Effects of Nogo-A Silencing on TNF-α and IL-6 Secretion and TH Downregulation in Lipopolysaccharide-Stimulated PC12 Cells
Parkinson’s disease (PD) is a common degenerative disease that lacks efficient treatment. Myelin-associated neurite outgrowth inhibitor A (Nogo-A) is relevant with inhibition of nerve regeneration and may play vital role in pathogenesis of PD. The study aimed to establish the shRNA expression plasmi...
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doaj-159c2658c2a84a68855ba9a236af9d9e2020-11-24T21:01:15ZengHindawi LimitedBioMed Research International2314-61332314-61412015-01-01201510.1155/2015/817914817914Effects of Nogo-A Silencing on TNF-α and IL-6 Secretion and TH Downregulation in Lipopolysaccharide-Stimulated PC12 CellsJianbin Zhong0Shengnuo Fan1Zhenwen Yan2Songhua Xiao3Limei Wan4Chibang Chen5Simin Zhong6Lu Liu7Jun Liu8Neurology Department, People’s Hospital of Zengcheng City (Boji Hospital Affiliated to Sun Yat-sen University), Guangzhou 511300, ChinaNeurology Department, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, ChinaNeurology Department, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, ChinaNeurology Department, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, ChinaNeurology Department, People’s Hospital of Zengcheng City (Boji Hospital Affiliated to Sun Yat-sen University), Guangzhou 511300, ChinaNeurology Department, People’s Hospital of Zengcheng City (Boji Hospital Affiliated to Sun Yat-sen University), Guangzhou 511300, ChinaNeurology Department, People’s Hospital of Zengcheng City (Boji Hospital Affiliated to Sun Yat-sen University), Guangzhou 511300, ChinaNeurology Department, People’s Hospital of Zengcheng City (Boji Hospital Affiliated to Sun Yat-sen University), Guangzhou 511300, ChinaNeurology Department, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, ChinaParkinson’s disease (PD) is a common degenerative disease that lacks efficient treatment. Myelin-associated neurite outgrowth inhibitor A (Nogo-A) is relevant with inhibition of nerve regeneration and may play vital role in pathogenesis of PD. The study aimed to establish the shRNA expression plasmids of Nogo-A gene and explore the regulatory effects of Nogo-A silencing on the expression of inflammation factor tumor necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6) as well as tyrosine hydroxylase (TH) in lipopolysaccharide- (LPS-) stimulated rat PC12 cells. The results showed that both mRNA and protein levels of Nogo-A in pGenesil-nogoA-shRNA group were downregulated. The viabilities of PC12 cells decreased with increase of LPS concentrations. LPS significantly increased the supernatant TNF-alpha and IL-6 concentrations and reduced TH protein expression in PC12 cells, while silencing Nogo-A could block these effects. These results suggested that LPS can activate PC12 cells to secrete inflammatory cytokines and lower the TH expression, which can be regulated by Nogo-A gene silencing. Nogo-A silencing might provide new ideas for PD treatment in the future.http://dx.doi.org/10.1155/2015/817914 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jianbin Zhong Shengnuo Fan Zhenwen Yan Songhua Xiao Limei Wan Chibang Chen Simin Zhong Lu Liu Jun Liu |
spellingShingle |
Jianbin Zhong Shengnuo Fan Zhenwen Yan Songhua Xiao Limei Wan Chibang Chen Simin Zhong Lu Liu Jun Liu Effects of Nogo-A Silencing on TNF-α and IL-6 Secretion and TH Downregulation in Lipopolysaccharide-Stimulated PC12 Cells BioMed Research International |
author_facet |
Jianbin Zhong Shengnuo Fan Zhenwen Yan Songhua Xiao Limei Wan Chibang Chen Simin Zhong Lu Liu Jun Liu |
author_sort |
Jianbin Zhong |
title |
Effects of Nogo-A Silencing on TNF-α and IL-6 Secretion and TH Downregulation in Lipopolysaccharide-Stimulated PC12 Cells |
title_short |
Effects of Nogo-A Silencing on TNF-α and IL-6 Secretion and TH Downregulation in Lipopolysaccharide-Stimulated PC12 Cells |
title_full |
Effects of Nogo-A Silencing on TNF-α and IL-6 Secretion and TH Downregulation in Lipopolysaccharide-Stimulated PC12 Cells |
title_fullStr |
Effects of Nogo-A Silencing on TNF-α and IL-6 Secretion and TH Downregulation in Lipopolysaccharide-Stimulated PC12 Cells |
title_full_unstemmed |
Effects of Nogo-A Silencing on TNF-α and IL-6 Secretion and TH Downregulation in Lipopolysaccharide-Stimulated PC12 Cells |
title_sort |
effects of nogo-a silencing on tnf-α and il-6 secretion and th downregulation in lipopolysaccharide-stimulated pc12 cells |
publisher |
Hindawi Limited |
series |
BioMed Research International |
issn |
2314-6133 2314-6141 |
publishDate |
2015-01-01 |
description |
Parkinson’s disease (PD) is a common degenerative disease that lacks efficient treatment. Myelin-associated neurite outgrowth inhibitor A (Nogo-A) is relevant with inhibition of nerve regeneration and may play vital role in pathogenesis of PD. The study aimed to establish the shRNA expression plasmids of Nogo-A gene and explore the regulatory effects of Nogo-A silencing on the expression of inflammation factor tumor necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6) as well as tyrosine hydroxylase (TH) in lipopolysaccharide- (LPS-) stimulated rat PC12 cells. The results showed that both mRNA and protein levels of Nogo-A in pGenesil-nogoA-shRNA group were downregulated. The viabilities of PC12 cells decreased with increase of LPS concentrations. LPS significantly increased the supernatant TNF-alpha and IL-6 concentrations and reduced TH protein expression in PC12 cells, while silencing Nogo-A could block these effects. These results suggested that LPS can activate PC12 cells to secrete inflammatory cytokines and lower the TH expression, which can be regulated by Nogo-A gene silencing. Nogo-A silencing might provide new ideas for PD treatment in the future. |
url |
http://dx.doi.org/10.1155/2015/817914 |
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