Physiological-biochemical parameters and characteristics of seed coat structure in lupin seeds subjected to long storage at different temperatures
Seed vigour, viability, the contents of soluble carbohydrates, total protein, albumins, and globulins, as well as seed coat structure, were analysed in yellow lupin (Lupinus luteus L.) cv. Iryd seeds stored for 20 years at -14oC, 0oC or at room temperature (approx. +20oC). Seed storage at room tempe...
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doaj-15ed3d6411104316aa8b7cb739469aa02020-11-25T03:28:46ZengPolish Botanical SocietyActa Societatis Botanicorum Poloniae2083-94802011-01-0177320120510.5586/asbp.2008.024245Physiological-biochemical parameters and characteristics of seed coat structure in lupin seeds subjected to long storage at different temperaturesAgnieszka I. Piotrowicz-Cieślak0Dariusz J. Michalczyk1Kamila Górska2Zofia Bulińska-Radomska3Ryszard J. Górecki4University of Warmia and Mazury in OlsztynUniversity of Warmia and Mazury in OlsztynUniversity of Warmia and Mazury in OlsztynPlant Breeding and Acclimatization InstituteUniversity of Warmia and Mazury in OlsztynSeed vigour, viability, the contents of soluble carbohydrates, total protein, albumins, and globulins, as well as seed coat structure, were analysed in yellow lupin (Lupinus luteus L.) cv. Iryd seeds stored for 20 years at -14oC, 0oC or at room temperature (approx. +20oC). Seed storage at room temperature reduced viability (to 2%) and increased seed leachate electroconductivity. Determinations of total proteins showed that protein content was significantly reduced in seeds stored at +20oC compared to the other storage regimens. Raffinose family oligosaccharides were the main soluble carbohydrates in seeds stored at 0oC and -14oC, whereas sucrose dominated in seeds stored at room temperature. Scanning electron microscopy (SEM) of seed surface and seed coat sections revealed appearance of an amorphic layer on the surface of seeds stored at room temperature (not observed in other seeds) and distinct shrinking of macrosclereid layer in seeds stored at -14oC. Macrosclereids layer in all seeds was 100 um thick and accounted for 60% of seed coat thickness. The obtained results suggest that for long term storage of lupin seeds at 0oC is the most advisable temperature if both costs of storage and seed storability are considered.https://pbsociety.org.pl/journals/index.php/asbp/article/view/437lupin seedsstorabilityproteinssoluble carbohydratesseed surfaceseed coat |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Agnieszka I. Piotrowicz-Cieślak Dariusz J. Michalczyk Kamila Górska Zofia Bulińska-Radomska Ryszard J. Górecki |
spellingShingle |
Agnieszka I. Piotrowicz-Cieślak Dariusz J. Michalczyk Kamila Górska Zofia Bulińska-Radomska Ryszard J. Górecki Physiological-biochemical parameters and characteristics of seed coat structure in lupin seeds subjected to long storage at different temperatures Acta Societatis Botanicorum Poloniae lupin seeds storability proteins soluble carbohydrates seed surface seed coat |
author_facet |
Agnieszka I. Piotrowicz-Cieślak Dariusz J. Michalczyk Kamila Górska Zofia Bulińska-Radomska Ryszard J. Górecki |
author_sort |
Agnieszka I. Piotrowicz-Cieślak |
title |
Physiological-biochemical parameters and characteristics of seed coat structure in lupin seeds subjected to long storage at different temperatures |
title_short |
Physiological-biochemical parameters and characteristics of seed coat structure in lupin seeds subjected to long storage at different temperatures |
title_full |
Physiological-biochemical parameters and characteristics of seed coat structure in lupin seeds subjected to long storage at different temperatures |
title_fullStr |
Physiological-biochemical parameters and characteristics of seed coat structure in lupin seeds subjected to long storage at different temperatures |
title_full_unstemmed |
Physiological-biochemical parameters and characteristics of seed coat structure in lupin seeds subjected to long storage at different temperatures |
title_sort |
physiological-biochemical parameters and characteristics of seed coat structure in lupin seeds subjected to long storage at different temperatures |
publisher |
Polish Botanical Society |
series |
Acta Societatis Botanicorum Poloniae |
issn |
2083-9480 |
publishDate |
2011-01-01 |
description |
Seed vigour, viability, the contents of soluble carbohydrates, total protein, albumins, and globulins, as well as seed coat structure, were analysed in yellow lupin (Lupinus luteus L.) cv. Iryd seeds stored for 20 years at -14oC, 0oC or at room temperature (approx. +20oC). Seed storage at room temperature reduced viability (to 2%) and increased seed leachate electroconductivity. Determinations of total proteins showed that protein content was significantly reduced in seeds stored at +20oC compared to the other storage regimens. Raffinose family oligosaccharides were the main soluble carbohydrates in seeds stored at 0oC and -14oC, whereas sucrose dominated in seeds stored at room temperature. Scanning electron microscopy (SEM) of seed surface and seed coat sections revealed appearance of an amorphic layer on the surface of seeds stored at room temperature (not observed in other seeds) and distinct shrinking of macrosclereid layer in seeds stored at -14oC. Macrosclereids layer in all seeds was 100 um thick and accounted for 60% of seed coat thickness. The obtained results suggest that for long term storage of lupin seeds at 0oC is the most advisable temperature if both costs of storage and seed storability are considered. |
topic |
lupin seeds storability proteins soluble carbohydrates seed surface seed coat |
url |
https://pbsociety.org.pl/journals/index.php/asbp/article/view/437 |
work_keys_str_mv |
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