Efecto del carbón activado, ácido indolacético (AIA) y bencil amino purina (BAP) en el desarrollo de protocormos de Epidendrum elongatum Jacq bajo condiciones in vitro

• Main Author: Jaime Alonso Pedroza-Manrique
• Format: Article
• Language: Spanish
• Published: Universidad Nacional de Colombia 2009-06-01
• Series: Revista Colombiana de Biotecnología
• Subjects: orquídea; Epidendrum elongatum; germinación in vitro; carbón activado; AIA; BAP; Orchids; germination; in vitro; activated charcoal; IAA
• Online Access: http://www.revistas.unal.edu.co/index.php/biotecnologia/article/view/10318

<p class="MsoNormal" style="margin: 0cm 0cm 0pt; mso-layout-grid-align: none;"><span style="font-size: 10pt; color: #4d4d4d; font-family: Arial; mso-ansi-language: EN-US; mso-bidi-font-weight: bold;" lang="EN-US">The effect of activated charcoal, indol acetic acid (IAA) and benzylaminopurine (BAP) on </span><em><span style="font-size: 10pt; color: #4d4d4d; font-family: Arial; mso-ansi-language: EN-US;" lang="EN-US">Epidendrum elongatum </span></em><span style="font-size: 10pt; color: #4d4d4d; font-family: Arial; mso-ansi-language: EN-US; mso-bidi-font-weight: bold;" lang="EN-US">Jacq protocorm-like body (PLB) development </span><em><span style="font-size: 10pt; color: #4d4d4d; font-family: Arial; mso-ansi-language: EN-US;" lang="EN-US">in vitro </span></em><span style="font-size: 10pt; color: #4d4d4d; font-family: Arial; mso-ansi-language: EN-US; mso-bidi-font-weight: bold;" lang="EN-US">conditions</span><span style="font-size: 10pt; color: black; font-family: Arial; mso-ansi-language: EN-US;" lang="EN-US"></span></p><p class="MsoNormal" style="margin: 0cm 0cm 0pt; mso-layout-grid-align: none;"><strong><span style="font-size: 10pt; font-family: Arial; mso-ansi-language: EN-US;" lang="EN-US"> </span></strong></p><p class="MsoNormal" style="margin: 0cm 0cm 0pt; mso-layout-grid-align: none;"><strong><span style="font-size: 10pt; font-family: Arial;">Resumen:<span style="mso-spacerun: yes;">  </span></span></strong><span style="font-size: 10pt; font-family: Arial;">El desarrollo vegetativo de los protocormos de <em>Epidendrum elongatum </em>Jacq<em>.</em>, una orquídea endémica y en vías de extinción, se logró eficazmente mediante el cultivo <em>in vitro </em>en el medio Murashige y Skoog (1962), donde se evaluó el efecto de la interacción entre los siguientes tres factores: carbón activado (0,0; 0,5; 1,0% (w/v)); ácido indol acético (0,0; 0,5; 1,0 mg.L-1); bencil amino purina (0,0; 0,5; 1,0 mg.L-1). El medio de cultivo empleado fue enriquecido con sacarosa al 3%, y el Myo inositol al 0,1 g.L-1. En este estudio se evaluó que el efecto sobre la tasa de crecimiento de la interacción del carbón activado en concentraciones de 0,5 y 1,0% con 0,5 mg.L-1 de AIA es positivo para el desarrollo de los protocormos de <em>E. elongatum </em>bajo condiciones <em>in vitro</em>, mientras que la interacción del BAP, tanto en concentraciones de 0,5 mg.L-1 y 1,0 mg.L-1, muestra resultados no tan favorables en cuanto al grado de desarrollo de los protocormos estudiados. Este estudio describe un protocolo que permite alcanzar más de 270.000 plántulas en excelente desarrollo vegetativo dentro de 30 semanas, a partir de una cápsula de esta importante orquídea. Este protocolo es un modelo de conservación para especies que se encuentran en vías de extinción, y además permite la propagación a gran escala de <em>Epidendrum elongatum</em>.</span></p><p class="MsoNormal" style="margin: 0cm 0cm 0pt; mso-layout-grid-align: none;"><span style="font-size: 10pt; font-family: Arial;"> </span></p><p class="MsoNormal" style="margin: 0cm 0cm 0pt; mso-layout-grid-align: none;"><strong><span style="font-size: 10pt; font-family: Arial;">Palabras clave</span></strong><span style="font-size: 10pt; font-family: Arial;">: orquídea, <em>Epidendrum elongatum</em>, germinación <em>in vitro</em>, carbón activado, AIA, BAP.