Dehydroabietic Acid Is a Novel Survivin Inhibitor for Gastric Cancer
Gastric cancer is a malignant tumor with a high incidence and mortality rate worldwide. Nevertheless, anticancer drugs that can be used for gastric cancer treatment are limited. Therefore, it is important to develop targeted anticancer drugs for the treatment of gastric cancer. Dehydroabietic acid (...
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doaj-1557726058d44f77b98b158bfc58b07e2021-06-01T00:47:48ZengMDPI AGPlants2223-77472021-05-01101047104710.3390/plants10061047Dehydroabietic Acid Is a Novel Survivin Inhibitor for Gastric CancerWon-Jin Kim0Woong Kim1Jang-Mi Bae2Jungsoo Gim3Seok-Jun Kim4Department of Integrative Biological Sciences, Chosun University, Gwangju 61452, KoreaBK21 FOUR Educational Research Group for Age-Associated Disorder Control Technology, Chosun University, Gwangju 61452, KoreaDepartment of Integrative Biological Sciences, Chosun University, Gwangju 61452, KoreaDepartment of Integrative Biological Sciences, Chosun University, Gwangju 61452, KoreaDepartment of Integrative Biological Sciences, Chosun University, Gwangju 61452, KoreaGastric cancer is a malignant tumor with a high incidence and mortality rate worldwide. Nevertheless, anticancer drugs that can be used for gastric cancer treatment are limited. Therefore, it is important to develop targeted anticancer drugs for the treatment of gastric cancer. Dehydroabietic acid (DAA) is a diterpene found in tree pine. Previous studies have demonstrated that DAA inhibits gastric cancer cell proliferation by inducing apoptosis. However, we did not know how DAA inhibits the proliferation of gastric cancer cells through apoptosis. In this study, we attempted to identify the genes that induce cell cycle arrest and cell death, as well as those which are altered by DAA treatment. DAA-regulated genes were screened using RNA-Seq and differentially expressed genes (DEGs) analysis in AGS cells. RNA-Seq analysis revealed that the expression of survivin, an apoptosis inhibitor, was significantly reduced by DAA treatment. We also confirmed that DAA decreased survivin expression by RT-PCR and Western blotting analysis. In addition, the ability of DAA to inhibit survivin was compared to that of YM-155, a known survivin inhibitor. DAA was found to have a stronger inhibitory effect in comparison with YM-155. DAA also caused an increase in cleaved caspase-3, an apoptosis-activating protein. In conclusion, DAA is a potential anticancer agent for gastric cancer that inhibits survivin expression.https://www.mdpi.com/2223-7747/10/6/1047dehydroabietic acidgastric cancersurvivin |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Won-Jin Kim Woong Kim Jang-Mi Bae Jungsoo Gim Seok-Jun Kim |
spellingShingle |
Won-Jin Kim Woong Kim Jang-Mi Bae Jungsoo Gim Seok-Jun Kim Dehydroabietic Acid Is a Novel Survivin Inhibitor for Gastric Cancer Plants dehydroabietic acid gastric cancer survivin |
author_facet |
Won-Jin Kim Woong Kim Jang-Mi Bae Jungsoo Gim Seok-Jun Kim |
author_sort |
Won-Jin Kim |
title |
Dehydroabietic Acid Is a Novel Survivin Inhibitor for Gastric Cancer |
title_short |
Dehydroabietic Acid Is a Novel Survivin Inhibitor for Gastric Cancer |
title_full |
Dehydroabietic Acid Is a Novel Survivin Inhibitor for Gastric Cancer |
title_fullStr |
Dehydroabietic Acid Is a Novel Survivin Inhibitor for Gastric Cancer |
title_full_unstemmed |
Dehydroabietic Acid Is a Novel Survivin Inhibitor for Gastric Cancer |
title_sort |
dehydroabietic acid is a novel survivin inhibitor for gastric cancer |
publisher |
MDPI AG |
series |
Plants |
issn |
2223-7747 |
publishDate |
2021-05-01 |
description |
Gastric cancer is a malignant tumor with a high incidence and mortality rate worldwide. Nevertheless, anticancer drugs that can be used for gastric cancer treatment are limited. Therefore, it is important to develop targeted anticancer drugs for the treatment of gastric cancer. Dehydroabietic acid (DAA) is a diterpene found in tree pine. Previous studies have demonstrated that DAA inhibits gastric cancer cell proliferation by inducing apoptosis. However, we did not know how DAA inhibits the proliferation of gastric cancer cells through apoptosis. In this study, we attempted to identify the genes that induce cell cycle arrest and cell death, as well as those which are altered by DAA treatment. DAA-regulated genes were screened using RNA-Seq and differentially expressed genes (DEGs) analysis in AGS cells. RNA-Seq analysis revealed that the expression of survivin, an apoptosis inhibitor, was significantly reduced by DAA treatment. We also confirmed that DAA decreased survivin expression by RT-PCR and Western blotting analysis. In addition, the ability of DAA to inhibit survivin was compared to that of YM-155, a known survivin inhibitor. DAA was found to have a stronger inhibitory effect in comparison with YM-155. DAA also caused an increase in cleaved caspase-3, an apoptosis-activating protein. In conclusion, DAA is a potential anticancer agent for gastric cancer that inhibits survivin expression. |
topic |
dehydroabietic acid gastric cancer survivin |
url |
https://www.mdpi.com/2223-7747/10/6/1047 |
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