Purification and Characterization of a Thermo- and pH-Stable Laccase From the Litter-Decomposing Fungus Gymnopus luxurians and Laccase Mediator Systems for Dye Decolorization
An extracellular laccase (GLL) was purified from fermentation broth of the litter-decomposing fungus Gymnopus luxurians by four chromatography steps, which resulted in a high specific activity of 118.82 U/mg, purification fold of 41.22, and recovery rate of 42.05%. It is a monomeric protein with a m...
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Frontiers Media S.A.
2021-08-01
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Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2021.672620/full |
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record_format |
Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yue Sun Yue Sun Yue Sun Zi-Lu Liu Bo-Yang Hu Qing-Jun Chen Ai-Zhen Yang Qiu-Ying Wang Xiao-Feng Li Jia-Yan Zhang Guo-Qing Zhang Yong-Chang Zhao |
spellingShingle |
Yue Sun Yue Sun Yue Sun Zi-Lu Liu Bo-Yang Hu Qing-Jun Chen Ai-Zhen Yang Qiu-Ying Wang Xiao-Feng Li Jia-Yan Zhang Guo-Qing Zhang Yong-Chang Zhao Purification and Characterization of a Thermo- and pH-Stable Laccase From the Litter-Decomposing Fungus Gymnopus luxurians and Laccase Mediator Systems for Dye Decolorization Frontiers in Microbiology Gymnopus luxurians laccase laccase mediator system dye decolorization purification |
author_facet |
Yue Sun Yue Sun Yue Sun Zi-Lu Liu Bo-Yang Hu Qing-Jun Chen Ai-Zhen Yang Qiu-Ying Wang Xiao-Feng Li Jia-Yan Zhang Guo-Qing Zhang Yong-Chang Zhao |
author_sort |
Yue Sun |
title |
Purification and Characterization of a Thermo- and pH-Stable Laccase From the Litter-Decomposing Fungus Gymnopus luxurians and Laccase Mediator Systems for Dye Decolorization |
title_short |
Purification and Characterization of a Thermo- and pH-Stable Laccase From the Litter-Decomposing Fungus Gymnopus luxurians and Laccase Mediator Systems for Dye Decolorization |
title_full |
Purification and Characterization of a Thermo- and pH-Stable Laccase From the Litter-Decomposing Fungus Gymnopus luxurians and Laccase Mediator Systems for Dye Decolorization |
title_fullStr |
Purification and Characterization of a Thermo- and pH-Stable Laccase From the Litter-Decomposing Fungus Gymnopus luxurians and Laccase Mediator Systems for Dye Decolorization |
title_full_unstemmed |
Purification and Characterization of a Thermo- and pH-Stable Laccase From the Litter-Decomposing Fungus Gymnopus luxurians and Laccase Mediator Systems for Dye Decolorization |
title_sort |
purification and characterization of a thermo- and ph-stable laccase from the litter-decomposing fungus gymnopus luxurians and laccase mediator systems for dye decolorization |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Microbiology |
issn |
1664-302X |
publishDate |
2021-08-01 |
description |
An extracellular laccase (GLL) was purified from fermentation broth of the litter-decomposing fungus Gymnopus luxurians by four chromatography steps, which resulted in a high specific activity of 118.82 U/mg, purification fold of 41.22, and recovery rate of 42.05%. It is a monomeric protein with a molecular weight of 64 kDa and N-terminal amino acid sequence of AIGPV TDLHI, suggesting that GLL is a typical fungal laccase. GLL demonstrated an optimum temperature range of 55°C–65°C and an optimum pH 2.2 toward 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS). It displayed considerably high thermostability and pH stability with about 63% activity retained after 24 h at 50°C, and 86% activity retained after 24 h at pH 2.2, respectively. GLL was significantly enhanced in the presence of K+, Na+, and Mg2+ ions. It demonstrated Km of 539 μM and kcat/Km of 140 mM–1⋅s–1 toward ABTS at pH 2.2 and 37°C. Acetosyringone (AS) and syringaldehyde (SA) were the optimal mediators of GLL (0.4 U/ml) for dye decolorization with decolorization rates of about 60%–90% toward 11 of the 14 synthetic dyes. The optimum reaction conditions were determined to be mediator concentration of 0.1 mM, temperature range of 25°C –60°C, and pH 4.0. The purified laccase was the first laccase isolated from genus Gymnopus with high thermostability, pH stability, and effective decolorization toward dyes, suggesting that it has potentials for textile and environmental applications. |
topic |
Gymnopus luxurians laccase laccase mediator system dye decolorization purification |