</span></p><p class="MsoNormal" style="margin: 0cm 0cm 0pt; mso-layout-grid-align: none;"><span style="font-size: 10pt; font-family: Arial;"> </span></p><p class="MsoNormal" style="margin: 0cm 0cm 0pt; mso-layout-grid-align: none;"><strong><span style="font-size: 10pt; font-family: Arial; mso-ansi-language: EN-US;" lang="EN-US">Abstract: </span></strong><span style="font-size: 10pt; font-family: Arial; mso-ansi-language: EN-US;" lang="EN-US">The vegetative development of protocorm-like bodies (</span><span style="font-size: 9pt; font-family: Arial; mso-ansi-language: EN-US;" lang="EN-US">PLB</span><span style="font-size: 10pt; font-family: Arial; mso-ansi-language: EN-US;" lang="EN-US">) from <em>Epidendrum elongatum </em>Jacq. (an endemic and endangered orchid) was effectively achieved through <em>in vitro </em>culture in Murashige and Skoog medium (1962); the effect of the interaction between the following three factors was studied: activated charcoal (0.0, 0.5, 1.0% (w/v)), indol acetic acid (0.0, 0.5, 1.0 mg.L</span><span style="font-size: 6pt; font-family: Arial; mso-ansi-language: EN-US;" lang="EN-US">-1</span><span style="font-size: 10pt; font-family: Arial; mso-ansi-language: EN-US;" lang="EN-US">), benzylaminopurine (0.0, 0.5, 1.0 mg.L</span><span style="font-size: 6pt; font-family: Arial; mso-ansi-language: EN-US;" lang="EN-US">-1</span><span style="font-size: 10pt; font-family: Arial; mso-ansi-language: EN-US;" lang="EN-US">). The culture medium being </span><span style="font-size: 11pt; font-family: Arial; mso-ansi-language: EN-US;" lang="EN-US">ger</span><span style="font-size: 10pt; font-family: Arial; mso-ansi-language: EN-US;" lang="EN-US">used was enriched with 3% sucrose and 0.1 g.L</span><span style="font-size: 6pt; font-family: Arial; mso-ansi-language: EN-US;" lang="EN-US">-1 </span><span style="font-size: 10pt; font-family: Arial; mso-ansi-language: EN-US;" lang="EN-US">myo-inositol. This study revealed that the effect of 0.5% and 1.0% activated charcoal concentrations interacting with 0.5 mg.L</span><span style="font-size: 6pt; font-family: Arial; mso-ansi-language: EN-US;" lang="EN-US">-1 </span><span style="font-size: 9pt; font-family: Arial; mso-ansi-language: EN-US;" lang="EN-US">AIA </span><span style="font-size: 10pt; font-family: Arial; mso-ansi-language: EN-US;" lang="EN-US">on </span><span style="font-size: 9pt; font-family: Arial; mso-ansi-language: EN-US;" lang="EN-US">PBL </span><span style="font-size: 10pt; font-family: Arial; mso-ansi-language: EN-US;" lang="EN-US">growth rate was positive for <em>E. elongatum </em></span><span style="font-size: 9pt; font-family: Arial; mso-ansi-language: EN-US;" lang="EN-US">PBL </span><span style="font-size: 10pt; font-family: Arial; mso-ansi-language: EN-US;" lang="EN-US">development <em>in vitro </em>conditions, whereas the interaction of </span><span style="font-size: 9pt; font-family: Arial; mso-ansi-language: EN-US;" lang="EN-US">BAP </span><span style="font-size: 10pt; font-family: Arial; mso-ansi-language: EN-US;" lang="EN-US">(at both 0.5 mg.L</span><span style="font-size: 6pt; font-family: Arial; mso-ansi-language: EN-US;" lang="EN-US">-1 </span><span style="font-size: 10pt; font-family: Arial; mso-ansi-language: EN-US;" lang="EN-US">and 1.0 mg.L</span><span style="font-size: 6pt; font-family: Arial; mso-ansi-language: EN-US;" lang="EN-US">-1 </span><span style="font-size: 10pt; font-family: Arial; mso-ansi-language: EN-US;" lang="EN-US">concentration) did not produce such favourable results regarding the degree of development of the </span><span style="font-size: 9pt; font-family: Arial; mso-ansi-language: EN-US;" lang="EN-US">PLB </span><span style="font-size: 10pt; font-family: Arial; mso-ansi-language: EN-US;" lang="EN-US">being studied. This study describes a protocol leading to more than 270,000 seedlings enjoying excellent vegetative growth being obtained within 30 weeks from a single capsule from this important orchid. This protocol represents a model for conserving endangered species and promotes large-scale <em>Epidendrum elongatum </em>propagation. </span></p><p class="MsoNormal" style="margin: 0cm 0cm 0pt; mso-layout-grid-align: none;"><strong><span style="font-size: 10pt; font-family: Arial; mso-ansi-language: EN-US;" lang="EN-US">Key words</span></strong><span style="font-size: 10pt; font-family: Arial; mso-ansi-language: EN-US;" lang="EN-US">: Orchids, <em>Epidendrum elongatum</em>, germination, <em>in vitro</em>, activated charcoal, </span><span style="font-size: 9pt; font-family: Arial; mso-ansi-language: EN-US;" lang="EN-US">IAA, BAP</span><span style="font-size: 10pt; font-family: Garamond; mso-bidi-font-family: Garamond; mso-ansi-language: EN-US;" lang="EN-US">.</span></p>