Hypoglycemic Efficacy of Docking Selected Natural Compounds against α-Glucosidase and α-Amylase
The inhibition of α-glucosidase and α-amylase is a clinical strategy for the treatment of type II diabetes, and herbal medicines have been reported to credibly alleviate hyperglycemia. Our previous study has reported some constituents from plant or herbal sources targeted to &...
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doaj-1583fcfceff64cf9a9730180e5bc9d832020-11-24T22:20:07ZengMDPI AGMolecules1420-30492018-09-01239226010.3390/molecules23092260molecules23092260Hypoglycemic Efficacy of Docking Selected Natural Compounds against α-Glucosidase and α-AmylaseJirawat Riyaphan0Chien-Hung Jhong1Shian-Ren Lin2Chia-Hsiang Chang3May-Jwan Tsai4Der-Nan Lee5Ping-Jyun Sung6Max K. Leong7Ching-Feng Weng8Department of Life Science and Institute of Biotechnology, National Dong-Hwa University, Hualien 97401, TaiwanDepartment of Life Science and Institute of Biotechnology, National Dong-Hwa University, Hualien 97401, TaiwanDepartment of Life Science and Institute of Biotechnology, National Dong-Hwa University, Hualien 97401, TaiwanDepartment of Life Science and Institute of Biotechnology, National Dong-Hwa University, Hualien 97401, TaiwanNeural Regeneration Laboratory, Neurological Institute, Taipei Veterans General Hospital, Taipei 11217, TaiwanDepartment of Biotechnology and Animal Science, National Ilan University, Ilan 26047, TaiwanNational Museum of Marine Biology and Aquarium, Pingtung 94450, TaiwanDepartment of Life Science and Institute of Biotechnology, National Dong-Hwa University, Hualien 97401, TaiwanDepartment of Life Science and Institute of Biotechnology, National Dong-Hwa University, Hualien 97401, TaiwanThe inhibition of α-glucosidase and α-amylase is a clinical strategy for the treatment of type II diabetes, and herbal medicines have been reported to credibly alleviate hyperglycemia. Our previous study has reported some constituents from plant or herbal sources targeted to α-glucosidase and α-amylase via molecular docking and enzymatic measurement, but the hypoglycemic potencies in cell system and mice have not been validated yet. This study was aimed to elucidate the hypoglycemic efficacy of docking selected compounds in cell assay and oral glucose and starch tolerance tests of mice. All test compounds showed the inhibition of α-glucosidase activity in Caco-2 cells. The decrease of blood sugar levels of test compounds in 30 min and 60 min of mice after OGTT and OSTT, respectively and the decreased glucose levels of test compounds were significantly varied in acarbose. Taken altogether, in vitro and in vivo experiments suggest that selected natural compounds (curcumin, antroquinonol, HCD, docosanol, tetracosanol, rutin, and actinodaphnine) via molecular docking were confirmed as potential candidates of α-glucosidase and α-amylase inhibitors for treating diabetes.http://www.mdpi.com/1420-3049/23/9/2260natural compoundα-glucosidaseα-amylase |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jirawat Riyaphan Chien-Hung Jhong Shian-Ren Lin Chia-Hsiang Chang May-Jwan Tsai Der-Nan Lee Ping-Jyun Sung Max K. Leong Ching-Feng Weng |
spellingShingle |
Jirawat Riyaphan Chien-Hung Jhong Shian-Ren Lin Chia-Hsiang Chang May-Jwan Tsai Der-Nan Lee Ping-Jyun Sung Max K. Leong Ching-Feng Weng Hypoglycemic Efficacy of Docking Selected Natural Compounds against α-Glucosidase and α-Amylase Molecules natural compound α-glucosidase α-amylase |
author_facet |
Jirawat Riyaphan Chien-Hung Jhong Shian-Ren Lin Chia-Hsiang Chang May-Jwan Tsai Der-Nan Lee Ping-Jyun Sung Max K. Leong Ching-Feng Weng |
author_sort |
Jirawat Riyaphan |
title |
Hypoglycemic Efficacy of Docking Selected Natural Compounds against α-Glucosidase and α-Amylase |
title_short |
Hypoglycemic Efficacy of Docking Selected Natural Compounds against α-Glucosidase and α-Amylase |
title_full |
Hypoglycemic Efficacy of Docking Selected Natural Compounds against α-Glucosidase and α-Amylase |
title_fullStr |
Hypoglycemic Efficacy of Docking Selected Natural Compounds against α-Glucosidase and α-Amylase |
title_full_unstemmed |
Hypoglycemic Efficacy of Docking Selected Natural Compounds against α-Glucosidase and α-Amylase |
title_sort |
hypoglycemic efficacy of docking selected natural compounds against α-glucosidase and α-amylase |
publisher |
MDPI AG |
series |
Molecules |
issn |
1420-3049 |
publishDate |
2018-09-01 |
description |
The inhibition of α-glucosidase and α-amylase is a clinical strategy for the treatment of type II diabetes, and herbal medicines have been reported to credibly alleviate hyperglycemia. Our previous study has reported some constituents from plant or herbal sources targeted to α-glucosidase and α-amylase via molecular docking and enzymatic measurement, but the hypoglycemic potencies in cell system and mice have not been validated yet. This study was aimed to elucidate the hypoglycemic efficacy of docking selected compounds in cell assay and oral glucose and starch tolerance tests of mice. All test compounds showed the inhibition of α-glucosidase activity in Caco-2 cells. The decrease of blood sugar levels of test compounds in 30 min and 60 min of mice after OGTT and OSTT, respectively and the decreased glucose levels of test compounds were significantly varied in acarbose. Taken altogether, in vitro and in vivo experiments suggest that selected natural compounds (curcumin, antroquinonol, HCD, docosanol, tetracosanol, rutin, and actinodaphnine) via molecular docking were confirmed as potential candidates of α-glucosidase and α-amylase inhibitors for treating diabetes. |
topic |
natural compound α-glucosidase α-amylase |
url |
http://www.mdpi.com/1420-3049/23/9/2260 |
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