Transcriptional and Metabolic Profiling of Potato Plants Expressing a Plastid-Targeted Electron Shuttle Reveal Modulation of Genes Associated to Drought Tolerance by Chloroplast Redox Poise
Water limitation represents the main environmental constraint affecting crop yield worldwide. Photosynthesis is a primary drought target, resulting in over-reduction of the photosynthetic electron transport chain and increased production of reactive oxygen species in plastids. Manipulation of chloro...
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doaj-169b56b28f0d4e97b531b84e3ff920f22020-11-25T03:28:26ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-09-01217199719910.3390/ijms21197199Transcriptional and Metabolic Profiling of Potato Plants Expressing a Plastid-Targeted Electron Shuttle Reveal Modulation of Genes Associated to Drought Tolerance by Chloroplast Redox PoiseJuan J. Pierella Karlusich0Rocío C. Arce1Fahimeh Shahinnia2Sophia Sonnewald3Uwe Sonnewald4Matias D. Zurbriggen5Mohammad-Reza Hajirezaei6Néstor Carrillo7Instituto de Biología Molecular y Celular de Rosario (IBR-UNR/CONICET), Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario (UNR), Rosario 2000, ArgentinaInstituto de Biología Molecular y Celular de Rosario (IBR-UNR/CONICET), Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario (UNR), Rosario 2000, ArgentinaLeibniz Institute of Plant Genetics and Crop Plant Research, OT Gatersleben, Corrensstrasse, D-06466 Stadt Seeland, GermanyDivision of Biochemistry, Department of Biology, Friedrich-Alexander-University Erlangen-Nurenberg, 91058 Erlangen, GermanyDivision of Biochemistry, Department of Biology, Friedrich-Alexander-University Erlangen-Nurenberg, 91058 Erlangen, GermanyInstitute of Synthetic Biology and CEPLAS, University of Düsseldorf, Universitätsstr, 1 40225 Düsseldorf, GermanyLeibniz Institute of Plant Genetics and Crop Plant Research, OT Gatersleben, Corrensstrasse, D-06466 Stadt Seeland, GermanyInstituto de Biología Molecular y Celular de Rosario (IBR-UNR/CONICET), Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario (UNR), Rosario 2000, ArgentinaWater limitation represents the main environmental constraint affecting crop yield worldwide. Photosynthesis is a primary drought target, resulting in over-reduction of the photosynthetic electron transport chain and increased production of reactive oxygen species in plastids. Manipulation of chloroplast electron distribution by introducing alternative electron transport sinks has been shown to increase plant tolerance to multiple environmental challenges including hydric stress, suggesting that a similar strategy could be used to improve drought tolerance in crops. We show herein that the expression of the cyanobacterial electron shuttle flavodoxin in potato chloroplasts protected photosynthetic activities even at a pre-symptomatic stage of drought. Transcriptional and metabolic profiling revealed an attenuated response to the adverse condition in flavodoxin-expressing plants, correlating with their increased stress tolerance. Interestingly, 5–6% of leaf-expressed genes were affected by flavodoxin in the absence of drought, representing pathways modulated by chloroplast redox status during normal growth. About 300 of these genes potentially contribute to stress acclimation as their modulation by flavodoxin proceeds in the same direction as their drought response in wild-type plants. Tuber yield losses under chronic water limitation were mitigated in flavodoxin-expressing plants, indicating that the flavoprotein has the potential to improve major agronomic traits in potato.https://www.mdpi.com/1422-0067/21/19/7199droughtstress responsesphotosynthesischloroplast redox statusflavodoxintranscriptomics |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Juan J. Pierella Karlusich Rocío C. Arce Fahimeh Shahinnia Sophia Sonnewald Uwe Sonnewald Matias D. Zurbriggen Mohammad-Reza Hajirezaei Néstor Carrillo |
spellingShingle |
Juan J. Pierella Karlusich Rocío C. Arce Fahimeh Shahinnia Sophia Sonnewald Uwe Sonnewald Matias D. Zurbriggen Mohammad-Reza Hajirezaei Néstor Carrillo Transcriptional and Metabolic Profiling of Potato Plants Expressing a Plastid-Targeted Electron Shuttle Reveal Modulation of Genes Associated to Drought Tolerance by Chloroplast Redox Poise International Journal of Molecular Sciences drought stress responses photosynthesis chloroplast redox status flavodoxin transcriptomics |
author_facet |
Juan J. Pierella Karlusich Rocío C. Arce Fahimeh Shahinnia Sophia Sonnewald Uwe Sonnewald Matias D. Zurbriggen Mohammad-Reza Hajirezaei Néstor Carrillo |
author_sort |
Juan J. Pierella Karlusich |
title |
Transcriptional and Metabolic Profiling of Potato Plants Expressing a Plastid-Targeted Electron Shuttle Reveal Modulation of Genes Associated to Drought Tolerance by Chloroplast Redox Poise |
title_short |
Transcriptional and Metabolic Profiling of Potato Plants Expressing a Plastid-Targeted Electron Shuttle Reveal Modulation of Genes Associated to Drought Tolerance by Chloroplast Redox Poise |
title_full |
Transcriptional and Metabolic Profiling of Potato Plants Expressing a Plastid-Targeted Electron Shuttle Reveal Modulation of Genes Associated to Drought Tolerance by Chloroplast Redox Poise |
title_fullStr |
Transcriptional and Metabolic Profiling of Potato Plants Expressing a Plastid-Targeted Electron Shuttle Reveal Modulation of Genes Associated to Drought Tolerance by Chloroplast Redox Poise |
title_full_unstemmed |
Transcriptional and Metabolic Profiling of Potato Plants Expressing a Plastid-Targeted Electron Shuttle Reveal Modulation of Genes Associated to Drought Tolerance by Chloroplast Redox Poise |
title_sort |
transcriptional and metabolic profiling of potato plants expressing a plastid-targeted electron shuttle reveal modulation of genes associated to drought tolerance by chloroplast redox poise |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1661-6596 1422-0067 |
publishDate |
2020-09-01 |
description |
Water limitation represents the main environmental constraint affecting crop yield worldwide. Photosynthesis is a primary drought target, resulting in over-reduction of the photosynthetic electron transport chain and increased production of reactive oxygen species in plastids. Manipulation of chloroplast electron distribution by introducing alternative electron transport sinks has been shown to increase plant tolerance to multiple environmental challenges including hydric stress, suggesting that a similar strategy could be used to improve drought tolerance in crops. We show herein that the expression of the cyanobacterial electron shuttle flavodoxin in potato chloroplasts protected photosynthetic activities even at a pre-symptomatic stage of drought. Transcriptional and metabolic profiling revealed an attenuated response to the adverse condition in flavodoxin-expressing plants, correlating with their increased stress tolerance. Interestingly, 5–6% of leaf-expressed genes were affected by flavodoxin in the absence of drought, representing pathways modulated by chloroplast redox status during normal growth. About 300 of these genes potentially contribute to stress acclimation as their modulation by flavodoxin proceeds in the same direction as their drought response in wild-type plants. Tuber yield losses under chronic water limitation were mitigated in flavodoxin-expressing plants, indicating that the flavoprotein has the potential to improve major agronomic traits in potato. |
topic |
drought stress responses photosynthesis chloroplast redox status flavodoxin transcriptomics |
url |
https://www.mdpi.com/1422-0067/21/19/7199 |
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