, rejection can still occur, especially if the site is infected. Therefore, seeking to find better ways to manage infection risk, this study investigated a new technique, based on multiplex polymerase chain reaction (mPCR), to identify pathogens, including viruses, bacteria, and fungi, in corneal transplantation recipient sites, donor corneas and the donor cornea storage solution. The subjects comprised 50 patients who underwent corneal transplantation at Tohoku University Hospital between July 2014 and April 2015. We obtained extracted (recipient) cornea samples in 37 cases, donor cornea samples in 50 cases, and corneal storage solution samples in 50 cases (18 of these 50 samples contained DNA). Herpes simplex virus type 1 DNA was detected in four recipient corneas, Parvovirus B19 DNA was detected in two recipient corneas, Human herpes virus type 6 was detected in two donor corneas, and Aspergillus DNA was detected in one corneal storage solution sample. Thus, mPCR successfully identified pathogenic DNA in corneal tissues and storage solution, suggesting that evaluation with mPCR may improve the ability to predict the risk of infection after corneal transplantation.https://doi.org/10.1038/s41598-017-06344-3 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Takehiro Hariya Kazuichi Maruyama Sunao Sugita Masayo Takahashi Shunji Yokokura Kota Sato Yasuhiro Tomaru Norio Shimizu Toru Nakazawa |
spellingShingle |
Takehiro Hariya Kazuichi Maruyama Sunao Sugita Masayo Takahashi Shunji Yokokura Kota Sato Yasuhiro Tomaru Norio Shimizu Toru Nakazawa Multiplex polymerase chain reaction for pathogen detection in donor/recipient corneal transplant tissue and donor storage solution Scientific Reports |
author_facet |
Takehiro Hariya Kazuichi Maruyama Sunao Sugita Masayo Takahashi Shunji Yokokura Kota Sato Yasuhiro Tomaru Norio Shimizu Toru Nakazawa |
author_sort |
Takehiro Hariya |
title |
Multiplex polymerase chain reaction for pathogen detection in donor/recipient corneal transplant tissue and donor storage solution |
title_short |
Multiplex polymerase chain reaction for pathogen detection in donor/recipient corneal transplant tissue and donor storage solution |
title_full |
Multiplex polymerase chain reaction for pathogen detection in donor/recipient corneal transplant tissue and donor storage solution |
title_fullStr |
Multiplex polymerase chain reaction for pathogen detection in donor/recipient corneal transplant tissue and donor storage solution |
title_full_unstemmed |
Multiplex polymerase chain reaction for pathogen detection in donor/recipient corneal transplant tissue and donor storage solution |
title_sort |
multiplex polymerase chain reaction for pathogen detection in donor/recipient corneal transplant tissue and donor storage solution |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2017-07-01 |
description |
Abstract Corneal transplantation is a safe, reliable method of restoring visual acuity in patients with corneal disorders. Although it has a very high success rate, rejection can still occur, especially if the site is infected. Therefore, seeking to find better ways to manage infection risk, this study investigated a new technique, based on multiplex polymerase chain reaction (mPCR), to identify pathogens, including viruses, bacteria, and fungi, in corneal transplantation recipient sites, donor corneas and the donor cornea storage solution. The subjects comprised 50 patients who underwent corneal transplantation at Tohoku University Hospital between July 2014 and April 2015. We obtained extracted (recipient) cornea samples in 37 cases, donor cornea samples in 50 cases, and corneal storage solution samples in 50 cases (18 of these 50 samples contained DNA). Herpes simplex virus type 1 DNA was detected in four recipient corneas, Parvovirus B19 DNA was detected in two recipient corneas, Human herpes virus type 6 was detected in two donor corneas, and Aspergillus DNA was detected in one corneal storage solution sample. Thus, mPCR successfully identified pathogenic DNA in corneal tissues and storage solution, suggesting that evaluation with mPCR may improve the ability to predict the risk of infection after corneal transplantation. |
url |
https://doi.org/10.1038/s41598-017-06344-3 |
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