Chrysolina herbacea modulates terpenoid biosynthesis of Mentha aquatica L.
Interactions between herbivorous insects and plants storing terpenoids are poorly understood. This study describes the ability of Chrysolina herbacea to use volatiles emitted by undamaged Mentha aquatica plants as attractants and the plant's response to herbivory, which involves the production...
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2011-01-01
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doaj-16a04a7571e042159090410cf82f72ee2020-11-25T02:09:18ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-0163e1719510.1371/journal.pone.0017195Chrysolina herbacea modulates terpenoid biosynthesis of Mentha aquatica L.Simon Atsbaha ZebeloCinzia M BerteaSimone BossiAndrea OcchipintiGiorgio GnaviMassimo E MaffeiInteractions between herbivorous insects and plants storing terpenoids are poorly understood. This study describes the ability of Chrysolina herbacea to use volatiles emitted by undamaged Mentha aquatica plants as attractants and the plant's response to herbivory, which involves the production of deterrent molecules. Emitted plant volatiles were analyzed by GC-MS. The insect's response to plant volatiles was tested by Y-tube olfactometer bioassays. Total RNA was extracted from control plants, mechanically damaged leaves, and leaves damaged by herbivores. The terpenoid quantitative gene expressions (qPCR) were then assayed. Upon herbivory, M. aquatica synthesizes and emits (+)-menthofuran, which acts as a deterrent to C. herbacea. Herbivory was found to up-regulate the expression of genes involved in terpenoid biosynthesis. The increased emission of (+)-menthofuran was correlated with the upregulation of (+)-menthofuran synthase.http://europepmc.org/articles/PMC3052309?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Simon Atsbaha Zebelo Cinzia M Bertea Simone Bossi Andrea Occhipinti Giorgio Gnavi Massimo E Maffei |
spellingShingle |
Simon Atsbaha Zebelo Cinzia M Bertea Simone Bossi Andrea Occhipinti Giorgio Gnavi Massimo E Maffei Chrysolina herbacea modulates terpenoid biosynthesis of Mentha aquatica L. PLoS ONE |
author_facet |
Simon Atsbaha Zebelo Cinzia M Bertea Simone Bossi Andrea Occhipinti Giorgio Gnavi Massimo E Maffei |
author_sort |
Simon Atsbaha Zebelo |
title |
Chrysolina herbacea modulates terpenoid biosynthesis of Mentha aquatica L. |
title_short |
Chrysolina herbacea modulates terpenoid biosynthesis of Mentha aquatica L. |
title_full |
Chrysolina herbacea modulates terpenoid biosynthesis of Mentha aquatica L. |
title_fullStr |
Chrysolina herbacea modulates terpenoid biosynthesis of Mentha aquatica L. |
title_full_unstemmed |
Chrysolina herbacea modulates terpenoid biosynthesis of Mentha aquatica L. |
title_sort |
chrysolina herbacea modulates terpenoid biosynthesis of mentha aquatica l. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2011-01-01 |
description |
Interactions between herbivorous insects and plants storing terpenoids are poorly understood. This study describes the ability of Chrysolina herbacea to use volatiles emitted by undamaged Mentha aquatica plants as attractants and the plant's response to herbivory, which involves the production of deterrent molecules. Emitted plant volatiles were analyzed by GC-MS. The insect's response to plant volatiles was tested by Y-tube olfactometer bioassays. Total RNA was extracted from control plants, mechanically damaged leaves, and leaves damaged by herbivores. The terpenoid quantitative gene expressions (qPCR) were then assayed. Upon herbivory, M. aquatica synthesizes and emits (+)-menthofuran, which acts as a deterrent to C. herbacea. Herbivory was found to up-regulate the expression of genes involved in terpenoid biosynthesis. The increased emission of (+)-menthofuran was correlated with the upregulation of (+)-menthofuran synthase. |
url |
http://europepmc.org/articles/PMC3052309?pdf=render |
work_keys_str_mv |
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