Green synthesis of silver nanoparticles using the seaweed Gracilaria birdiae and their antibacterial activity

This work presents a simple method for the green synthesis of silver nanoparticles (AgNPs) using as reducing and stabilizing agent a polysaccharide extracted from red algae Gracilaria birdiae present in the coast of Piauí. The AgNPS were prepared using three polysaccharide concentrations (0.02, 0.03...

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Main Authors:	Anderson Passos de Aragão, Taiane Maria de Oliveira, Patrick Veras Quelemes, Márcia Luana Gomes Perfeito, Maria Carvalho Araújo, Janaína de Araújo Sousa Santiago, Vinicius S. Cardoso, Pedro Quaresma, José Roberto de Souza de Almeida Leite, Durcilene Alves da Silva
Format:	Article
Language:	English
Published:	Elsevier 2019-12-01
Series:	Arabian Journal of Chemistry
Online Access:	http://www.sciencedirect.com/science/article/pii/S1878535216300387

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record_format	Article
spelling	doaj-169cca08e1e6490f9fbd792a01ef28582020-11-25T02:09:36ZengElsevierArabian Journal of Chemistry1878-53522019-12-0112841824188Green synthesis of silver nanoparticles using the seaweed Gracilaria birdiae and their antibacterial activityAnderson Passos de Aragão0Taiane Maria de Oliveira1Patrick Veras Quelemes2Márcia Luana Gomes Perfeito3Maria Carvalho Araújo4Janaína de Araújo Sousa Santiago5Vinicius S. Cardoso6Pedro Quaresma7José Roberto de Souza de Almeida Leite8Durcilene Alves da Silva9Núcleo de Pesquisa em Biodiversidade e Biotecnologia, BIOTEC, Campus Ministro Reis Veloso, Universidade Federal do Piauí, UFPI, 64202020 Parnaíba, PI, BrazilNúcleo de Pesquisa em Biodiversidade e Biotecnologia, BIOTEC, Campus Ministro Reis Veloso, Universidade Federal do Piauí, UFPI, 64202020 Parnaíba, PI, BrazilNúcleo de Pesquisa em Biodiversidade e Biotecnologia, BIOTEC, Campus Ministro Reis Veloso, Universidade Federal do Piauí, UFPI, 64202020 Parnaíba, PI, BrazilNúcleo de Pesquisa em Biodiversidade e Biotecnologia, BIOTEC, Campus Ministro Reis Veloso, Universidade Federal do Piauí, UFPI, 64202020 Parnaíba, PI, BrazilNúcleo de Pesquisa em Biodiversidade e Biotecnologia, BIOTEC, Campus Ministro Reis Veloso, Universidade Federal do Piauí, UFPI, 64202020 Parnaíba, PI, BrazilNúcleo de Pesquisa em Biodiversidade e Biotecnologia, BIOTEC, Campus Ministro Reis Veloso, Universidade Federal do Piauí, UFPI, 64202020 Parnaíba, PI, BrazilNúcleo de Pesquisa em Biodiversidade e Biotecnologia, BIOTEC, Campus Ministro Reis Veloso, Universidade Federal do Piauí, UFPI, 64202020 Parnaíba, PI, BrazilREQUIMTE-UCIBIO, Departamento de Química e Bioquímica, Faculdade de Ciências, Universidade do Porto, 4169-007 Porto, PortugalNúcleo de Pesquisa em Biodiversidade e Biotecnologia, BIOTEC, Campus Ministro Reis Veloso, Universidade Federal do Piauí, UFPI, 64202020 Parnaíba, PI, BrazilNúcleo de Pesquisa em Biodiversidade e Biotecnologia, BIOTEC, Campus Ministro Reis Veloso, Universidade Federal do Piauí, UFPI, 64202020 Parnaíba, PI, Brazil; Corresponding author at: Center for Biodiversity Research and Biotechnology (BIOTEC), Federal University of Piauí, Campus Minister Reis Velloso, Parnaíba, Piauí Avenida São Sebastião CEP 64202020, Brazil. Tel.: +55 86 33235433.This work presents a simple method for the green synthesis of silver nanoparticles (AgNPs) using as reducing and stabilizing agent a polysaccharide extracted from red algae Gracilaria birdiae present in the coast of Piauí. The AgNPS were prepared using three polysaccharide concentrations (0.02, 0.03 and 0.05% v/v) and two pHs (10 and 11) at stirring for 30 min at 90 °C. The formation of silver nanoparticles was monitored by measurements of UV–vis and FTIR and characterized by size and zeta potential measurements using DLS and morphologically by TEM. The UV–vis absorption spectrum showed the surface plasmon peak at 410 nm, which is characteristic peak of silver nanoparticles. The functional biomolecules present in the polysaccharide and the interaction between the nanoparticles were identified by the Fourier transform infrared spectroscopy (FTIR) analysis. The stability of the synthesized silver nanoparticles was analyzed during four months and no significant agglomeration was observed. The hydrodynamic diameter of the AgNPs varied between 20.2 nm and 94.9 nm. The AgNPs were tested for antimicrobial activity using Escherichia coli (Gram-negative) and Staphylococcus aureus (Gram-positive) and all samples showed antimicrobial activity against E. coli. Using an environment-friendly, the AgNPs were synthesized in a simple, rapid and one-step process using natural sources as red algae with favorable characteristics such as spherical shape, small size and zeta potential negative. The results suggest that the polysaccharide mediated synthesized silver nanoparticles could be used as a model for future projects of nano-medicines or drug delivery systems. Keywords: Silver nanoparticles, Green synthesis, Algae, Gracilaria birdiaehttp://www.sciencedirect.com/science/article/pii/S1878535216300387
