Amy2B copy number variation reveals starch diet adaptations in ancient European dogs
Extant dog and wolf DNA indicates that dog domestication was accompanied by the selection of a series of duplications on the Amy2B gene coding for pancreatic amylase. In this study, we used a palaeogenetic approach to investigate the timing and expansion of the Amy2B gene in the ancient dog populati...
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The Royal Society
2016-01-01
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Online Access: | https://royalsocietypublishing.org/doi/pdf/10.1098/rsos.160449 |
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doaj-15700dbf445246328950dafc6279240d2020-11-25T03:57:37ZengThe Royal SocietyRoyal Society Open Science2054-57032016-01-0131110.1098/rsos.160449160449Amy2B copy number variation reveals starch diet adaptations in ancient European dogsMorgane OllivierAnne TressetFabiola BastianLaetitia LagoutteErik AxelssonMaja-Louise ArendtAdrian BălăşescuMarjan MarshourMikhail V. SablinLaure SalanovaJean-Denis VigneChristophe HitteCatherine HänniExtant dog and wolf DNA indicates that dog domestication was accompanied by the selection of a series of duplications on the Amy2B gene coding for pancreatic amylase. In this study, we used a palaeogenetic approach to investigate the timing and expansion of the Amy2B gene in the ancient dog populations of Western and Eastern Europe and Southwest Asia. Quantitative polymerase chain reaction was used to estimate the copy numbers of this gene for 13 ancient dog samples, dated to between 15 000 and 4000 years before present (cal. BP). This evidenced an increase of Amy2B copies in ancient dogs from as early as the 7th millennium cal. BP in Southeastern Europe. We found that the gene expansion was not fixed across all dogs within this early farming context, with ancient dogs bearing between 2 and 20 diploid copies of the gene. The results also suggested that selection for the increased Amy2B copy number started 7000 years cal. BP, at the latest. This expansion reflects a local adaptation that allowed dogs to thrive on a starch rich diet, especially within early farming societies, and suggests a biocultural coevolution of dog genes and human culture.https://royalsocietypublishing.org/doi/pdf/10.1098/rsos.160449domesticationpalaeogenomicsamylasedogneolithic |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Morgane Ollivier Anne Tresset Fabiola Bastian Laetitia Lagoutte Erik Axelsson Maja-Louise Arendt Adrian Bălăşescu Marjan Marshour Mikhail V. Sablin Laure Salanova Jean-Denis Vigne Christophe Hitte Catherine Hänni |
spellingShingle |
Morgane Ollivier Anne Tresset Fabiola Bastian Laetitia Lagoutte Erik Axelsson Maja-Louise Arendt Adrian Bălăşescu Marjan Marshour Mikhail V. Sablin Laure Salanova Jean-Denis Vigne Christophe Hitte Catherine Hänni Amy2B copy number variation reveals starch diet adaptations in ancient European dogs Royal Society Open Science domestication palaeogenomics amylase dog neolithic |
author_facet |
Morgane Ollivier Anne Tresset Fabiola Bastian Laetitia Lagoutte Erik Axelsson Maja-Louise Arendt Adrian Bălăşescu Marjan Marshour Mikhail V. Sablin Laure Salanova Jean-Denis Vigne Christophe Hitte Catherine Hänni |
author_sort |
Morgane Ollivier |
title |
Amy2B copy number variation reveals starch diet adaptations in ancient European dogs |
title_short |
Amy2B copy number variation reveals starch diet adaptations in ancient European dogs |
title_full |
Amy2B copy number variation reveals starch diet adaptations in ancient European dogs |
title_fullStr |
Amy2B copy number variation reveals starch diet adaptations in ancient European dogs |
title_full_unstemmed |
Amy2B copy number variation reveals starch diet adaptations in ancient European dogs |
title_sort |
amy2b copy number variation reveals starch diet adaptations in ancient european dogs |
publisher |
The Royal Society |
series |
Royal Society Open Science |
issn |
2054-5703 |
publishDate |
2016-01-01 |
description |
Extant dog and wolf DNA indicates that dog domestication was accompanied by the selection of a series of duplications on the Amy2B gene coding for pancreatic amylase. In this study, we used a palaeogenetic approach to investigate the timing and expansion of the Amy2B gene in the ancient dog populations of Western and Eastern Europe and Southwest Asia. Quantitative polymerase chain reaction was used to estimate the copy numbers of this gene for 13 ancient dog samples, dated to between 15 000 and 4000 years before present (cal. BP). This evidenced an increase of Amy2B copies in ancient dogs from as early as the 7th millennium cal. BP in Southeastern Europe. We found that the gene expansion was not fixed across all dogs within this early farming context, with ancient dogs bearing between 2 and 20 diploid copies of the gene. The results also suggested that selection for the increased Amy2B copy number started 7000 years cal. BP, at the latest. This expansion reflects a local adaptation that allowed dogs to thrive on a starch rich diet, especially within early farming societies, and suggests a biocultural coevolution of dog genes and human culture. |
topic |
domestication palaeogenomics amylase dog neolithic |
url |
https://royalsocietypublishing.org/doi/pdf/10.1098/rsos.160449 |
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