Strongyloides venezuelensis alkaline extract for the diagnosis of human strongyloidiasis by enzyme-linked immunosorbent assay

• Main Authors: Machado Eleuza Rodrigues, Ueta Marlene Tiduko, Gonçalves-Pires Maria do Rosário de Fátima, Oliveira João Batista Alves de, Faccioli Lúcia Helena, Costa-Cruz Julia Maria
• Format: Article
• Language: English
• Published: Instituto Oswaldo Cruz, Ministério da Saúde 2003-01-01
• Series: Memórias do Instituto Oswaldo Cruz.
• Subjects: Strongyloides venezuelensis; strongyloidiasis; diagnosis; enzyme-linked immunosorbent assay
• Online Access: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762003000600024
• ISSN: 0074-0276; 1678-8060

The present study was conducted to detected IgG antibodies using Strongyloides venezuelensis alkaline extract for the diagnosis of human strongyloidiasis by the enzyme-linked immunosorbent assay (ELISA). Sera from 90 subjects were analyzed (30 with strongyloidiasis, 30 with other parasites and 30 healthy individuals). Results were expressed in antibody titers, which were considered as positive when titer was > 80. Sensibility and specificity of the assay were 100% and 96.7%, respectively. It can be concluded that the heterologous alkaline extract could be employed in ELISA as a diagnostic aid in human strongyloidiasis, due to its advantages as easiness of obtaining, practicability in preparing, and high indexes of sensitivity and specificity.