MALARIA DIAGNOSIS BY LOOP-MEDIATED ISOTHERMAL AMPLIFICATION (LAMP) IN THAILAND
The loop-mediated isothermal amplification method (LAMP) is a recently developed molecular technique that amplifies nucleic acid under isothermal conditions. For malaria diagnosis, 150 blood samples from consecutive febrile malaria patients, and healthy subjects were screened in Thailand. Each sampl...
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doaj-16c003af268a40b082360d3b3c3f5c1b2020-11-25T02:42:29ZengUniversidade de São PauloRevista do Instituto de Medicina Tropical de São Paulo1678-99462016-01-0158010.1590/S1678-9946201658027S0036-46652016005000220MALARIA DIAGNOSIS BY LOOP-MEDIATED ISOTHERMAL AMPLIFICATION (LAMP) IN THAILANDRonja OCKERYongyut PROMPUNJAISalakchit CHUTIPONGVIVATEPanagiotis KARANISThe loop-mediated isothermal amplification method (LAMP) is a recently developed molecular technique that amplifies nucleic acid under isothermal conditions. For malaria diagnosis, 150 blood samples from consecutive febrile malaria patients, and healthy subjects were screened in Thailand. Each sample was diagnosed by LAMP, microscopy and nested polymerase chain reaction (nPCR), using nPCR as the gold standard. Malaria LAMP was performed using Plasmodiumgenus and Plasmodium falciparum specific assays in parallel. For the genus Plasmodium, microscopy showed a sensitivity and specificity of 100%, while LAMP presented 99% of sensitivity and 93% of specificity. For P. falciparum, microscopy had a sensitivity of 95%, and LAMP of 90%, regarding the specificity; and microscopy presented 93% and LAMP 97% of specificity. The results of the genus-specific LAMP technique were highly consistent with those of nPCR and the sensitivity of P. falciparum detection was only marginally lower.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652016005000220&lng=en&tlng=enMalariaPlasmodiumMolecular DiagnosisLAMPThailand |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ronja OCKER Yongyut PROMPUNJAI Salakchit CHUTIPONGVIVATE Panagiotis KARANIS |
spellingShingle |
Ronja OCKER Yongyut PROMPUNJAI Salakchit CHUTIPONGVIVATE Panagiotis KARANIS MALARIA DIAGNOSIS BY LOOP-MEDIATED ISOTHERMAL AMPLIFICATION (LAMP) IN THAILAND Revista do Instituto de Medicina Tropical de São Paulo Malaria Plasmodium Molecular Diagnosis LAMP Thailand |
author_facet |
Ronja OCKER Yongyut PROMPUNJAI Salakchit CHUTIPONGVIVATE Panagiotis KARANIS |
author_sort |
Ronja OCKER |
title |
MALARIA DIAGNOSIS BY LOOP-MEDIATED ISOTHERMAL AMPLIFICATION (LAMP) IN THAILAND |
title_short |
MALARIA DIAGNOSIS BY LOOP-MEDIATED ISOTHERMAL AMPLIFICATION (LAMP) IN THAILAND |
title_full |
MALARIA DIAGNOSIS BY LOOP-MEDIATED ISOTHERMAL AMPLIFICATION (LAMP) IN THAILAND |
title_fullStr |
MALARIA DIAGNOSIS BY LOOP-MEDIATED ISOTHERMAL AMPLIFICATION (LAMP) IN THAILAND |
title_full_unstemmed |
MALARIA DIAGNOSIS BY LOOP-MEDIATED ISOTHERMAL AMPLIFICATION (LAMP) IN THAILAND |
title_sort |
malaria diagnosis by loop-mediated isothermal amplification (lamp) in thailand |
publisher |
Universidade de São Paulo |
series |
Revista do Instituto de Medicina Tropical de São Paulo |
issn |
1678-9946 |
publishDate |
2016-01-01 |
description |
The loop-mediated isothermal amplification method (LAMP) is a recently developed molecular technique that amplifies nucleic acid under isothermal conditions. For malaria diagnosis, 150 blood samples from consecutive febrile malaria patients, and healthy subjects were screened in Thailand. Each sample was diagnosed by LAMP, microscopy and nested polymerase chain reaction (nPCR), using nPCR as the gold standard. Malaria LAMP was performed using Plasmodiumgenus and Plasmodium falciparum specific assays in parallel. For the genus Plasmodium, microscopy showed a sensitivity and specificity of 100%, while LAMP presented 99% of sensitivity and 93% of specificity. For P. falciparum, microscopy had a sensitivity of 95%, and LAMP of 90%, regarding the specificity; and microscopy presented 93% and LAMP 97% of specificity. The results of the genus-specific LAMP technique were highly consistent with those of nPCR and the sensitivity of P. falciparum detection was only marginally lower. |
topic |
Malaria Plasmodium Molecular Diagnosis LAMP Thailand |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652016005000220&lng=en&tlng=en |
work_keys_str_mv |
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1724773576674902016 |