url |
https://www.frontiersin.org/articles/10.3389/fmicb.2021.672620/full |
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doaj-15bf02b8f6fa43ceb601ae10beb041df2021-08-03T07:51:50ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2021-08-011210.3389/fmicb.2021.672620672620Purification and Characterization of a Thermo- and pH-Stable Laccase From the Litter-Decomposing Fungus Gymnopus luxurians and Laccase Mediator Systems for Dye DecolorizationYue Sun0Yue Sun1Yue Sun2Zi-Lu Liu3Bo-Yang Hu4Qing-Jun Chen5Ai-Zhen Yang6Qiu-Ying Wang7Xiao-Feng Li8Jia-Yan Zhang9Guo-Qing Zhang10Yong-Chang Zhao11Beijing Key Laboratory for Agricultural Application and New Technique, Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Plant Science and Technology, Beijing University of Agriculture, Beijing, ChinaKey Laboratory for Northern Urban Agriculture of Ministry of Agriculture and Rural Affairs, College of Bioscience and Resources Environment, Beijing University of Agriculture, Beijing, ChinaCollege of Biological Sciences and Technology, Beijing Forestry University, Beijing, ChinaKey Laboratory for Northern Urban Agriculture of Ministry of Agriculture and Rural Affairs, College of Bioscience and Resources Environment, Beijing University of Agriculture, Beijing, ChinaKey Laboratory for Northern Urban Agriculture of Ministry of Agriculture and Rural Affairs, College of Bioscience and Resources Environment, Beijing University of Agriculture, Beijing, ChinaBeijing Key Laboratory for Agricultural Application and New Technique, Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Plant Science and Technology, Beijing University of Agriculture, Beijing, ChinaKey Laboratory for Northern Urban Agriculture of Ministry of Agriculture and Rural Affairs, College of Bioscience and Resources Environment, Beijing University of Agriculture, Beijing, ChinaKey Laboratory for Northern Urban Agriculture of Ministry of Agriculture and Rural Affairs, College of Bioscience and Resources Environment, Beijing University of Agriculture, Beijing, ChinaKey Laboratory for Northern Urban Agriculture of Ministry of Agriculture and Rural Affairs, College of Bioscience and Resources Environment, Beijing University of Agriculture, Beijing, ChinaKey Laboratory for Northern Urban Agriculture of Ministry of Agriculture and Rural Affairs, College of Bioscience and Resources Environment, Beijing University of Agriculture, Beijing, ChinaBeijing Key Laboratory for Agricultural Application and New Technique, Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Plant Science and Technology, Beijing University of Agriculture, Beijing, ChinaInstitute of Biotechnology and Germplasmic Resource, Yunnan Academy of Agricultural Sciences, Kunming, ChinaAn extracellular laccase (GLL) was purified from fermentation broth of the litter-decomposing fungus Gymnopus luxurians by four chromatography steps, which resulted in a high specific activity of 118.82 U/mg, purification fold of 41.22, and recovery rate of 42.05%. It is a monomeric protein with a molecular weight of 64 kDa and N-terminal amino acid sequence of AIGPV TDLHI, suggesting that GLL is a typical fungal laccase. GLL demonstrated an optimum temperature range of 55°C–65°C and an optimum pH 2.2 toward 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS). It displayed considerably high thermostability and pH stability with about 63% activity retained after 24 h at 50°C, and 86% activity retained after 24 h at pH 2.2, respectively. GLL was significantly enhanced in the presence of K+, Na+, and Mg2+ ions. It demonstrated Km of 539 μM and kcat/Km of 140 mM–1⋅s–1 toward ABTS at pH 2.2 and 37°C. Acetosyringone (AS) and syringaldehyde (SA) were the optimal mediators of GLL (0.4 U/ml) for dye decolorization with decolorization rates of about 60%–90% toward 11 of the 14 synthetic dyes. The optimum reaction conditions were determined to be mediator concentration of 0.1 mM, temperature range of 25°C –60°C, and pH 4.0. The purified laccase was the first laccase isolated from genus Gymnopus with high thermostability, pH stability, and effective decolorization toward dyes, suggesting that it has potentials for textile and environmental applications.https://www.frontiersin.org/articles/10.3389/fmicb.2021.672620/fullGymnopus luxurianslaccaselaccase mediator systemdye decolorizationpurification |