Identification of single amino acid differences in uniformly charged homopolymeric peptides with aerolysin nanopore

Identification of single amino acid differences in uniformly charged homopolymeric peptides with aerolysin nanopore

Existing methods used for peptide analysis suffer from low sensitivity and specificity. Here the authors demonstrate the nanopore-based size-discrimination and purity analysis of short homopolymeric peptides with a single amino acid resolution.

Bibliographic Details
Main Authors: Fabien Piguet, Hadjer Ouldali, Manuela Pastoriza-Gallego, Philippe Manivet, Juan Pelta, Abdelghani Oukhaled
Format: Article
Language:English
Published: Nature Publishing Group 2018-03-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-018-03418-2
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spelling doaj-1633ddd585644efcac9f78479969cdde2021-05-11T09:33:25ZengNature Publishing GroupNature Communications2041-17232018-03-019111310.1038/s41467-018-03418-2Identification of single amino acid differences in uniformly charged homopolymeric peptides with aerolysin nanoporeFabien Piguet0Hadjer Ouldali1Manuela Pastoriza-Gallego2Philippe Manivet3Juan Pelta4Abdelghani Oukhaled5LAMBE UMR 8587, Université de Cergy-PontoiseLAMBE UMR 8587, Université de Cergy-PontoiseLAMBE UMR 8587, Université de Cergy-PontoiseAPHP, Centre de Ressources Biologiques BB-0033-00064, Plateforme de Bio-Pathologie et de Technologies Innovantes en santé, Hôpital LariboisièreLAMBE UMR 8587, Université d’Evry-Val-d’EssonneLAMBE UMR 8587, Université de Cergy-PontoiseExisting methods used for peptide analysis suffer from low sensitivity and specificity. Here the authors demonstrate the nanopore-based size-discrimination and purity analysis of short homopolymeric peptides with a single amino acid resolution.https://doi.org/10.1038/s41467-018-03418-2
collection DOAJ
language English
format Article
sources DOAJ
author Fabien Piguet
Hadjer Ouldali
Manuela Pastoriza-Gallego
Philippe Manivet
Juan Pelta
Abdelghani Oukhaled
spellingShingle Fabien Piguet
Hadjer Ouldali
Manuela Pastoriza-Gallego
Philippe Manivet
Juan Pelta
Abdelghani Oukhaled
Identification of single amino acid differences in uniformly charged homopolymeric peptides with aerolysin nanopore
Nature Communications
author_facet Fabien Piguet
Hadjer Ouldali
Manuela Pastoriza-Gallego
Philippe Manivet
Juan Pelta
Abdelghani Oukhaled
author_sort Fabien Piguet
title Identification of single amino acid differences in uniformly charged homopolymeric peptides with aerolysin nanopore
title_short Identification of single amino acid differences in uniformly charged homopolymeric peptides with aerolysin nanopore
title_full Identification of single amino acid differences in uniformly charged homopolymeric peptides with aerolysin nanopore
title_fullStr Identification of single amino acid differences in uniformly charged homopolymeric peptides with aerolysin nanopore
title_full_unstemmed Identification of single amino acid differences in uniformly charged homopolymeric peptides with aerolysin nanopore
title_sort identification of single amino acid differences in uniformly charged homopolymeric peptides with aerolysin nanopore
publisher Nature Publishing Group
series Nature Communications
issn 2041-1723
publishDate 2018-03-01
description Existing methods used for peptide analysis suffer from low sensitivity and specificity. Here the authors demonstrate the nanopore-based size-discrimination and purity analysis of short homopolymeric peptides with a single amino acid resolution.
url https://doi.org/10.1038/s41467-018-03418-2
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AT manuelapastorizagallego identificationofsingleaminoaciddifferencesinuniformlychargedhomopolymericpeptideswithaerolysinnanopore
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