Bioinformatics and Phylogenetic Analysis of NADH3 and NADH4L mitochondrial genes in Iranian Camelus bactrianus

• Main Authors: Amin Shahabi, Mojtaba Tahmoures Pour
• Format: Article
• Language: fas
• Published: Shahid Bahonar University of Kerman 2015-11-01
• Series: مجله بیوتکنولوژی کشاورزی
• Subjects: nadh3 gene; nadh4l gene; camelus bactrianus; genome mitochondrial
• Online Access: https://jab.uk.ac.ir/article_1139_98267c0346346467982e2f3ae274cffd.pdf

Keeping genetic diversity among native Iranian breeds is very important as a national resource. Studying mitochondrial genome between or within breeds can be a useful indicator of genetic diversity of the population to be studied. The aim of current study was bioinformatic and phylogenetic investigation of NADH3 and NADH4L genes of mitochondrial genome in Camelus bactrianus in Iran. For this purpose, blood samples were collected from 10 Camelus bactrianus in Iran. After extracting DNA, fragment 971 bp of genome mitochondrial of Camelus bactrianus amplified by primers. The amplified fragments were sequenced after purification. Results indicated two different haplotypes based on one single nucleotide polymorphism sequence. The final sequences of each haplotype had a length of approximately 715 bp which included 27/70 % adenine, 13/80 % guanine, 25/60 % cytosine and 32/80 % thymine. Comparison of nucleotide and amino acid sequences of NADH3 and NADH4L genes among Iranian Camelus bactrianus demonstrated that this specie had close genetic distance with domestic Camelus bactrianus. Phylogenetic analysis using Neighbor-Joining method showed that this specie has the lowest similarity with Lama among the <em>Camelidae family.</em><em></em>