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Anderson Passos de Aragão Taiane Maria de Oliveira Patrick Veras Quelemes Márcia Luana Gomes Perfeito Maria Carvalho Araújo Janaína de Araújo Sousa Santiago Vinicius S. Cardoso Pedro Quaresma José Roberto de Souza de Almeida Leite Durcilene Alves da Silva
spellingShingle	Anderson Passos de Aragão Taiane Maria de Oliveira Patrick Veras Quelemes Márcia Luana Gomes Perfeito Maria Carvalho Araújo Janaína de Araújo Sousa Santiago Vinicius S. Cardoso Pedro Quaresma José Roberto de Souza de Almeida Leite Durcilene Alves da Silva Green synthesis of silver nanoparticles using the seaweed Gracilaria birdiae and their antibacterial activity Arabian Journal of Chemistry
author_facet	Anderson Passos de Aragão Taiane Maria de Oliveira Patrick Veras Quelemes Márcia Luana Gomes Perfeito Maria Carvalho Araújo Janaína de Araújo Sousa Santiago Vinicius S. Cardoso Pedro Quaresma José Roberto de Souza de Almeida Leite Durcilene Alves da Silva
author_sort	Anderson Passos de Aragão
title	Green synthesis of silver nanoparticles using the seaweed Gracilaria birdiae and their antibacterial activity
title_short	Green synthesis of silver nanoparticles using the seaweed Gracilaria birdiae and their antibacterial activity
title_full	Green synthesis of silver nanoparticles using the seaweed Gracilaria birdiae and their antibacterial activity
title_fullStr	Green synthesis of silver nanoparticles using the seaweed Gracilaria birdiae and their antibacterial activity
title_full_unstemmed	Green synthesis of silver nanoparticles using the seaweed Gracilaria birdiae and their antibacterial activity
title_sort	green synthesis of silver nanoparticles using the seaweed gracilaria birdiae and their antibacterial activity
publisher	Elsevier
series	Arabian Journal of Chemistry
issn	1878-5352
publishDate	2019-12-01
description	This work presents a simple method for the green synthesis of silver nanoparticles (AgNPs) using as reducing and stabilizing agent a polysaccharide extracted from red algae Gracilaria birdiae present in the coast of Piauí. The AgNPS were prepared using three polysaccharide concentrations (0.02, 0.03 and 0.05% v/v) and two pHs (10 and 11) at stirring for 30 min at 90 °C. The formation of silver nanoparticles was monitored by measurements of UV–vis and FTIR and characterized by size and zeta potential measurements using DLS and morphologically by TEM. The UV–vis absorption spectrum showed the surface plasmon peak at 410 nm, which is characteristic peak of silver nanoparticles. The functional biomolecules present in the polysaccharide and the interaction between the nanoparticles were identified by the Fourier transform infrared spectroscopy (FTIR) analysis. The stability of the synthesized silver nanoparticles was analyzed during four months and no significant agglomeration was observed. The hydrodynamic diameter of the AgNPs varied between 20.2 nm and 94.9 nm. The AgNPs were tested for antimicrobial activity using Escherichia coli (Gram-negative) and Staphylococcus aureus (Gram-positive) and all samples showed antimicrobial activity against E. coli. Using an environment-friendly, the AgNPs were synthesized in a simple, rapid and one-step process using natural sources as red algae with favorable characteristics such as spherical shape, small size and zeta potential negative. The results suggest that the polysaccharide mediated synthesized silver nanoparticles could be used as a model for future projects of nano-medicines or drug delivery systems. Keywords: Silver nanoparticles, Green synthesis, Algae, Gracilaria birdiae
url	http://www.sciencedirect.com/science/article/pii/S1878535216300387
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Green synthesis of silver nanoparticles using the seaweed Gracilaria birdiae and their antibacterial activity

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