GSH Modification as a Marker for Plasma Source and Biological Response Comparison to Plasma Treatment
This study investigated the use of glutathione as a marker to establish a correlation between plasma parameters and the resultant liquid chemistry from two distinct sources to predefined biological outcomes. Two different plasma sources were operated at parameters that resulted in similar biological...
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doaj-153556d6d2ce420b8fcf087bd0d94eb52020-11-25T03:50:59ZengMDPI AGApplied Sciences2076-34172020-03-01106202510.3390/app10062025app10062025GSH Modification as a Marker for Plasma Source and Biological Response Comparison to Plasma TreatmentPietro Ranieri0Hager Mohamed1Brayden Myers2Leah Dobossy3Keely Beyries4Duncan Trosan5Fred C. Krebs6Vandana Miller7Katharina Stapelmann8Department of Nuclear Engineering, North Carolina State University, Raleigh, NC 27605, USADepartment of Microbiology and Immunology, and Institute for Molecular Medicine & Infectious Disease, Drexel University College of Medicine, Philadelphia, PA 19102, USADepartment of Nuclear Engineering, North Carolina State University, Raleigh, NC 27605, USADepartment of Microbiology and Immunology, and Institute for Molecular Medicine & Infectious Disease, Drexel University College of Medicine, Philadelphia, PA 19102, USADepartment of Microbiology and Immunology, and Institute for Molecular Medicine & Infectious Disease, Drexel University College of Medicine, Philadelphia, PA 19102, USADepartment of Nuclear Engineering, North Carolina State University, Raleigh, NC 27605, USADepartment of Microbiology and Immunology, and Institute for Molecular Medicine & Infectious Disease, Drexel University College of Medicine, Philadelphia, PA 19102, USADepartment of Microbiology and Immunology, and Institute for Molecular Medicine & Infectious Disease, Drexel University College of Medicine, Philadelphia, PA 19102, USADepartment of Nuclear Engineering, North Carolina State University, Raleigh, NC 27605, USAThis study investigated the use of glutathione as a marker to establish a correlation between plasma parameters and the resultant liquid chemistry from two distinct sources to predefined biological outcomes. Two different plasma sources were operated at parameters that resulted in similar biological responses: cell viability, mitochondrial activity, and the cell surface display of calreticulin. Specific glutathione modifications appeared to be associated with biological responses elicited by plasma. These modifications were more pronounced with increased treatment time for the European Cooperation in Science and Technology Reference Microplasma Jet (COST-Jet) and increased frequency for the dielectric barrier discharge and were correlated with more potent biological responses. No correlations were found when cells or glutathione were exposed to exogenously added long-lived species alone. This implied that short-lived species and other plasma components were required for the induction of cellular responses, as well as glutathione modifications. These results showed that comparisons of medical plasma sources could not rely on measurements of long-lived chemical species; rather, modifications of biomolecules (such as glutathione) might be better predictors of cellular responses to plasma exposure.https://www.mdpi.com/2076-3417/10/6/2025cost-jetnspdbdatmospheric pressure plasmaslow-temperature plasmasplasma chemistry |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Pietro Ranieri Hager Mohamed Brayden Myers Leah Dobossy Keely Beyries Duncan Trosan Fred C. Krebs Vandana Miller Katharina Stapelmann |
spellingShingle |
Pietro Ranieri Hager Mohamed Brayden Myers Leah Dobossy Keely Beyries Duncan Trosan Fred C. Krebs Vandana Miller Katharina Stapelmann GSH Modification as a Marker for Plasma Source and Biological Response Comparison to Plasma Treatment Applied Sciences cost-jet nspdbd atmospheric pressure plasmas low-temperature plasmas plasma chemistry |
author_facet |
Pietro Ranieri Hager Mohamed Brayden Myers Leah Dobossy Keely Beyries Duncan Trosan Fred C. Krebs Vandana Miller Katharina Stapelmann |
author_sort |
Pietro Ranieri |
title |
GSH Modification as a Marker for Plasma Source and Biological Response Comparison to Plasma Treatment |
title_short |
GSH Modification as a Marker for Plasma Source and Biological Response Comparison to Plasma Treatment |
title_full |
GSH Modification as a Marker for Plasma Source and Biological Response Comparison to Plasma Treatment |
title_fullStr |
GSH Modification as a Marker for Plasma Source and Biological Response Comparison to Plasma Treatment |
title_full_unstemmed |
GSH Modification as a Marker for Plasma Source and Biological Response Comparison to Plasma Treatment |
title_sort |
gsh modification as a marker for plasma source and biological response comparison to plasma treatment |
publisher |
MDPI AG |
series |
Applied Sciences |
issn |
2076-3417 |
publishDate |
2020-03-01 |
description |
This study investigated the use of glutathione as a marker to establish a correlation between plasma parameters and the resultant liquid chemistry from two distinct sources to predefined biological outcomes. Two different plasma sources were operated at parameters that resulted in similar biological responses: cell viability, mitochondrial activity, and the cell surface display of calreticulin. Specific glutathione modifications appeared to be associated with biological responses elicited by plasma. These modifications were more pronounced with increased treatment time for the European Cooperation in Science and Technology Reference Microplasma Jet (COST-Jet) and increased frequency for the dielectric barrier discharge and were correlated with more potent biological responses. No correlations were found when cells or glutathione were exposed to exogenously added long-lived species alone. This implied that short-lived species and other plasma components were required for the induction of cellular responses, as well as glutathione modifications. These results showed that comparisons of medical plasma sources could not rely on measurements of long-lived chemical species; rather, modifications of biomolecules (such as glutathione) might be better predictors of cellular responses to plasma exposure. |
topic |
cost-jet nspdbd atmospheric pressure plasmas low-temperature plasmas plasma chemistry |
url |
https://www.mdpi.com/2076-3417/10/6/2025 |
work_keys_str_mv |
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