A Combined Analysis of Gut and Skin Microbiota in Infants with Food Allergy and Atopic Dermatitis: A Pilot Study
The gut microbiota in patients with food allergy, and the skin microbiota in atopic dermatitis patients differ from those of healthy people. We hypothesize that relationships may exist between gut and skin microbiota in patients with allergies. The aim of this study was to determine the possible rel...
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doaj-161177b9a27c4bcd84e163367a4b551a2021-06-01T00:07:18ZengMDPI AGNutrients2072-66432021-05-01131682168210.3390/nu13051682A Combined Analysis of Gut and Skin Microbiota in Infants with Food Allergy and Atopic Dermatitis: A Pilot StudyEwa Łoś-Rycharska0Marcin Gołębiewski1Marcin Sikora2Tomasz Grzybowski3Marta Gorzkiewicz4Maria Popielarz5Julia Gawryjołek6Aneta Krogulska7Department of Pediatrics, Allergology and Gastroenterology, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun, 87-100 Toruń, PolandDepartment of Plant Physiology and Biotechnology, Nicolaus Copernicus University in Torun, 87-100 Toruń, PolandInterdisciplinary Centre of Modern Technologies, Nicolaus Copernicus University in Torun, 87-100 Toruń, PolandDepartment of Forensic Medicine, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun, 87-100 Toruń, PolandDepartment of Forensic Medicine, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun, 87-100 Toruń, PolandDepartment of Pediatrics, Allergology and Gastroenterology, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun, 87-100 Toruń, PolandDepartment of Pediatrics, Allergology and Gastroenterology, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun, 87-100 Toruń, PolandDepartment of Pediatrics, Allergology and Gastroenterology, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun, 87-100 Toruń, PolandThe gut microbiota in patients with food allergy, and the skin microbiota in atopic dermatitis patients differ from those of healthy people. We hypothesize that relationships may exist between gut and skin microbiota in patients with allergies. The aim of this study was to determine the possible relationship between gut and skin microbiota in patients with allergies, hence simultaneous analysis of the two compartments of microbiota was performed in infants with and without allergic symptoms. Fifty-nine infants with food allergy and/or atopic dermatitis and 28 healthy children were enrolled in the study. The skin and gut microbiota were evaluated using 16S rRNA gene amplicon sequencing. No significant differences in the α-diversity of dermal or fecal microbiota were observed between allergic and non-allergic infants; however, a significant relationship was found between bacterial community structure and allergy phenotypes, especially in the fecal samples. Certain clinical conditions were associated with characteristic bacterial taxa in the skin and gut microbiota. Positive correlations were found between skin and fecal samples in the abundance of <i>Gemella</i> among allergic infants, and <i>Lactobacillus</i> and <i>Bacteroides</i> among healthy infants. Although infants with allergies and healthy infants demonstrate microbiota with similar α-diversity, some differences in β-diversity and bacterial species abundance can be seen, which may depend on the phenotype of the allergy. For some organisms, their abundance in skin and feces samples may be correlated, and these correlations might serve as indicators of the host’s allergic state.https://www.mdpi.com/2072-6643/13/5/1682atopic dermatitisdysbiosisfood allergygutinfantsmicrobiota |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ewa Łoś-Rycharska Marcin Gołębiewski Marcin Sikora Tomasz Grzybowski Marta Gorzkiewicz Maria Popielarz Julia Gawryjołek Aneta Krogulska |
spellingShingle |
Ewa Łoś-Rycharska Marcin Gołębiewski Marcin Sikora Tomasz Grzybowski Marta Gorzkiewicz Maria Popielarz Julia Gawryjołek Aneta Krogulska A Combined Analysis of Gut and Skin Microbiota in Infants with Food Allergy and Atopic Dermatitis: A Pilot Study Nutrients atopic dermatitis dysbiosis food allergy gut infants microbiota |
author_facet |
Ewa Łoś-Rycharska Marcin Gołębiewski Marcin Sikora Tomasz Grzybowski Marta Gorzkiewicz Maria Popielarz Julia Gawryjołek Aneta Krogulska |
author_sort |
Ewa Łoś-Rycharska |
title |
A Combined Analysis of Gut and Skin Microbiota in Infants with Food Allergy and Atopic Dermatitis: A Pilot Study |
title_short |
A Combined Analysis of Gut and Skin Microbiota in Infants with Food Allergy and Atopic Dermatitis: A Pilot Study |
title_full |
A Combined Analysis of Gut and Skin Microbiota in Infants with Food Allergy and Atopic Dermatitis: A Pilot Study |
title_fullStr |
A Combined Analysis of Gut and Skin Microbiota in Infants with Food Allergy and Atopic Dermatitis: A Pilot Study |
title_full_unstemmed |
A Combined Analysis of Gut and Skin Microbiota in Infants with Food Allergy and Atopic Dermatitis: A Pilot Study |
title_sort |
combined analysis of gut and skin microbiota in infants with food allergy and atopic dermatitis: a pilot study |
publisher |
MDPI AG |
series |
Nutrients |
issn |
2072-6643 |
publishDate |
2021-05-01 |
description |
The gut microbiota in patients with food allergy, and the skin microbiota in atopic dermatitis patients differ from those of healthy people. We hypothesize that relationships may exist between gut and skin microbiota in patients with allergies. The aim of this study was to determine the possible relationship between gut and skin microbiota in patients with allergies, hence simultaneous analysis of the two compartments of microbiota was performed in infants with and without allergic symptoms. Fifty-nine infants with food allergy and/or atopic dermatitis and 28 healthy children were enrolled in the study. The skin and gut microbiota were evaluated using 16S rRNA gene amplicon sequencing. No significant differences in the α-diversity of dermal or fecal microbiota were observed between allergic and non-allergic infants; however, a significant relationship was found between bacterial community structure and allergy phenotypes, especially in the fecal samples. Certain clinical conditions were associated with characteristic bacterial taxa in the skin and gut microbiota. Positive correlations were found between skin and fecal samples in the abundance of <i>Gemella</i> among allergic infants, and <i>Lactobacillus</i> and <i>Bacteroides</i> among healthy infants. Although infants with allergies and healthy infants demonstrate microbiota with similar α-diversity, some differences in β-diversity and bacterial species abundance can be seen, which may depend on the phenotype of the allergy. For some organisms, their abundance in skin and feces samples may be correlated, and these correlations might serve as indicators of the host’s allergic state. |
topic |
atopic dermatitis dysbiosis food allergy gut infants microbiota |
url |
https://www.mdpi.com/2072-6643/13/5/1682 |
work_keys_str_mv |
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