Multicomponent Analysis of the Differential Induction of Secondary Metabolite Profiles in Fungal Endophytes
Small molecule histone deacetylase (HDAC) and DNA methyltransferase (DNMT) inhibitors are commonly used to perturb the production of fungal metabolites leading to the induction of the expression of silent biosynthetic pathways. Several reports have described the variable effects observed in natural...
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doaj-1648ccff78c44e77b3aedd494acd60f62020-11-24T21:36:22ZengMDPI AGMolecules1420-30492016-02-0121223410.3390/molecules21020234molecules21020234Multicomponent Analysis of the Differential Induction of Secondary Metabolite Profiles in Fungal EndophytesVíctor González-Menéndez0Mercedes Pérez-Bonilla1Ignacio Pérez-Victoria2Jesús Martín3Francisca Muñoz4Fernando Reyes5José R. Tormo6Olga Genilloud7Fundación MEDINA, Parque Tecnológico Ciencias de la Salud. Avda. del Conocimiento 34, 18016 Granada, SpainFundación MEDINA, Parque Tecnológico Ciencias de la Salud. Avda. del Conocimiento 34, 18016 Granada, SpainFundación MEDINA, Parque Tecnológico Ciencias de la Salud. Avda. del Conocimiento 34, 18016 Granada, SpainFundación MEDINA, Parque Tecnológico Ciencias de la Salud. Avda. del Conocimiento 34, 18016 Granada, SpainFundación MEDINA, Parque Tecnológico Ciencias de la Salud. Avda. del Conocimiento 34, 18016 Granada, SpainFundación MEDINA, Parque Tecnológico Ciencias de la Salud. Avda. del Conocimiento 34, 18016 Granada, SpainFundación MEDINA, Parque Tecnológico Ciencias de la Salud. Avda. del Conocimiento 34, 18016 Granada, SpainFundación MEDINA, Parque Tecnológico Ciencias de la Salud. Avda. del Conocimiento 34, 18016 Granada, SpainSmall molecule histone deacetylase (HDAC) and DNA methyltransferase (DNMT) inhibitors are commonly used to perturb the production of fungal metabolites leading to the induction of the expression of silent biosynthetic pathways. Several reports have described the variable effects observed in natural product profiles in fungi treated with HDAC and DNMT inhibitors, such as enhanced chemical diversity and/or the induction of new molecules previously unknown to be produced by the strain. Fungal endophytes are known to produce a wide variety of secondary metabolites (SMs) involved in their adaptation and survival within higher plants. The plant-microbe interaction may influence the expression of some biosynthetic pathways, otherwise cryptic in these fungi when grown in vitro. The aim of this study was to setup a systematic approach to evaluate and identify the possible effects of HDAC and DNMT inhibitors on the metabolic profiles of wild type fungal endophytes, including the chemical identification and characterization of the most significant SMs induced by these epigenetic modifiers.http://www.mdpi.com/1420-3049/21/2/234fungal endophytesepigenetic modifiersHDACDNMTvolcano plotssecondary metabolitesmetabolomics |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Víctor González-Menéndez Mercedes Pérez-Bonilla Ignacio Pérez-Victoria Jesús Martín Francisca Muñoz Fernando Reyes José R. Tormo Olga Genilloud |
spellingShingle |
Víctor González-Menéndez Mercedes Pérez-Bonilla Ignacio Pérez-Victoria Jesús Martín Francisca Muñoz Fernando Reyes José R. Tormo Olga Genilloud Multicomponent Analysis of the Differential Induction of Secondary Metabolite Profiles in Fungal Endophytes Molecules fungal endophytes epigenetic modifiers HDAC DNMT volcano plots secondary metabolites metabolomics |
author_facet |
Víctor González-Menéndez Mercedes Pérez-Bonilla Ignacio Pérez-Victoria Jesús Martín Francisca Muñoz Fernando Reyes José R. Tormo Olga Genilloud |
author_sort |
Víctor González-Menéndez |
title |
Multicomponent Analysis of the Differential Induction of Secondary Metabolite Profiles in Fungal Endophytes |
title_short |
Multicomponent Analysis of the Differential Induction of Secondary Metabolite Profiles in Fungal Endophytes |
title_full |
Multicomponent Analysis of the Differential Induction of Secondary Metabolite Profiles in Fungal Endophytes |
title_fullStr |
Multicomponent Analysis of the Differential Induction of Secondary Metabolite Profiles in Fungal Endophytes |
title_full_unstemmed |
Multicomponent Analysis of the Differential Induction of Secondary Metabolite Profiles in Fungal Endophytes |
title_sort |
multicomponent analysis of the differential induction of secondary metabolite profiles in fungal endophytes |
publisher |
MDPI AG |
series |
Molecules |
issn |
1420-3049 |
publishDate |
2016-02-01 |
description |
Small molecule histone deacetylase (HDAC) and DNA methyltransferase (DNMT) inhibitors are commonly used to perturb the production of fungal metabolites leading to the induction of the expression of silent biosynthetic pathways. Several reports have described the variable effects observed in natural product profiles in fungi treated with HDAC and DNMT inhibitors, such as enhanced chemical diversity and/or the induction of new molecules previously unknown to be produced by the strain. Fungal endophytes are known to produce a wide variety of secondary metabolites (SMs) involved in their adaptation and survival within higher plants. The plant-microbe interaction may influence the expression of some biosynthetic pathways, otherwise cryptic in these fungi when grown in vitro. The aim of this study was to setup a systematic approach to evaluate and identify the possible effects of HDAC and DNMT inhibitors on the metabolic profiles of wild type fungal endophytes, including the chemical identification and characterization of the most significant SMs induced by these epigenetic modifiers. |
topic |
fungal endophytes epigenetic modifiers HDAC DNMT volcano plots secondary metabolites metabolomics |
url |
http://www.mdpi.com/1420-3049/21/2/234 |
work_keys_str_mv |
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1725941361856741376 |