GFAP antibodies show protective effect on oxidatively stressed neuroretinal cells via interaction with ERP57

The pathogenesis of glaucoma, a common neurodegenerative disease, involves an immunologic component. Changes in the natural autoantibody profile of glaucoma patients were detected, showing not only up-regulated but also down-regulated immunoreactivities. In recent studies we were able to demonstrate...

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Main Authors: C. Wilding, K. Bell, S. Funke, S. Beck, Norbert Pfeiffer, Franz H. Grus
Format: Article
Language:English
Published: Elsevier 2015-03-01
Series:Journal of Pharmacological Sciences
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S1347861315000389
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spelling doaj-168abe58b368403da92025aff25b32e12020-11-24T22:59:43ZengElsevierJournal of Pharmacological Sciences1347-86132015-03-01127329830410.1016/j.jphs.2014.12.019GFAP antibodies show protective effect on oxidatively stressed neuroretinal cells via interaction with ERP57C. WildingK. BellS. FunkeS. BeckNorbert PfeifferFranz H. GrusThe pathogenesis of glaucoma, a common neurodegenerative disease, involves an immunologic component. Changes in the natural autoantibody profile of glaucoma patients were detected, showing not only up-regulated but also down-regulated immunoreactivities. In recent studies we were able to demonstrate that the antibody changes have a large influence on protein profiles of neuroretinal cells. Furthermore we could demonstrate neuroprotective potential of one of the down-regulated antibodies (γ-synuclein antibody). Anti-GFAP antibody is another antibody found down-regulated in glaucoma patients. Since GFAP expression is intensified in glaucomatous retina, the aim of this study was to detect the effect of GFAP antibodies on neuroretinal cells. This is realized with a viability-test as well as proteomic analysis of cells incubated with GFAP antibodies. Furthermore, possible interaction partners of the GFAP antibody in neuroretinal cells were identified by western blot, mass spectrometry and indirect immunofluorescence staining. We found that the GFAP antibody is able to protect cells from oxidative stress, which is due to changed protein expressions of the actin cytoskeleton. Furthermore we detected a cross-reaction of the antibody to endoplasmic reticulum resident protein 57 on the cell membrane, which seems to lead to a changed signaling in the cells triggering the protective effects.http://www.sciencedirect.com/science/article/pii/S1347861315000389NeuroprotectionGlaucomaAutoimmunityGFAPERP57
collection DOAJ
language English
format Article
sources DOAJ
author C. Wilding
K. Bell
S. Funke
S. Beck
Norbert Pfeiffer
Franz H. Grus
spellingShingle C. Wilding
K. Bell
S. Funke
S. Beck
Norbert Pfeiffer
Franz H. Grus
GFAP antibodies show protective effect on oxidatively stressed neuroretinal cells via interaction with ERP57
Journal of Pharmacological Sciences
Neuroprotection
Glaucoma
Autoimmunity
GFAP
ERP57
author_facet C. Wilding
K. Bell
S. Funke
S. Beck
Norbert Pfeiffer
Franz H. Grus
author_sort C. Wilding
title GFAP antibodies show protective effect on oxidatively stressed neuroretinal cells via interaction with ERP57
title_short GFAP antibodies show protective effect on oxidatively stressed neuroretinal cells via interaction with ERP57
title_full GFAP antibodies show protective effect on oxidatively stressed neuroretinal cells via interaction with ERP57
title_fullStr GFAP antibodies show protective effect on oxidatively stressed neuroretinal cells via interaction with ERP57
title_full_unstemmed GFAP antibodies show protective effect on oxidatively stressed neuroretinal cells via interaction with ERP57
title_sort gfap antibodies show protective effect on oxidatively stressed neuroretinal cells via interaction with erp57
publisher Elsevier
series Journal of Pharmacological Sciences
issn 1347-8613
publishDate 2015-03-01
description The pathogenesis of glaucoma, a common neurodegenerative disease, involves an immunologic component. Changes in the natural autoantibody profile of glaucoma patients were detected, showing not only up-regulated but also down-regulated immunoreactivities. In recent studies we were able to demonstrate that the antibody changes have a large influence on protein profiles of neuroretinal cells. Furthermore we could demonstrate neuroprotective potential of one of the down-regulated antibodies (γ-synuclein antibody). Anti-GFAP antibody is another antibody found down-regulated in glaucoma patients. Since GFAP expression is intensified in glaucomatous retina, the aim of this study was to detect the effect of GFAP antibodies on neuroretinal cells. This is realized with a viability-test as well as proteomic analysis of cells incubated with GFAP antibodies. Furthermore, possible interaction partners of the GFAP antibody in neuroretinal cells were identified by western blot, mass spectrometry and indirect immunofluorescence staining. We found that the GFAP antibody is able to protect cells from oxidative stress, which is due to changed protein expressions of the actin cytoskeleton. Furthermore we detected a cross-reaction of the antibody to endoplasmic reticulum resident protein 57 on the cell membrane, which seems to lead to a changed signaling in the cells triggering the protective effects.
topic Neuroprotection
Glaucoma
Autoimmunity
GFAP
ERP57
url http://www.sciencedirect.com/science/article/pii/S1347861315000389
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