Characterization of clarified medium from submerse and semisolid cultivation of OF Aspergillus awamori NRRL3112 by size-exclusion chromatography
In this study, a preparative size-exclusion chromatography of two different clarified media obtained from submerse and semisolid culture of the mold Aspergillus awamori was carried out. Characterization and comparison of the quantities of glucoamylase and contaminant proteins present in these media...
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Brazilian Society of Chemical Engineering
1999-01-01
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Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66321999000200012 |
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doaj-1618ce6d95004bec85a828890f6a6ea12020-11-24T22:52:30ZengBrazilian Society of Chemical EngineeringBrazilian Journal of Chemical Engineering0104-66321678-43831999-01-01162193198Characterization of clarified medium from submerse and semisolid cultivation of OF Aspergillus awamori NRRL3112 by size-exclusion chromatographyMINAMI N.M.LUCARINI A.C.KILIKIAN B.V.In this study, a preparative size-exclusion chromatography of two different clarified media obtained from submerse and semisolid culture of the mold Aspergillus awamori was carried out. Characterization and comparison of the quantities of glucoamylase and contaminant proteins present in these media were possible. Glucoamylase is the protein with the higher molecular weight in both media analyzed, varying from 72 to 80kDa in the submerse culture and from 68 to 90kDa in the semisolid culture. Also, glucoamylase protein concentration is higher in the submerse culture than in the semisolid culture. The other proteins in the submerse culture presented molecular weights lower than 12kDa and in the semisolid culture their molecular weights varied from 21 to 37kDa and below 10kDa.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66321999000200012Glucoamylasegel filtration chromatographypurificationsubmerse culturesemisolid culture |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
MINAMI N.M. LUCARINI A.C. KILIKIAN B.V. |
spellingShingle |
MINAMI N.M. LUCARINI A.C. KILIKIAN B.V. Characterization of clarified medium from submerse and semisolid cultivation of OF Aspergillus awamori NRRL3112 by size-exclusion chromatography Brazilian Journal of Chemical Engineering Glucoamylase gel filtration chromatography purification submerse culture semisolid culture |
author_facet |
MINAMI N.M. LUCARINI A.C. KILIKIAN B.V. |
author_sort |
MINAMI N.M. |
title |
Characterization of clarified medium from submerse and semisolid cultivation of OF Aspergillus awamori NRRL3112 by size-exclusion chromatography |
title_short |
Characterization of clarified medium from submerse and semisolid cultivation of OF Aspergillus awamori NRRL3112 by size-exclusion chromatography |
title_full |
Characterization of clarified medium from submerse and semisolid cultivation of OF Aspergillus awamori NRRL3112 by size-exclusion chromatography |
title_fullStr |
Characterization of clarified medium from submerse and semisolid cultivation of OF Aspergillus awamori NRRL3112 by size-exclusion chromatography |
title_full_unstemmed |
Characterization of clarified medium from submerse and semisolid cultivation of OF Aspergillus awamori NRRL3112 by size-exclusion chromatography |
title_sort |
characterization of clarified medium from submerse and semisolid cultivation of of aspergillus awamori nrrl3112 by size-exclusion chromatography |
publisher |
Brazilian Society of Chemical Engineering |
series |
Brazilian Journal of Chemical Engineering |
issn |
0104-6632 1678-4383 |
publishDate |
1999-01-01 |
description |
In this study, a preparative size-exclusion chromatography of two different clarified media obtained from submerse and semisolid culture of the mold Aspergillus awamori was carried out. Characterization and comparison of the quantities of glucoamylase and contaminant proteins present in these media were possible. Glucoamylase is the protein with the higher molecular weight in both media analyzed, varying from 72 to 80kDa in the submerse culture and from 68 to 90kDa in the semisolid culture. Also, glucoamylase protein concentration is higher in the submerse culture than in the semisolid culture. The other proteins in the submerse culture presented molecular weights lower than 12kDa and in the semisolid culture their molecular weights varied from 21 to 37kDa and below 10kDa. |
topic |
Glucoamylase gel filtration chromatography purification submerse culture semisolid culture |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66321999000200012 |
work_keys_str_mv |
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