miR-218 overexpression suppresses tumorigenesis of papillary thyroid cancer via inactivation of PTEN /PI3K/AKT pathway by targeting Runx2
Mingkun Han,* Liwei Chen,* Yang Wang Department of Otolaryngology Head and Neck Surgery, Chinese People’s Liberation Army General Hospital, Beijing 100853, China *These author contributed equally to this work Background: It was previously reported that downregulation of miR-218 promoted...
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doaj-1672bbd386d446c8bd1707a6521487762020-11-24T21:44:39ZengDove Medical PressOncoTargets and Therapy1178-69302018-09-01Volume 116305631640987miR-218 overexpression suppresses tumorigenesis of papillary thyroid cancer via inactivation of PTEN /PI3K/AKT pathway by targeting Runx2Han MChen LWang YMingkun Han,* Liwei Chen,* Yang Wang Department of Otolaryngology Head and Neck Surgery, Chinese People’s Liberation Army General Hospital, Beijing 100853, China *These author contributed equally to this work Background: It was previously reported that downregulation of miR-218 promoted thyroid cancer cell invasion, migration, and proliferation. However, the biological functions of miR-218 and its possible regulatory mechanisms in papillary thyroid cancer (PTC) cells are still elusive. Materials and methods: The expression levels of miR-218 and Runx2 in PTC tissues and cells were determined by quantitative real-time PCR (qRT-PCR) and Western blot. The effects of miR-218 overexpression on cell viability, invasion, apoptosis, and PTEN/PI3K/AKT pathway in PTC cells were evaluated by cell counting kit-8 assay, Transwell invasion assay, flow cytometry assay, and Western blot, respectively. Luciferase reporter assay and qRT-PCR were performed to identify the target of miR-218. Xenograft tumor experiment was performed to confirm the biological roles of miR-218 and its potential mechanisms in vivo. Results: miR-218 expression was downregulated and Runx2 expression was upregulated in PTC tissues and cells. Overexpression of miR-218 suppressed viability and invasion, and induced apoptosis of PTC cells in vitro, while Runx2 overexpression greatly abolished these effects. miR-218 overexpression inactivated the PTEN/PI3K/AKT pathway, which was abated by Runx2 upregulation. Additionally, Runx2 was validated to be a direct target of miR-218. Moreover, enforced expression of miR-218 inhibited tumor growth and Runx2 expression, and blocked PTEN/PI3K/AKT pathway in vivo. Conclusion: miR-218 overexpression suppresses the tumorigenesis of PTC via downregulating PTEN/PI3K/AKT pathway by targeting Runx2, which indicates that miR-218 may be a potential therapeutic target for human PTC. Keywords: miR-218, papillary thyroid cancer, PTEN/PI3K/AKT, Runx2https://www.dovepress.com/mir-218-overexpression-suppresses-tumorigenesis-of-papillary-thyroid-c-peer-reviewed-article-OTTmiR-218tumorigenesispapillary thyroid cancerPTEN/PI3K/AKTRunx2 |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Han M Chen L Wang Y |
spellingShingle |
Han M Chen L Wang Y miR-218 overexpression suppresses tumorigenesis of papillary thyroid cancer via inactivation of PTEN /PI3K/AKT pathway by targeting Runx2 OncoTargets and Therapy miR-218 tumorigenesis papillary thyroid cancer PTEN/PI3K/AKT Runx2 |
author_facet |
Han M Chen L Wang Y |
author_sort |
Han M |
title |
miR-218 overexpression suppresses tumorigenesis of papillary thyroid cancer via inactivation of PTEN /PI3K/AKT pathway by targeting Runx2 |
title_short |
miR-218 overexpression suppresses tumorigenesis of papillary thyroid cancer via inactivation of PTEN /PI3K/AKT pathway by targeting Runx2 |
title_full |
miR-218 overexpression suppresses tumorigenesis of papillary thyroid cancer via inactivation of PTEN /PI3K/AKT pathway by targeting Runx2 |
title_fullStr |
miR-218 overexpression suppresses tumorigenesis of papillary thyroid cancer via inactivation of PTEN /PI3K/AKT pathway by targeting Runx2 |
title_full_unstemmed |
miR-218 overexpression suppresses tumorigenesis of papillary thyroid cancer via inactivation of PTEN /PI3K/AKT pathway by targeting Runx2 |
title_sort |
mir-218 overexpression suppresses tumorigenesis of papillary thyroid cancer via inactivation of pten /pi3k/akt pathway by targeting runx2 |
publisher |
Dove Medical Press |
series |
OncoTargets and Therapy |
issn |
1178-6930 |
publishDate |
2018-09-01 |
description |
Mingkun Han,* Liwei Chen,* Yang Wang Department of Otolaryngology Head and Neck Surgery, Chinese People’s Liberation Army General Hospital, Beijing 100853, China *These author contributed equally to this work Background: It was previously reported that downregulation of miR-218 promoted thyroid cancer cell invasion, migration, and proliferation. However, the biological functions of miR-218 and its possible regulatory mechanisms in papillary thyroid cancer (PTC) cells are still elusive. Materials and methods: The expression levels of miR-218 and Runx2 in PTC tissues and cells were determined by quantitative real-time PCR (qRT-PCR) and Western blot. The effects of miR-218 overexpression on cell viability, invasion, apoptosis, and PTEN/PI3K/AKT pathway in PTC cells were evaluated by cell counting kit-8 assay, Transwell invasion assay, flow cytometry assay, and Western blot, respectively. Luciferase reporter assay and qRT-PCR were performed to identify the target of miR-218. Xenograft tumor experiment was performed to confirm the biological roles of miR-218 and its potential mechanisms in vivo. Results: miR-218 expression was downregulated and Runx2 expression was upregulated in PTC tissues and cells. Overexpression of miR-218 suppressed viability and invasion, and induced apoptosis of PTC cells in vitro, while Runx2 overexpression greatly abolished these effects. miR-218 overexpression inactivated the PTEN/PI3K/AKT pathway, which was abated by Runx2 upregulation. Additionally, Runx2 was validated to be a direct target of miR-218. Moreover, enforced expression of miR-218 inhibited tumor growth and Runx2 expression, and blocked PTEN/PI3K/AKT pathway in vivo. Conclusion: miR-218 overexpression suppresses the tumorigenesis of PTC via downregulating PTEN/PI3K/AKT pathway by targeting Runx2, which indicates that miR-218 may be a potential therapeutic target for human PTC. Keywords: miR-218, papillary thyroid cancer, PTEN/PI3K/AKT, Runx2 |
topic |
miR-218 tumorigenesis papillary thyroid cancer PTEN/PI3K/AKT Runx2 |
url |
https://www.dovepress.com/mir-218-overexpression-suppresses-tumorigenesis-of-papillary-thyroid-c-peer-reviewed-article-OTT |
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