The Development of Pathogenicity of Avian Influenza Virus Isolated from Indonesia

• Main Authors: Michael Haryadi Wibowo, Agus Eko Srihanto, Khrisdiana Putri, Widya Asmara, Charles Rangga Tabbu
• Format: Article
• Language: English
• Published: Universitas Gadjah Mada, Yogyakarta 2015-11-01
• Series: Indonesian Journal of Biotechnology
• Online Access: http://journal.ugm.ac.id/ijbiotech/article/view/7876

<div>Highly pathogenic avian infl uenza outbreak in Indonesia has been reported in various poultry due to</div><div>H5N1 subtype. The presence of multiple basic amino acids within the cleavage site of HA glycoprotein has</div><div>been identifi ed to be associated with the pathogenicity of avian infl uenza virus. The study was retrospective</div><div>study which was designed to characterize the cleavage site and fusion site region of haemagglutinin gene of</div><div>AIV isolated from various poultry in 2003 to 2013. Isolation, Identifi cation and propagation were carried out</div><div>to collect viral stock. For virus detection, reverse transcriptase PCR (RT-PCR) method on H5 and N1 gene</div><div>fragment was performed. All of RT-PCR HA gene positive products were sequenced for further nucleotide</div><div>analysis and to determine the nucleotide composition at the targeted fragment. The results are all AIV isolates</div><div>were identifi ed as H5N1 subtype. The sequence analyses revealed some motives of basic amino acid motive</div><div>that were classifi ed as highly pathogenic avian infl uenza virus. Further analyses on fusion domain of all AIV</div><div>isolated during the period 2003 to 2013 showed conserved amino acid.</div><div><br /></div><div>Keywords: avian infl uenza, haemagglutinin, cleavage site, basic amino acid, fusion site</div>