2HybridTools, a handy software to facilitate clone identification and mutation mapping from yeast two-hybrid screening

Yeast Two-Hybrid (Y2H) and reverse Two-Hybrid (RY2H) are powerful protein–protein interaction screening methods that rely on the interaction of bait and prey proteins fused to DNA binding (DB) and activation domains (AD), respectively. Y2H allows identification of protein interaction partners using...

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Bibliographic Details
Main Authors: Pierre Cauchy, Brigitte Kahn-Perlès, Pierre Ferrier, Jean Imbert, Patrick Lécine
Format: Article
Language:English
Published: PeerJ Inc. 2019-07-01
Series:PeerJ
Subjects:
Online Access:https://peerj.com/articles/7245.pdf