Bioinformatics and Phylogenetic Analysis of NADH3 and NADH4L mitochondrial genes in Iranian Camelus bactrianus
Keeping genetic diversity among native Iranian breeds is very important as a national resource. Studying mitochondrial genome between or within breeds can be a useful indicator of genetic diversity of the population to be studied. The aim of current study was bioinformatic and phylogenetic investiga...
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Shahid Bahonar University of Kerman
2015-11-01
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Online Access: | https://jab.uk.ac.ir/article_1139_98267c0346346467982e2f3ae274cffd.pdf |
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doaj-16d665a415b140168f84ef35af36954f2020-11-25T03:29:06ZfasShahid Bahonar University of Kermanمجله بیوتکنولوژی کشاورزی2228-67052228-65002015-11-017316317410.22103/jab.2015.11391139Bioinformatics and Phylogenetic Analysis of NADH3 and NADH4L mitochondrial genes in Iranian Camelus bactrianusAmin ShahabiMojtaba Tahmoures PourKeeping genetic diversity among native Iranian breeds is very important as a national resource. Studying mitochondrial genome between or within breeds can be a useful indicator of genetic diversity of the population to be studied. The aim of current study was bioinformatic and phylogenetic investigation of NADH3 and NADH4L genes of mitochondrial genome in Camelus bactrianus in Iran. For this purpose, blood samples were collected from 10 Camelus bactrianus in Iran. After extracting DNA, fragment 971 bp of genome mitochondrial of Camelus bactrianus amplified by primers. The amplified fragments were sequenced after purification. Results indicated two different haplotypes based on one single nucleotide polymorphism sequence. The final sequences of each haplotype had a length of approximately 715 bp which included 27/70 % adenine, 13/80 % guanine, 25/60 % cytosine and 32/80 % thymine. Comparison of nucleotide and amino acid sequences of NADH3 and NADH4L genes among Iranian Camelus bactrianus demonstrated that this specie had close genetic distance with domestic Camelus bactrianus. Phylogenetic analysis using Neighbor-Joining method showed that this specie has the lowest similarity with Lama among the <em>Camelidae family.</em><em></em>https://jab.uk.ac.ir/article_1139_98267c0346346467982e2f3ae274cffd.pdfnadh3 genenadh4l genecamelus bactrianusgenome mitochondrial |
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language |
fas |
format |
Article |
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DOAJ |
author |
Amin Shahabi Mojtaba Tahmoures Pour |
spellingShingle |
Amin Shahabi Mojtaba Tahmoures Pour Bioinformatics and Phylogenetic Analysis of NADH3 and NADH4L mitochondrial genes in Iranian Camelus bactrianus مجله بیوتکنولوژی کشاورزی nadh3 gene nadh4l gene camelus bactrianus genome mitochondrial |
author_facet |
Amin Shahabi Mojtaba Tahmoures Pour |
author_sort |
Amin Shahabi |
title |
Bioinformatics and Phylogenetic Analysis of NADH3 and NADH4L mitochondrial genes in Iranian Camelus bactrianus |
title_short |
Bioinformatics and Phylogenetic Analysis of NADH3 and NADH4L mitochondrial genes in Iranian Camelus bactrianus |
title_full |
Bioinformatics and Phylogenetic Analysis of NADH3 and NADH4L mitochondrial genes in Iranian Camelus bactrianus |
title_fullStr |
Bioinformatics and Phylogenetic Analysis of NADH3 and NADH4L mitochondrial genes in Iranian Camelus bactrianus |
title_full_unstemmed |
Bioinformatics and Phylogenetic Analysis of NADH3 and NADH4L mitochondrial genes in Iranian Camelus bactrianus |
title_sort |
bioinformatics and phylogenetic analysis of nadh3 and nadh4l mitochondrial genes in iranian camelus bactrianus |
publisher |
Shahid Bahonar University of Kerman |
series |
مجله بیوتکنولوژی کشاورزی |
issn |
2228-6705 2228-6500 |
publishDate |
2015-11-01 |
description |
Keeping genetic diversity among native Iranian breeds is very important as a national resource. Studying mitochondrial genome between or within breeds can be a useful indicator of genetic diversity of the population to be studied. The aim of current study was bioinformatic and phylogenetic investigation of NADH3 and NADH4L genes of mitochondrial genome in Camelus bactrianus in Iran. For this purpose, blood samples were collected from 10 Camelus bactrianus in Iran. After extracting DNA, fragment 971 bp of genome mitochondrial of Camelus bactrianus amplified by primers. The amplified fragments were sequenced after purification. Results indicated two different haplotypes based on one single nucleotide polymorphism sequence. The final sequences of each haplotype had a length of approximately 715 bp which included 27/70 % adenine, 13/80 % guanine, 25/60 % cytosine and 32/80 % thymine. Comparison of nucleotide and amino acid sequences of NADH3 and NADH4L genes among Iranian Camelus bactrianus demonstrated that this specie had close genetic distance with domestic Camelus bactrianus. Phylogenetic analysis using Neighbor-Joining method showed that this specie has the lowest similarity with Lama among the <em>Camelidae family.</em><em></em> |
topic |
nadh3 gene nadh4l gene camelus bactrianus genome mitochondrial |
url |
https://jab.uk.ac.ir/article_1139_98267c0346346467982e2f3ae274cffd.pdf |
work_keys_str_mv |
AT aminshahabi bioinformaticsandphylogeneticanalysisofnadh3andnadh4lmitochondrialgenesiniraniancamelusbactrianus AT mojtabatahmourespour bioinformaticsandphylogeneticanalysisofnadh3andnadh4lmitochondrialgenesiniraniancamelusbactrianus |
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1724580684452855808 |