Role of Yanghe Huayan Decoction in ER-PI3K/Akt/mTOR pathway of endocrine-resistant breast cancer cells with low mTOR expression

• Main Authors: Xue Sun, Jing-Wei Li
• Format: Article
• Language: English
• Published: Editorial Board of Journal of Hainan Medical University 2020-07-01
• Series: Journal of Hainan Medical University
• Subjects: yanghe huayan decoction; breast cancer; endocrine resistance; low-level expression of mtor; er-pi3k/akt/mtor
• Online Access: http://www.hnykdxxb.com/PDF/202014/06.pdf

Objective: To study the mechanism of action of Yanghe Huayan Decoction on endocrineresistant cells (MCF-7R/mTOR cells) with low expression of mTOR; Methods: CCK-8 assay and cell clone assay were used to detect cell proliferation and clonal ability, and flow cytometry was used to detect cell apoptosis.The changes of cytokines in ER-PI3K/Akt/mTOR signaling pathway were analyzed by blot and QPCR. Results: Yanghe Huayan Decoction could significantly affect the proliferation (p < 0.05) and cloning ability (p < 0.05) of MCF-7R/ mTOR cells, promote cell apoptosis (p < 0.01), and downregulate the expression of ER, PI3K, AKT, mTOR and p-mTOR (p < 0.05).Conclusion: Yanghe Huayan Decoction can regulate the ER-PI3K/Akt/mTOR signaling pathway with multiple targets, and the use of combined mTOR inhibitors can regulate the ER-PI3K/Akt/mTOR signaling pathway more significantly.