In search for interplay between stool microRNAs, microbiota and short chain fatty acids in Crohn’s disease - a preliminary study
Abstract Background Inflammatory bowel diseases are classic polygenic disorders, with genetic loads that reflect immunopathological processes in response to the intestinal microbiota. Herein we performed the multiomics analysis by combining the large scale surveys of gut bacterial community, stool m...
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doaj-16250ea20d9f49b88ffcd9287300675b2020-11-25T03:52:14ZengBMCBMC Gastroenterology1471-230X2020-09-0120111810.1186/s12876-020-01444-3In search for interplay between stool microRNAs, microbiota and short chain fatty acids in Crohn’s disease - a preliminary studyFilip Ambrozkiewicz0Jakub Karczmarski1Maria Kulecka2Agnieszka Paziewska3Magdalena Niemira4Natalia Zeber-Lubecka5Edyta Zagorowicz6Adam Kretowski7Jerzy Ostrowski8Department of Genetics, Maria Sklodowska-Curie National Research Institute of OncologyDepartment of Genetics, Maria Sklodowska-Curie National Research Institute of OncologyDepartment of Genetics, Maria Sklodowska-Curie National Research Institute of OncologyDepartment of Genetics, Maria Sklodowska-Curie National Research Institute of OncologyClinical Research Centre, Medical University of BialystokDepartment of Gastroenterology, Hepatology and Clinical Oncology, Centre of Postgraduate Medical EducationDepartment of Gastroenterology, Hepatology and Clinical Oncology, Centre of Postgraduate Medical EducationClinical Research Centre, Medical University of BialystokDepartment of Genetics, Maria Sklodowska-Curie National Research Institute of OncologyAbstract Background Inflammatory bowel diseases are classic polygenic disorders, with genetic loads that reflect immunopathological processes in response to the intestinal microbiota. Herein we performed the multiomics analysis by combining the large scale surveys of gut bacterial community, stool microRNA (miRNA) and short chain fatty acid (SCFA) signatures to correlate their association with the activity of Crohn’s disease (CD). Methods DNA, miRNA, and metabolites were extracted from stool samples of 15 CD patients, eight with active disease and seven in remission, and nine healthy individuals. Microbial, miRNA and SCFA profiles were assessed using datasets from 16S rRNA sequencing, Nanostring miRNA and GC-MS targeted analysis, respectively. Results Pairwise comparisons showed that 9 and 23 taxa differed between controls and CD patients with active and inactive disease, respectively. Six taxa were common to both comparisons, whereas four taxa differed in CD patients. α-Diversity was lower in both CD groups than in controls. The levels of 13 miRNAs differed (p-value < 0.05; FC > 1.5) in CD patients and controls before FDR correction and 4 after. Of six SCFAs, the levels of two differed significantly (p-value < 0.05, FC > 1.5) in CD patients and controls, and the levels of four differed in patients with active and inactive CD. PLS-DA revealed models with smallest error rate for controls in bacterial component and inactive disease in metabolites. Conclusion A complex interrelationship may exist between gut dysbiosis, miRNA profiling and SCFA level in response to intestinal inflammation.http://link.springer.com/article/10.1186/s12876-020-01444-3Crohn’s disease16S rRNAmiRNASCFAsBiomarker |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Filip Ambrozkiewicz Jakub Karczmarski Maria Kulecka Agnieszka Paziewska Magdalena Niemira Natalia Zeber-Lubecka Edyta Zagorowicz Adam Kretowski Jerzy Ostrowski |
spellingShingle |
Filip Ambrozkiewicz Jakub Karczmarski Maria Kulecka Agnieszka Paziewska Magdalena Niemira Natalia Zeber-Lubecka Edyta Zagorowicz Adam Kretowski Jerzy Ostrowski In search for interplay between stool microRNAs, microbiota and short chain fatty acids in Crohn’s disease - a preliminary study BMC Gastroenterology Crohn’s disease 16S rRNA miRNA SCFAs Biomarker |
author_facet |
Filip Ambrozkiewicz Jakub Karczmarski Maria Kulecka Agnieszka Paziewska Magdalena Niemira Natalia Zeber-Lubecka Edyta Zagorowicz Adam Kretowski Jerzy Ostrowski |
author_sort |
Filip Ambrozkiewicz |
title |
In search for interplay between stool microRNAs, microbiota and short chain fatty acids in Crohn’s disease - a preliminary study |
title_short |
In search for interplay between stool microRNAs, microbiota and short chain fatty acids in Crohn’s disease - a preliminary study |
title_full |
In search for interplay between stool microRNAs, microbiota and short chain fatty acids in Crohn’s disease - a preliminary study |
title_fullStr |
In search for interplay between stool microRNAs, microbiota and short chain fatty acids in Crohn’s disease - a preliminary study |
title_full_unstemmed |
In search for interplay between stool microRNAs, microbiota and short chain fatty acids in Crohn’s disease - a preliminary study |
title_sort |
in search for interplay between stool micrornas, microbiota and short chain fatty acids in crohn’s disease - a preliminary study |
publisher |
BMC |
series |
BMC Gastroenterology |
issn |
1471-230X |
publishDate |
2020-09-01 |
description |
Abstract Background Inflammatory bowel diseases are classic polygenic disorders, with genetic loads that reflect immunopathological processes in response to the intestinal microbiota. Herein we performed the multiomics analysis by combining the large scale surveys of gut bacterial community, stool microRNA (miRNA) and short chain fatty acid (SCFA) signatures to correlate their association with the activity of Crohn’s disease (CD). Methods DNA, miRNA, and metabolites were extracted from stool samples of 15 CD patients, eight with active disease and seven in remission, and nine healthy individuals. Microbial, miRNA and SCFA profiles were assessed using datasets from 16S rRNA sequencing, Nanostring miRNA and GC-MS targeted analysis, respectively. Results Pairwise comparisons showed that 9 and 23 taxa differed between controls and CD patients with active and inactive disease, respectively. Six taxa were common to both comparisons, whereas four taxa differed in CD patients. α-Diversity was lower in both CD groups than in controls. The levels of 13 miRNAs differed (p-value < 0.05; FC > 1.5) in CD patients and controls before FDR correction and 4 after. Of six SCFAs, the levels of two differed significantly (p-value < 0.05, FC > 1.5) in CD patients and controls, and the levels of four differed in patients with active and inactive CD. PLS-DA revealed models with smallest error rate for controls in bacterial component and inactive disease in metabolites. Conclusion A complex interrelationship may exist between gut dysbiosis, miRNA profiling and SCFA level in response to intestinal inflammation. |
topic |
Crohn’s disease 16S rRNA miRNA SCFAs Biomarker |
url |
http://link.springer.com/article/10.1186/s12876-020-01444-3 |
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